Cell freezing medium

Abstract

The invention discloses a cell freezing medium. The cell freezing medium is prepared from the following components in percentage by volume and by concentration: 2 to 8 percent of dimethyl sulfoxide, 0.5 to 1.5 mg / ml of pawpaw extracting solution, 2.0 to 5mg / ml of chitosan quaternary ammonium salt, 0.5 to 2 percent of low molecular weight dextran, 8 to 15 percent of human serum albumin, 1.0 to 5mg / ml of glucan and the balance of DMEM culture medium. According to the cell freezing medium disclosed by the invention, the chitosan quaternary ammonium salt, the glucan and the pawpaw extracting solution are added, so that the limitation that chitosan can be only dissolved in a weak acid solution can be overcome; in addition, the effect of the cell freezing medium is better; by means of the glucan, the extracellular osmotic pressure can be improved and intracellular free water is reduced; due to the adoption of a permeable protective agent DMSO, cell rupture can be prevented, the melting pointof the intracellular water is reduced, and the formation of crystals is prevented; the permeable protective agent complements with a non-permeable protective agent glucan and the chitosan quaternaryammonium salt, so as to realize synergistic effects. The pawpaw extracting solution is a novel efficient natural antioxidant substance which is extracted from pawpaw and cannot be synthesized in a human body. The pawpaw extracting solution mainly plays an antioxidant role, and has the effects of improving cellular immune functions and prolonging cell activity.

Description

technical field [0001] The invention relates to the technical field of cell cryopreservation, in particular to a cell cryopreservation solution. Background technique [0002] Cell cryopreservation is one of the main methods of cell preservation. Using cryopreservation technology to store cells in -196°C liquid nitrogen at low temperature can temporarily remove the cells from the growth state and preserve their cell characteristics, so that the cells can be revived for experiments when needed. Moreover, moderately preserving a certain amount of cells can prevent the cells from being lost due to contamination of the cells being cultured or other accidents, which plays a role in cell preservation. In addition, some cells can also be purchased, donated, exchanged and shipped in the form of cell freezing. [0003] Cellular damage is normally caused when liquids freeze, either due to the formation of ice crystals and recrystallization within cells due to improper cooling and res...

AI Technical Summary

Problems solved by technology

[0005] At present, the existing cell cryopreservation solution cannot effectively preserve large-scale cultured cells, and the number of cells and cell viability after recovery from cryopreserved cells cannot meet the standards for clinical application. As a result, after large-scale culture of immune cells in vitro, it is necessary to application, otherwise it can only be discarded

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