13 results about "Halobacterium salinarium" patented technology

The invention relates to the field of microbial fermentation engineering, and particularly discloses a stirring paddle combination method and system for optimizing fermentation of halophilic bacteria to produce Ectoine. Through CFD simulation and comparison of eight three-layer paddle combinations, it is found that a CD6-RT-RT combination with a semicircular tube paddle (CD6) arranged on the bottom layer and six flat blade paddles (RTs) arranged on the middle layer and the top layer can synergistically improve the gas-liquid mass transfer efficiency of a high-salt system, bubbles are broken through strong radial flow of the CD6 paddle on the top layer, and a gas-liquid interface is expanded in combination with high-shear axial flow of the RT paddles on the middle layer and the bottom layer; uniform distribution of the gas content (gradient is less than or equal to 5%) and kLegt are realized; compared with a traditional combination, the efficiency is improved by 25%-72%; in a 50L tank, the yield of Ectocaine reaches 32.2 g / L, which is obviously improved by 15.0% compared with that of a conventional RT-RT-RT combination. According to the method, the oxygen transfer bottleneck of high-salt fermentation is improved, and an efficient and low-consumption scheme is provided for industrial production of Ectoin.

The invention belongs to the technical field of microorganisms, and particularly relates to halophilic bacteria for high-yield biomacromolecular materials and high-efficiency expression application of the halophilic bacteria. The invention provides a halophilic bacterium PYL-X1 with high protein yield, the halophilic bacterium PYL-X1 is classified and named as halophilic bacteria (Halophiles) and is preserved in Guangdong Microbial Culture Collection Center on May 6, 2025, and the preservation number is GDMCC No: 66258; the halophilic bacteria PYL-X1 with high protein yield can efficiently produce various recombinant proteins after being introduced into a recombinant protein expression plasmid system, the shake flask level yield is as high as 210 mg / L-300 mg / L, the yield of recombinant fibronectin in a 5L fermentation tank is as high as 7.3 g / L, a new microbial resource is provided for efficient preparation of recombinant proteins, and the halophilic bacteria PYL-X1 has a better industrial application prospect.

The invention relates to an Ectoine-producing halophilic bacterium as well as a screening method and application thereof, and belongs to the technical field of microorganisms. According to the Ectoin-producing halophilic bacteria disclosed by the invention, the Ectoin-producing halophilic bacteria are Vreelandelassp., and the Ectoin-producing halophilic bacteria are Ectoin-producing halophilic bacteria. The 16S rRNA of the Ectoin-producing halophilic bacteria has at least 90% of nucleotide sequence consistency with that of SEQ ID NO. 1; the Ectoin halophilic bacteria are used for expressing 2-aminobutyric acid acetyltransferase, 2-aminobutyric acid transaminase and Ectoin synthetase. Through an efficient screening method and deep genome analysis, the novel halophilic strain capable of synthesizing the Ectoine is successfully screened out, and the gene cluster for synthesizing the Ectoine is excavated, so that the production efficiency and yield of the Ectoine can be improved, a microbial resource library is enriched, and the genetic diversity is increased.

The invention discloses a recombinant halomonas for producing mevalonic acid. The recombinant halomonas is used for expressing an HMG-CoA synthetase gene, namely an EfmvaS gene, from enterococcus faecalis and an HMG-CoA reductase gene, namely an HMGR gene, from rhodobacter marinus. The invention also discloses a preparation method of the recombinant halomonas for producing the mevalonic acid. The recombinant halomonas is used for producing the mevalonic acid. The recombinant halomonas is used for producing the mevalonic acid, and the recombinant halomonas is used for producing the mevalonic acid, wherein the recombinant halomonas is used for expressing the HMG-CoA synthetase gene, namely the EfmvaS gene, from enterococcus faecalis; the invention also relates to a method for producing mevalonic acid. The method comprises the step of fermenting and culturing the recombinant halomonas disclosed by the invention.

The invention belongs to the field of new daily chemical materials, and discloses a sensitive skin anti-aging composition, application and cosmetics, the composition comprises a halophilic bacteria fermentation product and acetyl hexapeptide-8; the weight ratio of the halophilic bacteria fermentation product to the acetyl hexapeptide-8 is 1: (0.1-10). The composition has no irritation, is suitable for sensitive skin, has good improvement effects on crow's feet, subocular wrinkles and compactness, and is very high in improvement speed.

The present application relates to the technical field of biological fermentation, and particularly relates to a cell wall breaking wastewater treatment method and application thereof in halophilic bacteria fermentation, wherein the cell wall breaking wastewater is obtained by cell wall breaking of halophilic bacteria fermentation liquor, and the treatment method comprises the following steps: performing rough filtration on the cell wall breaking wastewater to remove solid suspensions and insoluble impurities in the cell wall breaking wastewater, and then performing membrane filtration on the cell wall breaking wastewater by using a nanofiltration membrane with a cut-off average molecular weight of 700 Da or less, so as to obtain a salt-containing clear liquid; further, the salt-containing clear liquid can be partially or entirely used as a solvent of a fermentation culture medium for halophilic bacteria fermentation process. Through the specific wastewater treatment method, the cell wall breaking wastewater in the halophilic bacteria fermentation process can be recycled, and the PHA yield can be effectively ensured, and even the growth rate of the production strain can be improved.

The invention discloses a recombinant halomonas for producing D-pantothenic acid. The expression of the recombinant halomonas is derived from a ketone pantoic acid hydroxymethyltransferase gene (panB) and a pantothenic acid synthetase gene (panC) of Acetobacter aceti (Acetobacter aceti). Optionally, an endogenous phosphoenolpyruvate carboxylase gene (ppc) is also knocked out from the recombinant halomonas, so that the yield of D-pantothenic acid is further increased. The invention also relates to a method for producing D-pantothenic acid. The method comprises the step of fermenting and culturing the recombinant halomonas disclosed by the invention.

This invention provides a method for synthesizing ectoine using moderately halophilic bacteria through fermentation. It innovatively introduces a concentration reaction system, integrating the "cultivation + concentration" of halophilic bacteria into one process. This eliminates the traditional steps of batch feeding and concentration, greatly increasing the yield and efficiency of ectoine synthesis. It enables inexpensive wild-type halophilic bacteria to achieve a level comparable to that of expensive genetically engineered halophilic bacteria, thus significantly reducing the cost of ectoine synthesis.

The invention relates to a recombinant halomonas. The recombinant halomonas comprises an alsS (acetolactate synthase) gene, an ilvC (acetohydroxyacid isoreductase) gene, an ilvD (dihydroxy acid dehydratase) gene and an ilvE (transaminase) gene which are externally introduced. According to the recombinant halomonas disclosed by the invention, the L-valine can be efficiently produced without sterilization, in an energy-saving and water-saving manner through open fermentation, and the industrial production of the L-valine is facilitated.

To provide a novel method for producing heme iron-containing cytochrome c peroxidase.SOLUTION: A method for producing cytochrome c peroxidase includes: a PHB accumulation step of aerobically culturing a halophilic bacterium belonging to the genus Halomonas in the medium for 10 hours or more to cause the halophilic bacterium to accumulate poly-3-hydroxybutyrate; a 3HB secretion step of microaerobically culturing the halophilic bacterium in the medium while adjusting the pH to cause the poly-3-hydroxybutyrate accumulated within the halophilic bacterium to be secreted into the medium as 3-hydroxybutyrate; and a recovery step of recovering the cytochrome c peroxidase from the medium. The pH during microaerobic culture is adjusted to 9.0 or more and 12.0 or less.SELECTED DRAWING: None

This invention provides a MOF-based halophilic microbial inoculant, its preparation method, and its application, relating to the field of microbial technology. The preparation method of the MOF-based halophilic microbial inoculant of this invention includes the following steps: mixing a halophilic bacterial suspension with a metal-organic framework (MOF) for reaction; then coating the surface of the microbial cell with the MOF to form a MOF shell with a thickness of 0.01–6.0 μm, thereby obtaining the MOF-based halophilic microbial inoculant. The MOF-based halophilic microbial inoculant of this invention, through the organic combination between the halophilic bacterial strain and the metal-organic framework, can reduce the impact of external stimuli on the activity of the halophilic strain, maintain the activity of the halophilic strain under high salt stress conditions, and thus alleviate the damage caused by salt stress to plants.

The method provided by the invention can be used for effectively synthesizing a high 3-hydroxyvalerate monomer (3-HV) in a proportion. The method comprises the following steps: adding a 3-HV precursor substance into a fermentation system to prepare PHBV; the precursor substance is one or more of short-chain fatty acids (VFAs). By the adoption of the method, it is found that Halomonas venusta has high tolerance to VFAs, so that the yield of PHBV can be increased; and the proportion of the 3-HV monomer in the product PHBV can be adjusted by changing the mixing proportion of the substrate. Finally, the content of the obtained PHBV is as high as 90.81 wt%, the proportion of 3-HV monomers is as high as 49.81%, and the content of the obtained PHBV is far higher than that of other non-engineering pure bacterium fermentation.

The present invention relates to a recombinant microorganism comprising a metabolic pathway for the synthesis of P34HB (poly-3-hydroxybutyric acid (3HB)-4-hydroxybutyric acid (4HB) ester) wherein the proportion of 4HB is increased by attenuating the synthesis of 3HB, thereby reducing the addition of precursor substances, achieving the purpose that the strain can grow normally and produce a high proportion of P34HB.