Dunaliella salina-halophilic bacteria immobilized microspheres as well as preparation method and application thereof

A technology of halophilic bacteria and microspheres, applied in biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, can solve the problem of poor permeability of bacteria and algal microspheres, affecting the growth and metabolism of microalgae, immobilized The problem of high global cost, to achieve good wastewater treatment effect, good denitrification and phosphorus removal effect, and the effect of promoting growth and reproduction

Pending Publication Date: 2021-07-23
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of immobilized microspheres is high, the immobilization on a single carrier is not easy to form, and the immobilization time is long
At the same time, there are still shortcomings such as poor permeability and poor physical and chemical properties of bacteria and algae microspheres, which affect the growth and metabolism of microalgae.

Method used

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  • Dunaliella salina-halophilic bacteria immobilized microspheres as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Under aseptic conditions, inoculate Dunaliella salina into the culture medium, and carry out alternating culture with a light-dark time ratio of 1:1 at a temperature of 25°C, with a light intensity of 4000Lux, and cultivate to the logarithmic growth stage That is, the algae liquid is obtained. The salina algae liquid cultured to the logarithmic phase was centrifuged at 4500r / min for 10min, washed with 10% NaCl solution, and centrifuged three times to remove inorganic salts and other substances attached to the algae cells. ℃ for later use.

[0029] Under aseptic operation conditions, inoculate the halophilic small box bacteria into the medium, and carry out alternating culture at a light-dark time ratio of 1:1 at a temperature of 25°C, with a light intensity of 3500Lux, and cultivate until the logarithmic growth period Yes, get the bacterial suspension. Take the bacterial suspension cultured to the logarithmic phase and centrifuge at 5000r / min for 10min, wash with ...

Embodiment 2

[0036] (1) Under aseptic conditions, inoculate Dunaliella salina into the culture medium, and carry out alternating culture with a light-dark time ratio of 1:1 at a temperature of 25°C, with a light intensity of 4000Lux, and cultivate to the logarithmic growth stage That is, the algae liquid is obtained. The salina algae liquid cultured to the logarithmic phase was centrifuged at 4500r / min for 10min, washed with 10% NaCl solution, and centrifuged three times to remove inorganic salts and other substances attached to the algae cells. ℃ for later use.

[0037] Under aseptic operation conditions, inoculate the halophilic small box bacteria into the medium, and carry out alternating culture at a light-dark time ratio of 1:1 at a temperature of 25°C, with a light intensity of 3500Lux, and cultivate until the logarithmic growth period Yes, get the bacterial suspension. Take the bacterial suspension cultured to the logarithmic phase and centrifuge at 5000r / min for 10min, wash with ...

Embodiment 3

[0044] (1) Under aseptic conditions, inoculate Dunaliella salina into the culture medium, and carry out alternating culture with a light-dark time ratio of 1:1 at a temperature of 25°C, with a light intensity of 4000Lux, and cultivate to the logarithmic growth stage That is, the algae liquid is obtained. The salina algae liquid cultured to the logarithmic phase was centrifuged at 4500r / min for 10min, washed with 10% NaCl solution, and centrifuged three times to remove inorganic salts and other substances attached to the algae cells. ℃ for later use.

[0045] Under aseptic operation conditions, inoculate the halophilic small box bacteria into the medium, and carry out alternating culture at a light-dark time ratio of 1:1 at a temperature of 25°C, with a light intensity of 3500Lux, and cultivate until the logarithmic growth period Yes, get the bacterial suspension. Take the bacterial suspension cultured to the logarithmic phase and centrifuge at 5000r / min for 10min, wash with ...

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Abstract

The invention provides dunaliella salina-halophilic bacteria immobilized microspheres as well as a preparation method and application thereof, and belongs to the technical field of wastewater treatment. According to the preparation method, dunaliella salina and haloarcula are selected, a small amount of luminescent material is added to immobilize bacteria and algae on the basis of embedding with activated carbon and sodium alginate composite carrier, and when the immobilized dunaliella salina-halophilic bacteria are used for treating pickling wastewater, the separation of the bacteria and algae from the wastewater can be effectively realized, and has the advantages of stable treatment effect, secondary pollution prevention and the like.

Description

technical field [0001] The invention belongs to the technical field of wastewater treatment, and in particular relates to an immobilized microsphere of salina-halophilic bacteria, a preparation method and application thereof. Background technique [0002] At present, the denitrification and phosphorus removal of high-salt wastewater mostly adopts the biological water treatment process, and most of the microorganisms used in it are non-halophilic microorganisms. These microorganisms will reduce the dehydrogenase activity under the action of salting out. Under normal circumstances, as the salinity increases, the cells will lose water, resulting in the separation of the cytoplasmic wall and the disintegration of microorganisms. The osmotic pressure caused by high salinity will increase the inhibitory effect on microorganisms, resulting in the inability of microorganisms to play a good role in the treatment of high-salt wastewater, and even lead to the final death of microorgani...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/32C02F3/34C12R1/01C12R1/89
CPCC02F3/325C02F3/34C12N11/04C12N11/10C12N11/14
Inventor 储金宇徐怡杜彦生
Owner JIANGSU UNIV
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