The invention provides cell cryopreservation fluid.The cell cryopreservation fluid is prepared from, by volume, 9-13% of dimethyl sulfoxide, 2-7% of human serum albumin, 1-3% of dextran, 1-5% of glucose and the balance solvent.Human serum albumin replaces animal serum to serve as a cell nutrient source, the pollution of animal source viruses is avoided, and nutrient substances of multiple components are effectively provided and can be directly absorbed and used by cells; in addition, a large amount of high-polymer protein is contained in human serum albumin and can form a hydration film, the number of crystals formed in the east village process is decreased, and mechanical loss and death of cells are reduced; by means of dextran, permeation pressure can be well maintained, the good permeation pressure environment can still be kept in the temperature decreasing process, and the cell death caused by temperature decreasing and permeation pressure changing is avoided, and the survival rate of cells recovering after being cryopreserved for a long time is increased.