Screening methods

a screening method and screening method technology, applied in combinational chemistry, biochemistry apparatus and processes, chemical libraries, etc., can solve the problems of limited scope, limited commercial success, and relatively little research effort directed at the development of plant traits via other means

Active Publication Date: 2014-04-24
BIOCONSORTIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0085]Furthermore, the evaluation may be performed by the requesting party and / or in the first region. Performing the evaluation in the first region better ensures that the evaluation is accurate and that no unforseen environmental factors that may impact on the plant(s) or the microorganism(s) are not considered.

Problems solved by technology

However, this process is extremely costly, slow, limited in its scope and fraught with regulatory difficulties.
Few commercial successes have eventuated from over two decades of large-scale investment into this technology.
Despite many decades of successful scientific research into the conventional breeding of highly-productive crops and into development of transgenic crops, relatively little research effort has been directed at development of plant traits via other means.
However, the inventors have recognised that the complexity of the plant-microorganism associations that underpin the observable benefits is poorly understood.
However, despite the potential of microorganisms to improve plant growth, commercial success is limited to a relatively small range of specific microbial applications e.g. Rhizobium spp. to legume seeds, or the use of products resulting from “uncontrolled” microbial fermentations e.g. compost teas, seaweed fermentations, fish waste fermentations etc.

Method used

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  • Screening methods

Examples

Experimental program
Comparison scheme
Effect test

example 1

Use of a Method of the Invention Combined with Whole Genome Shuffling to Select Corn-Adapted Bacteria Conferring Resistance to the Corn Root Worm

Step 1: Library Acquisition

[0358]Whole genome shuffling can accelerate directed evolution by facilitating recombination between the members of a selected population of microbes. Diverse strains of Bacillus thuringiensis containing Cry3 genes and other cry genes with activity against the corn root worm (CRW) have been found in numerous habitats. Similarly, multiple species and strains of Bacillus, including B. thuringiensis, have been found in endo-, epi- or rhizospheric association with many crop species, including corn. Multiple strains from both of these sources, i.e. both CRW active and corn-root associative are combined to form a single parental library.

Step 2: Recombination of Bacterial Genomes

[0359]Combinatorial libraries of new strains are generated from the parental library by intraspecific fusion and interspecific hybridization by ...

example 2

Use of a Method of the Invention with Directed Molecular Evolution to Select Corn-Adapted Bacteria Conferring Desirable Crop Output Traits

[0368]Directed evolution of single genes or loci through random or directed mutagenesis techniques and other recombinatorial procedures known to those skilled in the art, can be used to create libraries of microbes exhibiting targeted genetic variation. In this example the process outlined in the invention is applied as a series of iterative steps following creation of the library, to generate crop-adapted strains of microbes conferring the desired phenotype. The methodology is as generally described above with the difference being that specific genes or loci associated with a desired phenotype are targeted.

[0369]Recombinant libraries may be used separately or combined with naturally-occurring microbes derived from any material or step in a process of the invention.

example 3

Use of Process to Select Seed-Borne Endophytes Conveying a Beneficial Crop Trait

[0370]Forage grasses expressing beneficial traits such as insect-resistance and improved tolerance to both biotic and abiotic stressors via strains of the seed-borne fungus Neotypodium sp. have been widely adopted by farmers in Zealand and elsewhere. It would be desirable to extend the benefits of traits similar to those expressed by this seed-borne fungus and other similar species in the fungal family, to a broader range of seed-borne endophytic microbes thereby providing access to a much wider range of beneficial crop traits.

[0371]Step 1. Untreated ryegrass seeds are planted in a wide variety of soils in small pots. Soils may include additional amendments comprising pure cultures of microorganisms, mixtures of microorganisms or materials containing microorganisms that derived from other sources, including those outlined in examples 1 and 2 above.

[0372]Step 2. After a suitable period of growth, the plan...

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Abstract

The invention relates to methods for the screening, identification and / or application of microorganisms of use in imparting beneficial properties to plants. In one embodiment, the method involves subjecting one or more plants to a growth medium in the presence of a set of microorganisms, selecting one or more plant, acquiring one or more microorganisms associated with the selected plant(s) and repeating the process one or more times. The method further involves the step of subjecting the one or more microorganisms to a conditioning and / or directed evolution process.

Description

RELATED APPLICATION[0001]This application is a continuation of U.S. application Ser. No. 14 / 031,461, filed on Sep. 19, 2013, which claims priority from New Zealand Provisional Application No. 602533, filed Sep. 19, 2012, the content of which is hereby incorporated by reference herein.FIELD[0002]The present invention relates to methods for the screening, identification and / or application of microorganisms of use in imparting beneficial properties to plants.BACKGROUND[0003]Geography, environmental conditions, disease and attack by insects are major factors influencing the ability to grow and cultivate different species of plant. Such factors can have a significant downstream economic and social impact on communities around the world. There would be benefit in identifying products and methods which might impart beneficial properties to a plant species to allow it to grow in a variety of geographical locations, in different weather conditions, to survive disease and to be resistant to a...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/10
CPCC12N15/1058
Inventor TURNER, SUSAN JANEWIGLEY, PETER JOHN
Owner BIOCONSORTIA
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