The invention discloses a stem cell collecting device in the field of stem cell collection. The device comprises a box body, wherein the inner wall of the box body is divided into a blood storage chamber and a separation chamber through a partition plate, a rotating shaft is rotationally connected to the center of the top wall of a cover body, a cleaning assembly is arranged above the rotating shaft, the bottom end of the partition plate communicates with a liquid discharging pipe with an electromagnetic valve, and the bottom end of the liquid discharging pipe is rotationally connected and communicates with a stem cell separator. By additionally arranging the cleaning assembly, when the blood storage chamber needs to be cleaned, a rotating ring is rotated to move towards the inner side, avertical rod and an inclined rod are separated from the first cavity and the second cavity correspondingly, a limiting assembly is rotated, and then a sleeve is rotated, so that a first screw penetrates through a through hole and is locked through a nut, clear water is sprayed out of an annular spraying pipe; a first motor drives the cleaning assembly to rotate to clean the inner wall of the bloodstorage chamber, residual blood stains can be fully cleaned under the combined action of the clear water and a cleaning brush, and the cleaning effect is good; the cleaning assembly is not in contactwith blood, and the cleaning brush is prevented from polluting blood.