7 results about "Halobacterium salinarium" patented technology

The invention relates to an Ectoine-producing halophilic bacterium as well as a screening method and application thereof, and belongs to the technical field of microorganisms. According to the Ectoin-producing halophilic bacteria disclosed by the invention, the Ectoin-producing halophilic bacteria are Vreelandelassp., and the Ectoin-producing halophilic bacteria are Ectoin-producing halophilic bacteria. The 16S rRNA of the Ectoin-producing halophilic bacteria has at least 90% of nucleotide sequence consistency with that of SEQ ID NO. 1; the Ectoin halophilic bacteria are used for expressing 2-aminobutyric acid acetyltransferase, 2-aminobutyric acid transaminase and Ectoin synthetase. Through an efficient screening method and deep genome analysis, the novel halophilic strain capable of synthesizing the Ectoine is successfully screened out, and the gene cluster for synthesizing the Ectoine is excavated, so that the production efficiency and yield of the Ectoine can be improved, a microbial resource library is enriched, and the genetic diversity is increased.

The present application relates to the technical field of biological fermentation, and particularly relates to a cell wall breaking wastewater treatment method and application thereof in halophilic bacteria fermentation, wherein the cell wall breaking wastewater is obtained by cell wall breaking of halophilic bacteria fermentation liquor, and the treatment method comprises the following steps: performing rough filtration on the cell wall breaking wastewater to remove solid suspensions and insoluble impurities in the cell wall breaking wastewater, and then performing membrane filtration on the cell wall breaking wastewater by using a nanofiltration membrane with a cut-off average molecular weight of 700 Da or less, so as to obtain a salt-containing clear liquid; further, the salt-containing clear liquid can be partially or entirely used as a solvent of a fermentation culture medium for halophilic bacteria fermentation process. Through the specific wastewater treatment method, the cell wall breaking wastewater in the halophilic bacteria fermentation process can be recycled, and the PHA yield can be effectively ensured, and even the growth rate of the production strain can be improved.

This invention provides a method for synthesizing ectoine using moderately halophilic bacteria through fermentation. It innovatively introduces a concentration reaction system, integrating the "cultivation + concentration" of halophilic bacteria into one process. This eliminates the traditional steps of batch feeding and concentration, greatly increasing the yield and efficiency of ectoine synthesis. It enables inexpensive wild-type halophilic bacteria to achieve a level comparable to that of expensive genetically engineered halophilic bacteria, thus significantly reducing the cost of ectoine synthesis.

The invention relates to a recombinant halomonas. The recombinant halomonas comprises an alsS (acetolactate synthase) gene, an ilvC (acetohydroxyacid isoreductase) gene, an ilvD (dihydroxy acid dehydratase) gene and an ilvE (transaminase) gene which are externally introduced. According to the recombinant halomonas disclosed by the invention, the L-valine can be efficiently produced without sterilization, in an energy-saving and water-saving manner through open fermentation, and the industrial production of the L-valine is facilitated.

This invention provides a MOF-based halophilic microbial inoculant, its preparation method, and its application, relating to the field of microbial technology. The preparation method of the MOF-based halophilic microbial inoculant of this invention includes the following steps: mixing a halophilic bacterial suspension with a metal-organic framework (MOF) for reaction; then coating the surface of the microbial cell with the MOF to form a MOF shell with a thickness of 0.01–6.0 μm, thereby obtaining the MOF-based halophilic microbial inoculant. The MOF-based halophilic microbial inoculant of this invention, through the organic combination between the halophilic bacterial strain and the metal-organic framework, can reduce the impact of external stimuli on the activity of the halophilic strain, maintain the activity of the halophilic strain under high salt stress conditions, and thus alleviate the damage caused by salt stress to plants.

The method provided by the invention can be used for effectively synthesizing a high 3-hydroxyvalerate monomer (3-HV) in a proportion. The method comprises the following steps: adding a 3-HV precursor substance into a fermentation system to prepare PHBV; the precursor substance is one or more of short-chain fatty acids (VFAs). By the adoption of the method, it is found that Halomonas venusta has high tolerance to VFAs, so that the yield of PHBV can be increased; and the proportion of the 3-HV monomer in the product PHBV can be adjusted by changing the mixing proportion of the substrate. Finally, the content of the obtained PHBV is as high as 90.81 wt%, the proportion of 3-HV monomers is as high as 49.81%, and the content of the obtained PHBV is far higher than that of other non-engineering pure bacterium fermentation.

The present invention relates to a recombinant microorganism comprising a metabolic pathway for the synthesis of P34HB (poly-3-hydroxybutyric acid (3HB)-4-hydroxybutyric acid (4HB) ester) wherein the proportion of 4HB is increased by attenuating the synthesis of 3HB, thereby reducing the addition of precursor substances, achieving the purpose that the strain can grow normally and produce a high proportion of P34HB.