Wheat fusarium stalk rot seedlings inoculation identification method

A technology of Fusarium graminearum and its identification method, which is applied in the field of plant protection, can solve the problems of no report on the identification method of Fusarium graminearum stem rot, and achieve the effect of broadening the utilization of wheat resources

Inactive Publication Date: 2008-12-17
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

But so far, my country has not reported in detail the wheat stem rot caused by Fusarium g...

Method used

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  • Wheat fusarium stalk rot seedlings inoculation identification method

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Embodiment Construction

[0007] Get the preserved Fusarium graminearum strains and pick a small piece of mycelium on the ultra-clean workbench to inoculate on the prepared PDA medium (potato dextrose agar) for activation and culture for 3 days. Then a small piece of mycelium was picked and inoculated in 0.6% mung bean soup liquid medium (mung bean agar, MBA) after sterilization. Shake culture at 180r / min on a temperature-controlled shaker at 25°C for 72 hours, filter with multi-layer gauze, count with a hemocytometer under a microscope, and adjust the concentration of the bacterial solution to 10 5 spores / mL suspension and stored at 4°C for later use.

[0008] Wheat seeds were sterilized with 0.1% mercuric chloride for 10 minutes, then sterilized with 75% alcohol for 5 minutes, and rinsed with sterile water for 3-4 times. The seeds were germinated in a petri dish under aseptic operation, and a layer of filter paper was placed in the petri dish.

[0009] After the seeds germinated, they were sown in ...

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Abstract

The invention relates to an inoculation and identification method for crown rot caused by fusarium graminearum of wheat at seedling stage, which pertains to the technical field of plant protection and is used for fast identifying and screening wheat germplasm resources with resistance to the crown rot caused by fusarium graminearum from wheat resources in China. After activation, fusarium graminearum is inoculated in a liquid nutrient medium of 0.6 percent of green bean soup which is sterilized with high pressure at a temperature of 121 DEG C, and cultured in a shaker for 72h under a temperature of 25 DEG C, and then fusarium graminearum suspension of 10 <5> spores/ml is obtained; wheat seeds are sprouted after disinfection and are sowed in sterilized earth after germination; inoculation is started 10 days after seedling emergence; the crown rot inspection of wheat stem base is carried out 35 days after inoculation. The inoculation and identification method has the advantages of setting up the inoculation and identification method for crown rot caused by fusarium graminearum of wheat at seedling stage, providing a basis for the research of the crown rot caused by fusarium graminearum, being capable of fast screening wheat resistance resources and improving the utilization of the wheat resources.

Description

technical field [0001] The invention relates to a method for quickly inoculating and identifying wheat Fusarium graminearum stem rot (Crown Rot) seedling stage, in particular to a screening method for wheat stem rot-resistant resource materials, and belongs to the technical field of plant protection. Background technique [0002] Fusarium wheat (Fusarium) is one of the main pathogenic bacteria that endanger the high and stable yield of wheat. Fusarium can cause two major wheat diseases: wheat scab and crown rot. Since the late 1980s, due to the impact of global climate change and farming systems, wheat scab has spread in Europe, Frequent occurrences in the United States, Canada, China, South America and other places have caused serious losses to wheat yield and quality. For this reason, extensive and in-depth research on wheat scab has been carried out, and remarkable achievements have been made. my country is the country with the largest area of ​​wheat scab damage in the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
Inventor 张鹏周淼平马鸿翔余桂红任丽娟张旭霍燕
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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