Multi-dimensional liquid chromatography separation system

Abstract

The invention provides a multi-dimensional liquid chromatography separation system, comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid dilution pump, a gradient mixer A, a gradient mixer B, a sampling valve, an enriching column array A, an enriching column array B, a fraction collector, a liquid chromatographic separation column array, a detector, a two-position ten-way valve and a connecting pipeline, wherein the two-position ten-way valve switches to realize the transformation between the last-dimensional separation state and the next-dimensional separation state, so as to realize three or more-dimensional chromatographic separation. Respective-dimensional chromatographic separation columns are selected through the liquid chromatography separation column array, thereby, based on the same gradient elution system and the same detector, realizing the full-line monitoring and control ofthe multi-dimensional chromatographic separation, and realizing the controllability of cleanliness degree of the enrichment column and the separation column. Respective-dimensional separations are connected by the enrichment column, and the liquid dilution pump is used to assist in compound enrichment or capture. The system achieves efficient separation of monomer compounds in complex system samples with high separation difficulty by selecting different combinations of chromatographic stationary phases and mobile phases.

Description

technical field [0001] The invention belongs to the technical field of high performance liquid chromatography separation, and relates to a multidimensional liquid chromatography separation system. Background technique [0002] With the development of separation technology, exploring and separating components in complex sample systems has become a hot research field. Multidimensional liquid chromatography technology can effectively improve the resolution of complex sample component separation by increasing peak capacity, and has become the development direction of fast chromatography separation technology. Multidimensional liquid chromatography technology is a liquid chromatography technology that sequentially injects the eluent of the first-dimensional chromatographic column of the sample into the subsequent dimensional chromatographic column for further separation. This separation technology can utilize two or more chromatographic columns with different separation mechanis...

AI Technical Summary

Problems solved by technology

However, the inlet and outlet of the enrichment column array in this system are fixed. When the enrichment column is used as a sample loading column, the flow direction of the eluent is consistent with the flow direction of the mobile phase when the sample was originally enriched, and the sample is generally enriched. Collects near the inlet of the enrichment column, so more mobile phase and more time are required to load the sample in the enrichment column

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