Full online detection multi-dimensional liquid chromatogram separation system based on same detector

A multi-dimensional liquid chromatography and separation system technology, applied in the direction of material separation, instruments, measuring devices, etc., can solve the problem of not realizing the separation monitoring and automatic transparent control of each dimension, difficult to quickly repeat and meet the needs of sample preparation, and difficult to enrich the column or the cleanliness of the separation column, etc., to achieve the effects of flexible use control, improved resolution, and low system cost

Pending Publication Date: 2018-05-15
DALIAN BOMAI TECH DEV
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AI Technical Summary

Problems solved by technology

[0003] Due to technical limitations, most of the current two-dimensional or multi-dimensional liquid chromatography systems use multiple gradient elution systems, adopt dwell mode or continuous mode, and can only detect and control the separation process of the last one dimension, without realizing the full separation of each dimension. Process monitoring and automatic transparent control, it is also difficult to monitor the cleanliness of all enrichment columns or separation columns, it is difficult to quickly repeat and meet the needs of sample preparation; in some multi-dimensional chromatographic separation systems, each dimensional separation system uses an independent detection device and an independent gradient elution system, the cost of the entire chromatographic separation system is relatively high

Method used

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  • Full online detection multi-dimensional liquid chromatogram separation system based on same detector
  • Full online detection multi-dimensional liquid chromatogram separation system based on same detector
  • Full online detection multi-dimensional liquid chromatogram separation system based on same detector

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Embodiment 1

[0032] Embodiment 1: A kind of structure of multi-dimensional high performance liquid chromatography separation system

[0033] In this embodiment, the enrichment column array A7 has 9 enrichment columns, which are sequentially numbered as the first enrichment column, the second enrichment column, etc. of the enrichment column array A7, and the last one is numbered as the enrichment column array A7 The 9th enrichment column; the enrichment column array B8 is a two-stage enrichment column array, and each enrichment column array has 9 enrichment columns, that is, the enrichment column array B8 has 18 enrichment columns, which are sequentially numbered as enrichment columns The first enrichment column of column array B8, the second enrichment column, etc., the last number is the 18th enrichment column of enrichment column array B8; the liquid chromatography separation column array has 5 separation columns, which are numbered sequentially The first separation column, the second se...

Embodiment 2

[0040] Embodiment 2: The multidimensional liquid chromatography separation system is used for the separation of two types of compounds

[0041] The multi-dimensional liquid chromatography separation system has multiple application modes. In addition to the above-mentioned separation plus enrichment mode, there are also enrichment modes, enrichment plus enrichment modes, enrichment plus separation modes, enrichment plus separation plus enrichment modes, And the joint application of multiple modes.

[0042] The application of separation and enrichment of two types of compounds is taken as an example to illustrate. When the two-position ten-way valve is in state A, there are the following separation and enrichment application modes:

[0043] Enrichment mode: When repeated separation of a certain sample is required, multiple samples are injected through the injection valve, and the samples are selectively enriched on the chromatographic column of the enrichment column array A7. A...

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Abstract

The invention provides a full online detection multi-dimensional liquid chromatogram separation system based on a same detector. The full online detection multi-dimensional liquid chromatogram separation system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid diluent pump, a gradient mixer A, a gradient mixer B, an injection valve, an enriching column array A, an enriching column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position ten-wayvalve and a connecting pipeline; the conversion of the previous-dimension separation state and the current-dimension separation state is realized through the switching of the two-position ten-way valve, so that three dimensions or more than three dimensions of chromatographic separation is realized. Each dimension of chromatographic separation column is selected through the liquid chromatographyseparation column array, full online monitoring and control for multi-dimensional chromatographic separation are realized based on the same gradient elute system and the same detector, and cleanlinessdegree controllability for enriching columns and separation columns is realized. Each dimension of separation is connected through the enriching columns, and the enrichment or gathering of compoundsis assisted by using the diluent pump. According to the system, efficient separation for complex system samples with high separation difficulty is realized by selecting the combinations of different chromatographic stationary phases and moving phases.

Description

technical field [0001] The invention belongs to the technical field of high performance liquid chromatography separation, and relates to a multi-dimensional high performance liquid chromatography separation system based on full online detection of the same detector. Background technique [0002] With the development of separation technology, exploring and separating components in complex sample systems has become a hot research field. Multidimensional liquid chromatography technology can effectively improve the resolution of complex sample component separation by increasing peak capacity, and has become the development direction of fast chromatography separation technology. Multidimensional liquid chromatography technology is a liquid chromatography technology that sequentially injects the eluent of the first-dimensional chromatographic column of the sample into the subsequent dimensional chromatographic column for further separation. This separation technology can utilize ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/08G01N30/20
CPCG01N30/88G01N30/08G01N30/20G01N2030/201
Inventor 李宜珊李涛朱靖博贾磊白朝阳钱红飙李豪徐金帅孙绍琳
Owner DALIAN BOMAI TECH DEV
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