Method for extracting and separating fucoxanthin from marine unicellular diatom

A technology for separating fucoxanthin and fucoxanthin is applied in the field of extraction and separation of fucoxanthin, and can solve the problems of low yield of pigment extraction, low fucoxanthin content, affecting pigment extraction and the like , to achieve the effect of short extraction time, short time and fast separation speed

Active Publication Date: 2014-08-06
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) Time-consuming and labor-intensive, the extraction process needs to grind the algal body to improve the extraction efficiency;
[0007] (2) Large seaweeds such as kelp contain a lot of polysaccharides and alginate, which affect the extraction of pigments;
[0008] (3) The content of fucoxanthin in raw materials is not high, and the yield of pigment extraction is not high

Method used

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  • Method for extracting and separating fucoxanthin from marine unicellular diatom
  • Method for extracting and separating fucoxanthin from marine unicellular diatom
  • Method for extracting and separating fucoxanthin from marine unicellular diatom

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Embodiment 1

[0031]The above-mentioned cultured Phaeodactylum tricornutum (Phaeodactylum tricornutum) algae liquid was divided into centrifuge tubes, balanced and put into a centrifuge for centrifugation, the centrifugal force was 3000g, and the centrifugation time was 5 minutes. After the centrifugation was completed, the supernatant was removed, and the algal mud in each tube was combined and weighed. According to the ratio of 5ml / g, add pre-cooled (0-10 degrees) mixture of methanol and acetone as the extraction solution (methanol: acetone = 1:1 (V / V)), shake fully to make the algae cells fully contact with the extraction solution, It was then placed in an ice-water bath for 5 minutes in the dark. During the leaching process, fill nitrogen with a ventilation rate of 3-7L / min to prevent oxidation. The supernatant was collected by centrifugation, and the precipitate was extracted once again with the extraction solution at a ratio of 5 ml / g. Centrifuge and collect the supernatant. The pr...

Embodiment 2

[0037] The cultured Phaeodactylum tricornu tum algae liquid was divided into centrifuge tubes, balanced and loaded into a centrifuge for centrifugation with a centrifugal force of 3000g and a centrifugation time of 5 minutes. After the centrifugation was completed, the supernatant was removed, and the algal mud in each tube was combined and weighed.

[0038] According to the ratio of 5ml / g, add pre-cooled (0-10 degrees) mixture of methanol and acetone as the extraction solution (methanol: acetone = 1:1 (V / V)), fully shake, so that the algae cells can fully contact with the extraction solution , and then placed in an ice-water bath for 5 minutes in the dark. Nitrogen was used to prevent oxidation during the extraction process. The supernatant was collected by centrifugation, and the precipitate was extracted once again with the extraction solution at a ratio of 5 ml / g. Centrifuge and collect the supernatant. The extract was filtered through a filter membrane with a pore size...

Embodiment 3

[0046] The cultured Thalassiosira pseudonana algae liquid was divided into centrifuge tubes, balanced and loaded into a centrifuge for centrifugation with a centrifugal force of 3000g and a centrifugation time of 5 minutes. After the centrifugation was completed, the supernatant was removed, and the algal mud in each tube was combined and weighed.

[0047] According to the ratio of 5ml / g, add pre-cooled (0-10 degrees) mixture of methanol and acetone as the extraction solution (methanol: acetone = 1:1 (V / V)), fully shake, so that the algae cells can fully contact with the extraction solution , and then placed in an ice-water bath for 5 minutes in the dark. Nitrogen was used to prevent oxidation during the extraction process. The supernatant was collected by centrifugation, and the precipitate was extracted once again with the extraction solution at a ratio of 5 ml / g. Centrifuge and collect the supernatant. The extract was filtered through a filter membrane with a pore size o...

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Abstract

The invention relates to extraction and separation of fucoxanthin, and in particular to a method for extracting and separating fucoxanthin from marine unicellular diatom. A fat-soluble extract is added into and mixed with the marine unicellular diatom mud, the mixture is extracted under the conditions of low temperature, darkness and no oxidation for 2-10 min, supernatant extract is collected by centrifugation, the precipitate is repeatedly extracted again by an extract, and the supernatants are merged and separated by liquid chromatography, so as to obtain high-purity fucoxanthin, wherein the usage amount of the extract is 4-6ml for per gram of algae mud. The invention has the advantages of simple operation, fast separation and high yield of fucoxanthin, lays the foundation for the factory production, and ultimately can be used for large-scale industrialized production.

Description

technical field [0001] The invention relates to the extraction and separation of fucoxanthin, in particular to a method for extracting and separating fucoxanthin from marine unicellular diatoms. Background technique [0002] Fucoxanthin, also known as fucoxanthin or fucoxanthin, mainly exists in brown algae and some heteroflagellates (heterokonts), and is an important auxiliary pigment that composes their light-harvesting complexes. Its molecular formula is C 42 h 58 o 6 , CAS registration number: 3351-86-8 [CA INDEXNAME: b,b-Carotene,3-(acetyloxy)-6',7'-didehydro-5,6-epoxy-5,5',6,6' ,7,8-hexahydro-3,5'-dihydroxy-8-oxo-,(3S,3'S,5R,'R,6S,6'R)]; the structural formula is: [0003] [0004] Studies in recent years have shown that fucoxanthin has important medicinal and health value. Japanese scholar Murakami's research in 2002 showed that fucoxanthin is an effective inhibitor of mammalian replicative DNA polymerase (Pola). The research team led by Masashi Hosokawa of H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32
CPCC07D303/32
Inventor 王广策解修俊
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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