Chlamydospore obtained through liquid fermentation of trichoderma spp and preparation method of chlamydospore

A technique for chlamydospore and liquid fermentation, which is applied in the field of chlamydospore and its preparation, can solve the problems of high production cost, limited application, and low spore production, and achieves improvement in popularization and application, increased spore production, and reduced production cost. Effect

Pending Publication Date: 2019-12-20
YANGZHOU POLYTECHNIC INST
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Problems solved by technology

The development of Trichoderma chlamydospore preparations is an effective way to improve the storage and biocontrol performance of Trichoderma preparations, but it is difficult to induce Trichoderma to produce a large number of chlamydospores, and the amount of sporulation is small, and the production cost is high, which greatly limits Its application needs to be improved

Method used

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  • Chlamydospore obtained through liquid fermentation of trichoderma spp and preparation method of chlamydospore
  • Chlamydospore obtained through liquid fermentation of trichoderma spp and preparation method of chlamydospore
  • Chlamydospore obtained through liquid fermentation of trichoderma spp and preparation method of chlamydospore

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Embodiment Construction

[0019] 1. Production of culture medium

[0020] 1.1 Enzymatic hydrolysis of corn stover

[0021] Weigh 28.0-30.0g of corn stalks that have been hydrothermally pretreated (see CN201611194437.9 for the method) and crush them to 40 mesh (extremely dry substrate), place them in a 500ml tripod bottle, and add 150ml HAc-NaAc buffer (pH=4.8) , Concentration 0.1mol / L), add 1.2ml of tetracycline hydrochloride solution with a concentration of 10mg / ml, add 15ml of NNO (sodium methylene dinaphthalene sulfonate) solution with a concentration of 0.5% and 15ml of JFC- with a concentration of 0.2% S solution, add 15FPU / g substrate cellulase and 1.5CBU / g substrate cellobiase at the same time, add distilled water to a total liquid volume of 300ml, add 20 glass balls, and cover the bottle stopper. After mixing uniformly, put the Erlenmeyer flask into the shaker for enzymatic hydrolysis, the temperature is set to 49-50℃, the rotating speed is 180r / min, the enzymatic hydrolysis time is 80-85h, the enz...

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Abstract

The invention relates to chlamydospore obtained through liquid fermentation of trichoderma spp and a preparation method of the chlamydospore. The chlamydospore is obtained through the steps of using corn straw enzymatic hydrolysate as a culture medium raw material, wherein the straw enzymatic hydrolysate contains sugar components, dispersing agent components and the like, inoculating straw enzymatic hydrolysate culture mediums with trichoderma spp cakes, and performing liquid fermentation so as to obtain the chlamydospore. According to the chlamydospore and the preparation method thereof disclosed by the invention, the corn straw enzymatic hydrolysate is used as a main raw material, by methods of adding vitamins, trace elements, dispersing agents and the like, the spore production quantityof the chlamydospore is substantially increased, the production cost is reduced, the fermentation period is also shortened by 2 days, the working and production efficiency is improved, besides, the shelf life can reach 1 year, and in addition, promotion and application of a trichoderma spp agent are guaranteed.

Description

Technical field [0001] The invention relates to a chlamydospore produced by liquid fermentation of Trichoderma and a preparation method thereof, and belongs to the technical field of biological pesticides and preparation thereof. Background technique [0002] Because Trichoderma has significant biocontrol effects on plant diseases, has good environmental compatibility, and is easy to produce in large quantities, countries all over the world try to develop it as a commercial biological pesticide formulation. According to statistics, about 60% of the active ingredients of all registered biological pesticides in the world are Trichoderma conidia. Such as Topshield (Trichoderma harzianum T22) in the United States, Trichodex (Trichoderma harzianum T39) in Israel, and Trichoderma WP (T.Viride) in China. As mentioned above, most of the commercially available Trichoderma preparations are meristem preparations. However, due to the weak stress resistance and poor storage resistance of co...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/885
CPCC12N1/14C12N3/00
Inventor 田连生陈菲
Owner YANGZHOU POLYTECHNIC INST
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