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Separating preparative chromatography apparatus comprising serial preparative chromatography column

A technology for chromatographic separation and chromatographic instruments, which is used in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc.

Inactive Publication Date: 2011-04-13
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To solve the technical bottleneck of separation and purification of natural products or chemical components of traditional Chinese medicines to prepare pure compounds

Method used

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  • Separating preparative chromatography apparatus comprising serial preparative chromatography column
  • Separating preparative chromatography apparatus comprising serial preparative chromatography column

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The content and technical method of the present invention can be realized through figure 1 , figure 2 Said, and detailed as follows:

[0026] 1. Sample loading: After the test sample is dissolved in solvent, inject the sample through the manual sampler MI, and wait for separation. If the sample volume is large and it is inconvenient to use the manual sampler for 1-2 injections, you can also load the sample through the pre-column, that is, after the sample is adsorbed by the pre-column packing, the sample is loaded on the column.

[0027] 2. Separation:

[0028] 1) Primary separation column C1 chromatographic column separation: A certain proportion of the eluent will elute the test sample, at this time the two-position three-way solenoid valve BV-3 controls the eluent to the primary separation column C1 chromatographic column for separation. At the same time, the two-position six-way solenoid valve BV-6 controls a fraction F1 of the eluent. The fraction F1 is controlled by t...

Embodiment 2

[0054] In this example, the primary chromatographic separation column C1, the secondary chromatographic separation column C21, the secondary chromatographic separation column C22, and the secondary chromatographic separation column C23 use stainless steel pre-packed column separation preparation columns; separation packing adopts: 1) Four The packing separation preparation column uses one material; 2) According to the separation compound object, the four packing separation preparation columns use different materials; a detailed study of the preliminary separation conditions is required. And use the two-position three-way solenoid valve that matches the first and second-stage separation chromatographic column: BV-3 two-position six-way solenoid valve; BV-4 two-position four-way solenoid valve; MI manual sampler; PC Pre-column; PA, PB infusion pump; detector, fraction collector and other instrument components, according to the attached instructions figure 1 It is combined to form ...

Embodiment 3

[0056] In this example, the primary chromatographic separation column C1, the secondary chromatographic separation column C21, the secondary chromatographic separation column C22, and the secondary chromatographic separation column C23 use a glass column packed separation preparation column; the separation packing adopts: 1) four packs The column separation preparation column uses one material; 2) According to the separation compound object, the four-packed column separation preparation column uses different materials; a detailed study of the preliminary separation conditions is required. And use the two-position three-way solenoid valve that matches the first and second-stage separation chromatographic column: BV-3 two-position six-way solenoid valve; BV-4 two-position four-way solenoid valve; MI manual sampler; PC Pre-column; PA, PB infusion pump; detector, fraction collector and other instrument components, according to the attached instructions figure 1 It is combined to for...

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Abstract

The invention relates to a separating preparative chromatography apparatus comprising a serial preparative chromatography column, which mainly comprises a BV-3 two-position three-way electromagnetic valve, a BV-6 two-position six-way electromagnetic valve, a BV-4 two-position four-way electromagnetic valve, a C1 first-stage chromatographic separation column, a C21 second-stage chromatographic separation column 1, a C22 second-stage chromatographic separation column 2, a C23 second-stage chromatographic separation column 3, a C2n second-stage chromatographic separation column n, an MI manual injector, a PC pre-column, a PA infusion pump and a PB infusion pump, a detector and a fraction collector. The C1 first-stage chromatographic separation column is continuously utilized for separating fractions F1-Fn, a group of C21, C22, C23, ...... C2n are respectively injected into the second-stage chromatographic separation columns for separating and purifying compound monomers; the first-stage chromatographic separation column is serially connected with the second-stage chromatographic separation columns, and the second-stage chromatographic separation columns are connected in parallel for independently separating and purifying the compound monomers; and programs of the BV-3 two-position three-way electromagnetic valve, the BV-6 two-position six-way electromagnetic valve and the BV-4 two-position four-way electromagnetic valve are controlled by a chromatographic working station.

Description

Technical field: [0001] The invention relates to a separation and preparation chromatographic instrument, in particular to a separation and preparation chromatographic instrument formed by continuously using two or more pre-installed separation and preparation columns. Background technique: [0002] The research of natural active compounds or lead compounds is inseparable from extraction and separation. With the development of high-throughput biological activity screening technology (HTS), the separation of natural compounds has become a research bottleneck. As a high-efficiency separation technology, preparative chromatography includes three modes of separation, flushing, replacement and frontier; the chromatographic system includes all liquid-liquid chromatography, liquid-solid chromatography, reversed-phase chromatography and supercritical fluid chromatography; the separation mechanism involves adsorption, distribution, and ion exchange , Molecular exclusion, hydrophobic inter...

Claims

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Application Information

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IPC IPC(8): B01D15/22
Inventor 邱明华李忠荣周琳吴海燕
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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