Application of trichoderma atroviride HB20111 to prevention and treatment of wheat stem rot and banded sclerotial blight

A technology of HB20111, Trichoderma dark green, applied in the application, method of using spores, botanical equipment and methods, etc., can solve the problems that cannot fundamentally improve the aggravation and spread of soil-borne diseases, hidden dangers of environment and food safety, etc. problems, to achieve the effect of improving the quality of agricultural products, reducing the use, and promoting plant growth

Active Publication Date: 2020-03-27
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the current situation that wheat diseases are mainly prevented and controlled by chemical fungicides, which cannot fundamentally improve the aggravation and spread of soil-borne diseases, and have potential safety hazards to the environment and food, the present invention provides a kind of Trichoderma dark green HB20111 in the prevention and treatment of wheat Application on stem rot and sheath blight

Method used

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  • Application of trichoderma atroviride HB20111 to prevention and treatment of wheat stem rot and banded sclerotial blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of high-concentration spore suspension of Trichoderma aurantia HB20111

[0025] Trichoderma dark green HB20111 is obtained in large quantities by solid fermentation method. The specific preparation method includes the following steps:

[0026] ① Preparation of solid primary seeds: Use PDA medium to inoculate the test tube strain of Trichoderma HB20111 in test tube medium, cultivate at 28°C, and store in refrigerator at 4°C. The hyphae and conidia are required to be plump and free of bacteria; Then inoculate the eggplant-shaped flask, use PDA culture medium, culture at 28°C, the bacteria age is 8 days, the surface of the colony is required to be free of liquid water, the hyphae and conidia are plump, and there is no contamination by bacteria;

[0027] ②Liquid seed preparation: Use 1% glucose water sterilized at 115°C for 30 minutes to rinse the surface of the eggplant bottle, wash the fresh spores and dilute to 5×10 6 Spores / mL as liquid seeds for solid in...

Embodiment 2

[0033] Example 2 Preparation of biological seed dressing agent

[0034] Use diatomaceous earth to adsorb the spore suspension of Trichoderma HB20111 prepared in Example 1 to form a solid bacterial agent. Add 3% sodium carboxymethyl cellulose and 3% sodium alginate and mix with a mixer to adjust the bacteria The number is 2.0×10 8 cfu / g, dried in a fluidized bed at 50°C to a moisture content of less than 10%, and then packed into bags.

Embodiment 3

[0070] Use medical stone to adsorb the spore suspension of Trichoderma dark green HB20111 to form a solid bacterial agent. Add 2% sodium carboxymethyl cellulose and 1% sodium alginate and mix with a mixer to adjust the bacterial count to 2.0×10 8 cfu / g, dried in a fluidized bed at 50°C until the moisture content is below 10%, and then packed into bags;

[0071] Among them, the spore suspension of Trichoderma dark green HB20111 is obtained in large quantities by solid fermentation method, and the specific preparation method includes the following steps:

[0072] ① Preparation of solid primary seeds: Use PDA medium to inoculate the test tube strain of Trichoderma HB20111 in test tube medium, cultivate at 28°C, and store in refrigerator at 4°C. The hyphae and conidia are required to be plump and free of bacteria; Then inoculate the eggplant-shaped bottle, use PDA culture medium, culture at 28°C, and the bacteria age is 5 days. It is required that there is no liquid water on the surface...

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Abstract

The invention relates to the technical field of biopesticides, and particularly relates to application of trichoderma atroviride HB20111 to prevention and treatment of wheat stem rot and banded sclerotial blight. The trichoderma atroviride HB20111 is preserved in China General Microbiological Culture Collection Center on December 5, 2018, and the preservation number of the trichoderma atroviride HB20111 is CGMCC No. 16963. The invention belongs to the field of green biopesticides, and the use of the trichoderma atroviride HB20111 can correspondingly reduce the use of chemical pesticides and pesticide residues in agricultural products and has important significance for improving the quality of the agricultural products and is suitable for production and application of pollution-free green agricultural products.

Description

Technical field [0001] The invention relates to the technical field of biological pesticides, and in particular to the application of Trichoderma cyanoderma HB20111 in preventing and treating wheat stem rot and sheath blight. Background technique [0002] Wheat is one of the most widely planted grains in the world and one of the important cash crops in my country. According to data from the National Bureau of Statistics, the wheat planting area in my country in 2018 was 24.27 million hectares and the wheat output was 13.143 million tons. However, with the improvement of wheat production level, the application of chemical fertilizers has increased significantly, and the application of organic fertilizers has been greatly reduced. This has caused soil ecology to deteriorate and soil-borne diseases continue to occur. In addition, the area of ​​continuous cropping and straw return has also increased. The accumulation of pathogenic bacteria in the soil year by year, coupled with the la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/38A01P3/00C12N3/00C12N1/14
CPCC12N1/14C12N3/00
Inventor 扈进冬李纪顺魏艳丽陈凯刘宝军赵忠娟杨凯李玲王贻莲吴远征李红梅杨合同
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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