Full online detection multi-dimensional liquid chromatogram separation system based on same detector

Abstract

The invention provides a full online detection multi-dimensional liquid chromatogram separation system based on a same detector. The full online detection multi-dimensional liquid chromatogram separation system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid diluent pump, a gradient mixer A, a gradient mixer B, an injection valve, an enriching column array A, an enriching column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position ten-wayvalve and a connecting pipeline; the conversion of the previous-dimension separation state and the current-dimension separation state is realized through the switching of the two-position ten-way valve, so that three dimensions or more than three dimensions of chromatographic separation is realized. Each dimension of chromatographic separation column is selected through the liquid chromatographyseparation column array, full online monitoring and control for multi-dimensional chromatographic separation are realized based on the same gradient elute system and the same detector, and cleanlinessdegree controllability for enriching columns and separation columns is realized. Each dimension of separation is connected through the enriching columns, and the enrichment or gathering of compoundsis assisted by using the diluent pump. According to the system, efficient separation for complex system samples with high separation difficulty is realized by selecting the combinations of different chromatographic stationary phases and moving phases.

Description

technical field [0001] The invention belongs to the technical field of high performance liquid chromatography separation, and relates to a multi-dimensional high performance liquid chromatography separation system based on full online detection of the same detector. Background technique [0002] With the development of separation technology, exploring and separating components in complex sample systems has become a hot research field. Multidimensional liquid chromatography technology can effectively improve the resolution of complex sample component separation by increasing peak capacity, and has become the development direction of fast chromatography separation technology. Multidimensional liquid chromatography technology is a liquid chromatography technology that sequentially injects the eluent of the first-dimensional chromatographic column of the sample into the subsequent dimensional chromatographic column for further separation. This separation technology can utilize ...

AI Technical Summary

Problems solved by technology

[0003] Due to technical limitations, most of the current two-dimensional or multi-dimensional liquid chromatography systems use multiple gradient elution systems, adopt dwell mode or continuous mode, and can only detect and control the separation process of the last one dimension, without realizing the full separation of each dimension. Process monitoring and automatic transparent control, it is also difficult to monitor the cleanliness of all enrichment columns or separation columns, it is difficult to quickly repeat and meet the needs of sample preparation; in some multi-dimensional chromatographic separation systems, each dimensional separation system uses an independent detection device and an independent gradient elution system, the cost of the entire chromatographic separation system is relatively high

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