Production process for fermenting trichoderma spp by taking orange peels as raw materials

A production process, the technology of Trichoderma, applied in biocides, microorganisms, fungicides, etc., can solve the problems of environmental pollution, waste of resources, etc., and achieve the effects of simple preparation method, improved utilization rate, and large spore production

Active Publication Date: 2013-12-25
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, this nutrient-rich substance was discarded, which not only wasted a lot of resources, but also polluted the environment.
At present, the research on citrus peels is mainly concentrated in two aspects: one is the extraction of internal components, including the extraction and utilization of pectin, essential oils, hesperidin, flavonoids, pigments and other substances; the other is microbial fermentation. , mainly fermenting feed and enzyme preparations with citrus peels as nutrients, and the method of producing Trichoderma inoculum with orange peels as a medium has not been reported

Method used

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  • Production process for fermenting trichoderma spp by taking orange peels as raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: orange peel wheat bran combination is to Trichoderma reesei ( Trichoderma ressei ) FS10-C growth-promoting effect

[0021] 1.1 Experimental design

[0022] (1) Preparation of spore suspension: Take the cultured Trichoderma reesei ( Trichoderma ressei ) FS10-C seed slant, add 5mL sterile water, oscillate, elute the spores into a sterile Erlenmeyer flask, dilute to a spore concentration of 10 6 spores / mL for use.

[0023] (2) Solid-state fermentation: Dry the orange peel at 70°C, crush it through a 0.84mm sieve; take 100 parts of orange peel powder, 50 parts of orange peel powder, 50 parts of wheat bran, and 100 parts of wheat bran, according to the weight of feed water Add deionized water at a ratio of 1:1, divide into 15cm petri dishes, about 60g in each petri dish, repeat 3 times, sterilize at 121°C for 30 minutes, cool to room temperature; inoculate Richter scale at a ratio of 5% wt Trichoderma ( Trichoderma ressei ) FS10-C spore suspension, ...

Embodiment 2

[0030] Embodiment 2: the optimization of Trichoderma solid fermentation condition

[0031] 2.1 Experimental design

[0032] (1) Preparation of spore suspension: Take the cultured Trichoderma reesei ( Trichoderma ressei ) FS10-C seed slant, add 5mL sterile water, oscillate, elute the spores into a sterile Erlenmeyer flask, dilute to a spore concentration of 10 6 spores / mL for use.

[0033] (2) Solid-state fermentation: Dry the orange peel at 70°C, crush it through a 0.84mm sieve; select 4 factors including substrate ratio, inoculum size, water content and culture time, take 3 levels respectively, and take L 9 ( 3 4 ) Orthogonal table Carry out orthogonal test, repeat 3 times, inoculate Trichoderma reesei ( Trichoderma ressei ) FS10-C spore suspension, mix well and place in a constant temperature incubator at 28°C for static culture, stirring regularly.

[0034] (3) Determination of sporulation: After the fermentation, accurately weigh 1.0 g of the solid culture, qua...

Embodiment 3

[0040] Embodiment 3: fermentor enlarged culture produces Trichoderma inoculum agent

[0041] Specific steps are as follows:

[0042] (1) Preparation of spore suspension: Take the cultured Trichoderma reesei ( Trichoderma ressei ) FS10-C seed slant, add 5 mL sterile water, oscillate, elute the spores into a sterile Erlenmeyer flask, and dilute to a spore concentration of 10 6 spores / mL for use.

[0043] (2) Preparation of liquid seeds: Weigh 200g of potatoes, cut into pieces, boil and filter to obtain potato juice, add 20g of glucose, add water to make up 1000mL, bottle and sterilize, cool and inoculate the above spore suspension at a ratio of 2%wt, 28°C, 150°C Turn per minute and cultivate for 3 days to obtain liquid seeds.

[0044](3) Solid-state fermentation: Dry the orange peel at 70°C and crush it through a 0.84mm sieve; take 50 parts of orange peel powder and 50 parts of wheat bran, add deionized water to adjust according to the weight ratio of material to water 1:0...

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Abstract

The invention discloses a production process for fermenting trichoderma spp by taking orange peels as raw materials, which belongs to the technical field of microorganisms. The production process comprises the following steps: (1) preprocessing: drying, grinding and sieving the orange peels; (2) mixing preprocessed orange peel powder with wheat bran and de-ionized water according to a certain ratio, and sterilizing by high-pressure steam; (3) inoculating trichoderma onto a sterilized material for solid-state fermentation; (4) drying a solid fermentation product to obtain a trichoderma preparation product. The trichoderma spp preparation produced by the process has capabilities of promoting plant growth and inducing plants to resist adversity and diseases, can be used for preventing and treating soil-borne diseases and promoting repair of plants in polluted soil, and provides a new way for resource utilization of the orange peels.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for producing trichoderma fungus by solid-state fermentation of orange peel. [0002] Background technique [0003] Trichoderma ( Trichoderma spp ) is widely distributed in nature, with large biomass and strong environmental adaptability. It is a biocontrol fungus with a long history. At present, there are more than 50 kinds of Trichoderma fungus products that have been developed and registered internationally, most of which can be used to control crops Soilborne diseases and promotion of plant growth. In recent years, studies have also shown that Trichoderma has the function of adsorbing heavy metals, and it has gradually been applied to the combined plant-microbe remediation technology of polluted soil. Since Trichoderma is a live fungus preparation, its biological activity is mainly achieved through spores. Therefore, choosing a cheap fer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00A01N63/04A01P1/00A01P3/00B09C1/10
Inventor 滕应罗洋骆永明马文亭李振高
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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