48 results about "Halobacterium salinarium" patented technology

Protein-based photovoltaic cells and the manufacture and use of protein-based photovoltaic cells are described. In one embodiment, bacteriorhodopsin from Halobacterium salinarum, which undergoes structural transitions when irradiated with a given wavelength of light, is used as the protein in the protein-based photovoltaic cells. In another embodiment, mutant bacteriorhodopsin from H. salinarum is used. Exposure of the protein to sunlight causes proton transfer across a membrane resulting in the generation of an electrical charge. The protein can be oriented and / or layered on a substrate and modified by mutation to enhance transmembrane proton transfer, covalent binding to a substrate and layering. The protein-based photovoltaic cells sequentially or simultaneously generate hydrogen gas from water or salt, which also can be harnessed to produce electricity.

The invention relates to a high-paraffin high-temperature high-salinity oil well microorganism paraffin removing and inhibiting agent and a preparation method thereof, and belongs to the technical field of tertiary oil recovery of an oil field. The microorganism paraffin removing and inhibiting agent is prepared from the following components in percentage by weight: 5 to 10 percent of halobacterium salinarium, 5 to 10 percent of hyperthermophilic archaeon, 8 to 15 percent of paraffin-thirsty bacteria, 20 to 25 percent of industrial brine, 5 to 10 percent of starch, 0.5 to 3 percent of peptone,0.5 to 3 percent of yeast extract and the balance of water. The preparation method of the microorganism paraffin removing and inhibiting agent comprises the following steps: adding the industrial brine, the starch, the peptone and the water into a fermentation tank, regulating pH (Potential of Hydrogen) value of a solution, rising the temperature to 40 to 55 DEG C, uniformly stirring, then heating to 125 DEG C, and sterilizing for 25 minutes; adding the yeast extract, the halobacterium salinarium, the hyperthermophilic archaeon and the paraffin-thirsty bacteria after cooling to 40 to 60 DEG C, fermenting for 48 hours by keeping the temperature be 55 to 60 DEG C, and cooling to room temperature, thus obtaining the microorganism paraffin removing and inhibiting agent. According to microorganism paraffin removing and inhibiting agent and the preparation method thereof, disclosed by the invention, the problems of low high-temperature high-salinity tolerance and poor paraffin removing andinhibiting effect of microorganism paraffin removing and inhibiting strains at present can be effectively solved.

The invention discloses a method for preparing gram-negative bacterium competent cells through outer membrane defects. The method comprises the following steps: knocking out and / or inhibiting genes related to outer membrane lipopolysaccharide (LPS) in a genome of gram-negative bacteria to obtain an outer membrane defect type strain; and preparing competent cells by utilizing the outer membrane defect type strain. The method provided by the invention can improve the conversion efficiency of wild-type strains (such as pseudomonas insecticola) which can be subjected to electrotransformation or chemical transformation. More importantly, for wild type gram-negative strains (such as halophilic bacteria and the like) which cannot be electrotransformed or chemically transformed, the competent cells prepared from the membrane-deficient strains can be electrotransformed or chemically transformed into DNA molecules, can be transformed into various plasmids, and express functional genes of the plasmids. The method plays an important role in gene editing of gram-negative bacteria.

An SOS gene has been isolated through PCR cloning from Thellungiella halophila and is designated TSOS1. The polynucleotide encodes a transmembrane protein with similarities to plasma membrane Na+ / H+ antiporters from bacteria and fungi. The invention encompasses the TSOS1 gene, the corresponding protein, closely related polypeptides that confer salt tolerance, and related polynucleotides that encode a polypeptide that has Na+ / H+ antiporter activity.

The invention discloses a method for promoting halophilic activated sludge flora to secrete extracellular polymeric substances, belongs to the field of sewage treatment, and particularly relates to high-salinity wastewater biological treatment. Calcium and magnesium ions are added into a halophilic activated sludge system to increase the size of flocculating constituents of halophilic activated sludge and improve settling performance. Calcium and magnesium ion concentrated solution is added into the halophilic activated sludge system, biological selection pressure is applied to microorganismsin the system, and the microorganisms of Flavobacteriaceae in the halophilic sludge can rapidly multiply to become dominant species due to accumulation of the calcium ions. Flavobacteriaceae halophilic bacteria have high EPS (extracellular polymeric substance) secretion capacity, so that EPS of the halophilic activated sludge is improved. In addition, bridging functions among the calcium ions, themagnesium ions and the EPS can be enhanced, and connection among the extracellular polymeric substances and the microorganisms is promoted. The size of the flocculating constituents of the halophilicactivated sludge and the settling performance are greatly improved.

The invention belongs to the technical field of microbial food processing, and particularly relates to oceanobacillus picturae and an application thereof. According to the invention, a halophilic bacterium strain is screened from Xiangyang brassica napobrassica brine, and the strain is identified as oceanobacillus picturae through morphology, physicochemistry and 16S rRNA sequence identification.The strain can significantly improve the variety and content of volatile substances in pickled vegetables, reduces the content of spicy substances, can improve the taste and flavor of pickled brassicanapobrassica, and has good development and application prospects.

The present invention relates to a crude oil degrading strain and its application, and the strain is classified and named as moderate halophilic bacteria ( Salinicola sp.) X4, the deposit number is CCTCC NO: M2018773, belonging to Gram-negative bacteria. The strain is opaque, beige, and the cells of the strain are short rods. Compared with the existing crude oil degrading strains, the strains provided by the invention have high degradation efficiency, simple cultivation, are suitable for high-salt environments, and have good application potential in the treatment of high-salt oil-containing wastewater in the petroleum industry and marine crude oil leakage accidents.

The invention discloses a composite type soil conditioner. The soil conditioner is prepared from components in parts by mass as follows: 30-40 parts of plant straw, 10-20 parts of fermented rabbit manure, 2-5 parts of fermented chicken manure, 2-5 parts of fermented cow dung, 5-10 parts of halobacterium salinarum, 5-10 parts of rice chaff, 2-5 parts of linseed oil residues, 1-3 parts of fly ash, 5-10 parts of polymerized amino acid, 1-3 parts of kaolin, 1-3 parts of crebrothecium ashbyii and 1-3 parts of compound bacteria. The composite type soil conditioner has the beneficial effects as follows: pH of raw soil with application of the conditioner is regulated, and the soil conditioner is convenient to use, environmentally friendly and high in biological waste degradation speed.