50 results about "Halobacterium salinarium" patented technology

The invention discloses a preparation of efficient phenol-degrading halophilic bacteria and an application thereof, belonging to the technical field of treatment of waste water and sewage. In the invention, halophilic bacteria in the sewage or sludge are taken as a bacteria source; a gradient domestication method is adopted to screen efficient phenol-degrading halophilic bacteria which have strong degradation capability, can adapt to higher salt concentration and can grow well by taking phenol as the sole carbon source in the phenol-containing waste water with the salt content of 5-6% and thephenol concentration of 100-500mg / L; under the environment of high salt and high phenol, the phenol-degrading performance of the mycelium has obvious advantages over that of the traditional activatedsludge method. The strain can be applied to phenol-degrading treatment of waste water to determine the optimal water power retention time and the optimal bacteria input amount and batch processing time, and the operational conditions can be optimized through orthogonal experiments. The screening of the efficient phenol-degrading halophilic bacteria has high application value, and can provide a reference for the removal of phenol in the oil extraction waste water and the industrial treatment of the phenol-containing wastewater with high content of salt in petrochemistry industry.

The invention discloses a production method of a fresh-keeping sausage casing. The production method of the fresh-keeping sausage casing is characterized in that in the sausage pickling working procedure, a sausage casing preservative which is 2-10% of the mass of the sausage casing in sausage coating salt is added, and then pickling is carried. The sausage casting preservative provided by the invention can effectively inhibit the growth and breeding of sausage casing halophilic bacteria; moreover, natural extractives are stable in nature, are safe to use, and guarantee the quality of the sausage casing well; three kinds of fresh-keeping reagents with anticorrosion and bacterial inhibition effects are adopted; meanwhile, the breeding of the sausage casing halophilic bacteria is inhibited effectively by utilizing the synergistic effect of the preservatives, thereby preventing the red sausage coating salt phenomenon and protecting the color of the sausage casing; a biological fresh-keeping agent is low in cost, is less in using quantity, and is convenient to prepare; the red stain phenomenon in the sausage storage and transportation process is reduced; the quality of the sausage casing is protected; the economical loss is reduced; the production efficiency is increased; the biological fresh-keeping agent of the sausage casing is added in the stage of charging salt for sausage casing and picking so as to effectively inhibit the breeding of the halophilic bacteria; the process is simple, and the production cost is low.

The invention discloses a halophilic bacteria fungicide as well as preparation thereof, a biological treatment system containing the fungicide and an application of the fungicide in treating triazine wastewater. The halophilic bacteria fungicide is prepared by separating and screening degrading bacteria of four triazine compounds, and preparing according to a certain proportion, wherein the degrading bacteria include (Salinicoccus roseus )BJ-1, (Brevibacterium iodinum)BJ-2, Bacillusnitritophilus BJ-3, (Halomonasalkaliphila)BJ-4. The fungicide disclosed by the invention has stronger ability of degradation on the triazine wastewater and can be adaptive to a high-salt environment, has a very good degradation effect under conditions with salt content of 1%-15% and atrazine concentration less than 500mg / l, has obvious advantages in performances of degrading the triazine compounds compared with the traditional active sludge method, and has a degradation effect of over 98% for triazine compounds in the wastewater.

The invention provides a biofertilizer containing amino-oligosaccharin and fermented animal dung. The biofertilizer comprises the following raw materials: fulvic acid, peat, tartaric acid, fermented cow dung, broad bean shell, chloroprene rubber powder, amino-oligosaccharin, Alternaria tenuissima activated protein, D-limonene, mung bean plant, medical stone, nano anion powder, wheat dietary fiber, Lactobacillus zeae, and Halobacterium salinarium. The invention also provides a preparation method of the fertilizer. After application of the fertilizer to saline-alkali soil, six months later, 0-30cm soil layer salinity is 0.18-0.34%, the soil pH is 7.0-7.5, the total nitrogen content of the soil is 0.091-0.127%, the breathable porosity of the soil is 13-17%, the mechanical stability of 5-7mm water-stable granules in the soil is 507-515g, the water-holding capacity of the soil is 20-28%, and the available potassium content of the soil is increased by 45-60%.

A 3-hydroxypropionic acid(3HP) degradation way of halophilic bacteria is analyzed for the first time by a transcriptomics method, the inventor finds that the 3-hydroxypropionic acid can degrade genesdddA and dddC, and recombination halophilic bacteria TD delta dddA which cannot degrade 3HP is obtained. Based on this, an alcohol dehydrogenase gene AdhP and an aldehyde dehydrogenase gene AldDTD, which are from the halophilic bacteria endogenous and responsible for production and metabolism of the 3HP are further utilized, and a synthetizing biology means is taken for adjusting and controlling the expression level of the metabolic way. Through screening, the recombination halophilic bacteria high in yield of the 3HP is obtained, and the yield and the productivity are respectively 0.93g / g ofthe 1,3-propylene glycol(PDO) and 2.4g / L / h, which are the highest 3HP yield and productivity reported so far.

The invention relates to soil ecological restoration conditioner and a preparation method thereof and a soil ecological restoration method, and belongs to the technical field of soil ecological restoration. The technical scheme includes that the soil ecological restoration conditioner comprises, by weight, 1-5 parts of fly maggot chitin, 1-5 parts of actinomyces, 2-6 parts of bacillus, 1-5 parts of photosynthetic bacteria, 2-6 parts of phosphorous and potassium decomposer, 1-3 parts of halophilic bacteria, 30-50 parts of cow dung, 5-15 parts of zeolite, 5-15 parts of medical stone, and 20-50 parts of slag. The soil ecological restoration conditioner is low in cost in favor of popularization and application, and soil restoration effect is excellent without secondary pollution. After soil organism is degraded and restored, planting can realize again in the same year, yield is higher than that of normal soil, and resultantly, the soil ecological restoration conditioner is worthy of wide application.

The invention provides a halophilic bacterium with the preservation number of CGMCC No.22795 and the preservation date of June 28, 2021. The invention further provides a preparation method of the halophilic bacterium, a method for producing PHA by culturing the halophilic bacterium with a low-salt culture medium and an application of the halophilic bacterium. In the method, the used halophilic bacterium can be obtained by breeding means of mutagenesis screening and molecular modification, and a method for producing PHA containing multiple monomers by adding a non-related carbon source (a substrate, such as glucose, which is not directly related to a microbial PHA structure) is realized in the low-salt culture medium, and the proportion of each monomer is adjusted according to the type of the carbon source. According to the method, the problems that traditional halophilic bacteria culture and separation steps are complicated, downstream high-salt sewage is difficult to treat and the like can be solved, and PHA is produced through open fermentation.

The present invention discloses a halomonas aydingkolgenesis strain and an application thereof. The strain is halomonas aydingkolgenesis M1 and has a deposit number of CGMCC NO.19880 in the China General Microbiological Culture Collection Center. Studies show that the halomonas aydingkolgenesis M1can be cultured without sterilization under fermentation conditions in an opening way and can efficiently accumulate polyhydroxyalkanoate (PHA) and / or a small molecule compound of tetrahydropyrimidine, etc. A method for continuous culture of the halomonas aydingkolgenesis M1 is established and a method for co-producing the PHA and tetrahydropyrimidine is successfully established. The method by using the halomonas aydingkolgenesis M1 to produce the PHA and / or tetrahydropyrimidine can realize high yield, is low in production cost, and has broad application prospects.

The invention discloses saline-alkali soil microorganism bacterial manure. The saline-alkali soil microorganism bacterial manure comprises, by mass, 4-9 parts of corncobs, 5-10 parts of sweet potato residues, 13-16 parts of fulvic acid, 10-14 parts of desulfurated waste, 5-7 parts of charcoal, 12-18 parts of vinyl acetate and dibutyl maleate copolymers, 6-8 parts of polyethylene glycol, 2-6 parts of microalgae powder, 4-9 parts of red prince weigela powder, 2-5 parts of exiguobacterium aurantiacum, 3-5 parts of halobacterium salinarium and 0.6-0.9 part of water-retaining agent. The invention further discloses application of the saline-alkali soil microorganism bacterial manure in red fescue planting. The saline-alkali soil microorganism bacterial manure has the advantages that after 12 months when red fescue is planted, the salt content of a soil layer of 0-30 cm is 0.21-0.29%, the pH of the soil is 6.8-7.2, the total nitrogen content in the soil is 0.125-0.154%, the number of ventilation holes in the soil is increased, and the water content of the oil is increased.

Protein-based photovoltaic cells and the manufacture and use of protein-based photovoltaic cells are described. In one embodiment, bacteriorhodopsin from Halobacterium salinarum, which undergoes structural transitions when irradiated with a given wavelength of light, is used as the protein in the protein-based photovoltaic cells. In another embodiment, mutant bacteriorhodopsin from H. salinarum is used. Exposure of the protein to sunlight causes proton transfer across a membrane resulting in the generation of an electrical charge. The protein can be oriented and / or layered on a substrate and modified by mutation to enhance transmembrane proton transfer, covalent binding to a substrate and layering. The protein-based photovoltaic cells sequentially or simultaneously generate hydrogen gas from water or salt, which also can be harnessed to produce electricity.

An SOS gene has been isolated through PCR cloning from Thellungiella halophila and is designated TSOS1. The polynucleotide encodes a transmembrane protein with similarities to plasma membrane Na+ / H+ antiporters from bacteria and fungi. The invention encompasses the TSOS1 gene, the corresponding protein, closely related polypeptides that confer salt tolerance, and related polynucleotides that encode a polypeptide that has Na+ / H+ antiporter activity.

The invention relates to a high-paraffin high-temperature high-salinity oil well microorganism paraffin removing and inhibiting agent and a preparation method thereof, and belongs to the technical field of tertiary oil recovery of an oil field. The microorganism paraffin removing and inhibiting agent is prepared from the following components in percentage by weight: 5 to 10 percent of halobacterium salinarium, 5 to 10 percent of hyperthermophilic archaeon, 8 to 15 percent of paraffin-thirsty bacteria, 20 to 25 percent of industrial brine, 5 to 10 percent of starch, 0.5 to 3 percent of peptone,0.5 to 3 percent of yeast extract and the balance of water. The preparation method of the microorganism paraffin removing and inhibiting agent comprises the following steps: adding the industrial brine, the starch, the peptone and the water into a fermentation tank, regulating pH (Potential of Hydrogen) value of a solution, rising the temperature to 40 to 55 DEG C, uniformly stirring, then heating to 125 DEG C, and sterilizing for 25 minutes; adding the yeast extract, the halobacterium salinarium, the hyperthermophilic archaeon and the paraffin-thirsty bacteria after cooling to 40 to 60 DEG C, fermenting for 48 hours by keeping the temperature be 55 to 60 DEG C, and cooling to room temperature, thus obtaining the microorganism paraffin removing and inhibiting agent. According to microorganism paraffin removing and inhibiting agent and the preparation method thereof, disclosed by the invention, the problems of low high-temperature high-salinity tolerance and poor paraffin removing andinhibiting effect of microorganism paraffin removing and inhibiting strains at present can be effectively solved.

The invention relates to a method for carrying out raw soil greening on heavy saline and alkaline land by using a brassinolide-containing soil conditioner. The brassinolide-containing soil conditioner comprises, by weight, 25-27 parts of fermented sheep dung, 15-17 parts of fermented pig dung, 17-19 parts of fermented duck dung, 2-4 parts of fermented sawdust, 5-7 parts of ammonium sulfate, 4-6 parts of ammonium superphosphate, 3-5 parts of potassium chloride, 5-7 parts of ammonium bicarbonate, 2-4 parts of ammonium phosphate, 2-4 parts of ammonium chloride, 1-3 parts of gel powder, 1-3 parts of diatomite, 1-3 parts of pentanoic acid, 1-3 parts of amino acid salts, 0.5-0.7 parts of brassinolide, 0.5-0.7 parts of compound sodium nitrophenolate, 0.5-0.7 parts of Radix Scutellariae extract, 2-4 parts of tricalcium phosphate, 0.5-0.7 parts of a cellulose grafted acrylate polymer, 0.1-0.3 parts of Halobacterium salinarium, 0.1-0.3 parts of Bacillus coagulans, 0.1-0.3 parts of Trichoderma Longibrachiatum and 0.1-0.3 parts of fermented tea leaves.

The invention discloses a method for synthesizing a biodegradable mulching film from straws and illegal cooking oil by using halomonas sp.ZY-1 under an open and non-sterile condition. Straws are treated to obtain a straw supernatant, and an MS culture medium with the volume ratio of the straw supernatant to illegal cooking oil being 35: 1-40: 1 is prepared. In a sequencing batch reactor (SBR), Halomonas sp.ZY-1 is subjected to aerobic culture with the unsterilized straw supernatant and illegal cooking oil MS culture medium to obtain thalli, and intracellular polyester substances are extracted.Polyester substances are further modified to obtain the mulching film with the tensile strength of 16-18 MPa, the mulching film is deeply buried in cultivated land soil with the depth of 20-30 cm, the 35d weight reduction rate is 40%-50%, and the 35d weight reduction rate is 0.3%-0.5% when the mulching film is placed in dry air. Under the condition that halophilic bacteria are not sterilized, wastes are utilized to synthesize the biological mulching film, so that the production cost is reduced to the maximum extent, and technical support is provided for realizing green ecological cycle and agricultural sustainable development.

The invention relates to a method for performing raw soil greening on moderate saline land through a soil conditioner comprising rice bran. The method comprises the following steps of selecting the moderate saline land, preparing the soil conditioner comprising the rice bran, applying the soil conditioner comprising the rice bran, and planting green grass after soil improvement. The soil conditioner comprising the rice bran comprises the following components in weight part: 21-23 parts of fermented rabbit manure, 12-14 parts of fermented pigeon manure, 5-7 parts of river sand, 3-5 parts of fly ash, 2-4 parts of zeolite powder, 1-3 parts of rice bran, 1-3 parts of caproic acid, 1-3 parts of amino acid salt, 3-5 parts of disodium hydrogen phosphate, 3-5 parts of sodium phosphate, 3-5 parts of sodium pyrophosphate, 3-5 parts of potash, 0.5-0.7 parts of diethyl aminoethyl hexanoate, 0.5-0.7 parts of forchlorfenuron, 0.5-0.7 parts of mulberry extract, 2-4 parts of microcrystalline cellulose, 0.5-0.7 parts of xanthate cellulose grafted acrylate, 0.1-0.3 parts of Halobacterium salinarium, 0.1-0.3 parts of bacillus megaterium and 0.1-0.3 parts of azotobacter vinelandii.

The invention discloses Bacillus atrophaeus E20303 and application thereof. The Bacillus atrophaeus E20303 has been preserved in Guangdong Microbial Culture Collection Center under the preservation number GDMCC No: 60695 since June 17, 2019. The Bacillus atrophaeus E20303 is capable of effectively inhibiting potato diseases caused by fusarium solani and potato virus Y. The Bacillus atrophaeus E20303 is a moderately halophilic bacterium which is isolated and purified from the water of Qarhan Salt Lake in Qinghai Province, and provides a new way for controlling potato diseases caused by fusariumsolani and potato virus Y; and moreover, the Bacillus atrophaeus E20303 is capable of effectively improving the problem of failure to achieve prevention and control effects caused by selective resistance of pathogens. Therefore, the Bacillus atrophaeus E20303 has good prospects of application.

The invention discloses a method for preparing gram-negative bacterium competent cells through outer membrane defects. The method comprises the following steps: knocking out and / or inhibiting genes related to outer membrane lipopolysaccharide (LPS) in a genome of gram-negative bacteria to obtain an outer membrane defect type strain; and preparing competent cells by utilizing the outer membrane defect type strain. The method provided by the invention can improve the conversion efficiency of wild-type strains (such as pseudomonas insecticola) which can be subjected to electrotransformation or chemical transformation. More importantly, for wild type gram-negative strains (such as halophilic bacteria and the like) which cannot be electrotransformed or chemically transformed, the competent cells prepared from the membrane-deficient strains can be electrotransformed or chemically transformed into DNA molecules, can be transformed into various plasmids, and express functional genes of the plasmids. The method plays an important role in gene editing of gram-negative bacteria.

The present invention discloses a mixed bacterial agent for reducing the dioxin content in domestic waste incineration tail gas. According to the present invention, a mixed bacterial amplification culture liquid and water are mixed according to a volume ratio of 1:50-100 to obtain the mixed bacterial agent, the mixed bacteria comprise Streptomyces cinereogriseus, Geotrichum candidum, Trichoderma koningii, Halobacterium halobium and Amycolatopsis sulphurea, wherein the preservation number of Streptomyces cinereogriseus is CGMCC 4.342, the preservation number of Geotrichum candidum is CGMCC 2.1135, the preservation number of Trichoderma koningii is CGMCC 3.2064, the preservation number of Halobacterium halobium is CGMCC 1.1952, and the preservation number of Amycolatopsis sulphurea is CGMCC 1.1144.

An SOS gene has been isolated through PCR cloning from Thellungiella halophila and is designated TSOS1. The polynucleotide encodes a transmembrane protein with similarities to plasma membrane Na+ / H+ antiporters from bacteria and fungi. The invention encompasses the TSOS1 gene, the corresponding protein, closely related polypeptides that confer salt tolerance, and related polynucleotides that encode a polypeptide that has Na+ / H+ antiporter activity.

The invention relates to a method for performing mild saline-alkali land raw soil greening through a biological modifier comprising fermented pig manure. The biological modifier comprising the fermented pig manure comprises the following components in parts by weight: 16-20 parts of peat, 8-12 parts of perlite, 4-8 parts of fermented sawdust, 1-3 parts of ammonium chloride, 1-3 parts of calcium hydrogen phosphate, 1-3 parts of magnesium sulfate, 0.3-1 part of acetic acid, 1-3 parts of amino acid salt, 8-12 parts of the fermented pig manure, 8-12 parts of eggplant straw powder, 0.3-1 part of forchlorfenuron, 0.3-1 part of carbaryl, 0.1-0.5 part of a danshen extract, 0.5-2 parts of sodium silicoaluminate, 0.2-0.5 part of cellulose xanthogenate grafted acrylate, 0.1-0.3 part of halobacterium salinarium, 0.1-0.3 part of Methanosarcinaceae, 0.1-0.3 part of bacillus subtilis, 0.1-0.3 part of Bacillus licheniformis, 0.1-0.3 part of bacillus laterosporus and 0.1-0.3 part of azospirillum.

The invention discloses a casing preservation process. According to the invention, salting preservation is adopted, a preservative solution is added into a special salt for casings, and then salting is conducted on the casings, wherein the mass of the preservative solution is 3-8% of the mass of the casings. The casing preservation process disclosed by the invention has the advantages that a casing preservative effectively inhibits the propagation of halophilic bacteria of the casings by utilizing the synergistic effect between lysozyme and an antioxidant, so the phenomenon of salty red of thecasings is prevented, the problem of salty red of the casings is fundamentally solved, the colors of the casings are protected, and the quality of the casings is better guaranteed.

The invention provides dunaliella salina-halophilic bacteria immobilized microspheres as well as a preparation method and application thereof, and belongs to the technical field of wastewater treatment. According to the preparation method, dunaliella salina and haloarcula are selected, a small amount of luminescent material is added to immobilize bacteria and algae on the basis of embedding with activated carbon and sodium alginate composite carrier, and when the immobilized dunaliella salina-halophilic bacteria are used for treating pickling wastewater, the separation of the bacteria and algae from the wastewater can be effectively realized, and has the advantages of stable treatment effect, secondary pollution prevention and the like.

The invention discloses a method for promoting halophilic activated sludge flora to secrete extracellular polymeric substances, belongs to the field of sewage treatment, and particularly relates to high-salinity wastewater biological treatment. Calcium and magnesium ions are added into a halophilic activated sludge system to increase the size of flocculating constituents of halophilic activated sludge and improve settling performance. Calcium and magnesium ion concentrated solution is added into the halophilic activated sludge system, biological selection pressure is applied to microorganismsin the system, and the microorganisms of Flavobacteriaceae in the halophilic sludge can rapidly multiply to become dominant species due to accumulation of the calcium ions. Flavobacteriaceae halophilic bacteria have high EPS (extracellular polymeric substance) secretion capacity, so that EPS of the halophilic activated sludge is improved. In addition, bridging functions among the calcium ions, themagnesium ions and the EPS can be enhanced, and connection among the extracellular polymeric substances and the microorganisms is promoted. The size of the flocculating constituents of the halophilicactivated sludge and the settling performance are greatly improved.

The invention discloses and provides a high-salinity wastewater treatment process utilizing halophilic bacteria YL5-2. The process employs the halophilic bacteria YL5-2 which is preserved in China General Microbiological Culture Collection Center and has the preservation number of CGMCC NO.16315, so that the strain YL5-2 used in the process is used as a new species of Halovibrio, which not only can degrade organic pollutants, but also can perform denitrification by taking organic matters as electron donors. The process is suitable for biochemical treatment of high-salinity wastewater within the salt concentration range of 3-32%, and is particularly suitable for treatment of high-salinity wastewater with the salt concentration of 10-25%.

The invention belongs to the technical field of microbial food processing, and particularly relates to oceanobacillus picturae and an application thereof. According to the invention, a halophilic bacterium strain is screened from Xiangyang brassica napobrassica brine, and the strain is identified as oceanobacillus picturae through morphology, physicochemistry and 16S rRNA sequence identification.The strain can significantly improve the variety and content of volatile substances in pickled vegetables, reduces the content of spicy substances, can improve the taste and flavor of pickled brassicanapobrassica, and has good development and application prospects.

The invention relates to the field of food detection, in particular to a kit for rapidly detecting vibrio parahaemolyticus in food and a detection method. The kit comprises a culture medium and detection liquid, and is characterized in that the culture medium is a halophilic bacteria selective agar culture medium containing 2% sodium chloride violet purple enrichmen, and the detection liquid is prepared from 5-10ml of surfactants, 2-5ml of alkaline solution, 2-5ml of acid solution, 5-8ml of protective solution, 20-50 microliters of DNA (deoxyribonucleic acid) extract solution, 1-5ml of 10*reaction buffer, 3-6ml of ethidium monoazide bromide, 0.35-0.4U / microliter BstDNA polymerase and 5-10 microliters of PCR (polymerase chain reaction) primers. The kit for detecting vibrio parahaemolyticus in the food is simple and convenient to operate without special equipment and instruments and short in detection time, and lower detection limit can reach 2cfu / g, so that requirements of the food safety detection departments on food safety supervision can be met.

The invention discloses a large-scale fermentation production method of extreme halophilic bacteria. An inclined plane of an isolation medium of a halophilic bacteria strain is used, the rotating speed of a shaking table is 100 revolutions per minute, the temperature is 35 DEG C, and the period is 8-20 days; a culture solution of a shake flask is inoculated to a 25L seeding tank with the amount of 5%, the temperature is 35 DEG C, the rotating speed is 100rpm, the ventilation capacity is 0.5v / v / m, the culturing period is 7-12 days, and a bacterial colony is red; the culture solution of the 25L seeding tank is inoculated to a 3000L fermentation tank with the amount of 1%, the temperature is 35 DEG C, the stirring speed is 180rpm, the ventilation capacity is 0.5v / v / m, and the fermentation period is 70-75 hours; a fermentation broth in the 3000L fermentation tank is inoculated to a large-scale production fermentation tank with the amount of 1%, the fermentation temperature is 35 DEG C, the stirring speed is 180rpm, the ventilation capacity is 0.5v / v / m, the period is 70-75 hours, the content of the halophilic bacteria reaches 108 / ml, and the effect of application of halophilic bacteria agent to hyper-saline oil deposit microbial enhanced oil recovery is remarkable.

The invention discloses a production method of a fresh-kept casing. The production method is characterized in that casing preservatives accounting for 2-10% of the mass of the casing are added into acasing salt in a casing pickling procedure before conduction of salting. The casing preservatives disclosed by the invention can effectively inhibit the growth and propagation of halophilic bacteria in the casing, and the casing preservatives are natural extracts which are stable in property and safe to use, so the quality of the casings is better guaranteed; three preservatives with preservativeand bacteriostatic functions are adopted, and the synergistic effect among preservatives is utilized to effectively inhibit the propagation of halophilic bacteria in the casings, thereby preventing the phenomenon of salty red of the casings and protecting the color of the casings; and the biological preservatives are low in cost, small in dosage and convenient to prepare, the red stain phenomenonin the storage and transportation processes of casings is reduced, the quality of the casings is protected, economic losses are reduced, and production benefits are increased. The biological preservatives for the casings are added in the salting and pickling stage of the casings, so the propagation of halophilic bacteria can be effectively inhibited; and the production method is simple in processand low in production cost.

The present invention relates to a crude oil degrading strain and its application, and the strain is classified and named as moderate halophilic bacteria ( Salinicola sp.) X4, the deposit number is CCTCC NO: M2018773, belonging to Gram-negative bacteria. The strain is opaque, beige, and the cells of the strain are short rods. Compared with the existing crude oil degrading strains, the strains provided by the invention have high degradation efficiency, simple cultivation, are suitable for high-salt environments, and have good application potential in the treatment of high-salt oil-containing wastewater in the petroleum industry and marine crude oil leakage accidents.

The invention discloses Bacillus shacheensis HNA-14<T> (=KCTC33145=DSM26902=CICC10559). Through separation, screening and physiological and biochemical identification of saline-alkali soil from Xinjiang yarkant, the bacterial strain is determined to be short rod gram-positive bacterium, which is strictly aerobic, generates spores, and has moveability, the cell size is (0.5-0.8)*(1.0-1.5)micron, and the bacterial colony is yellow. The NaCl tolerant range is 3%-20% (the optimal salinity is 7%-15%), the pH growth range is 6.5-10.0 (the optimal pH range is 7.5-8.0), and the temperature growth range is 5-40 DEG C (the optimal growth temperature is 25-30). The Xinjiang Bacillus shacheensis HNA-14<T> (=KCTC33145=DSM26902=CICC10559) is capable of effectively decomposing gelatin and starch, and has the enzyme activity of Esterase (C4), Esteraselipase (C8), Valinearylamidase, Acidphosphatase and Naphthol-AS-B1-phosphohydrolase; meanwhile, the bacterial strain has high salinity adaptability, and can survive at a high salinity. The moderately halophilic bacterium can be widely applied to production of secondary metabolite at a high salinity.