Ralstonia solanacearum bacterial strain

A technology of Ralstia solanacearum and strains, applied in the field of microorganisms, can solve problems such as withering and wilting

Inactive Publication Date: 2012-03-14
福建省农业科学院农业生物资源研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to avoid wilting or dying of commercial crops caused by R. solanacearum infection, practitioners have been working on the research of R. solanacearum

Method used

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  • Ralstonia solanacearum bacterial strain
  • Ralstonia solanacearum bacterial strain
  • Ralstonia solanacearum bacterial strain

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Embodiment Construction

[0007] A non-pathogenic Ralstia solanacearum strain FJAT-1458 provided by the invention is obtained by separating and screening the stems of diseased tomato plants in Jian'ou City, Nanping City, Fujian Province.

[0008] 1. Isolation of strain FJAT-1458:

[0009] (1) Weigh 5 g of the stem portion of tomato at the early stage of the disease and rinse it with clear water, and cut the rinsed stem portion into small pieces of tissue; then, under aseptic conditions, treat the small piece of tissue with 75% alcohol for 30 seconds, Disinfect with 10% sodium hypochlorite for 8 minutes, rinse with sterile water for 3 times; then use a juicer to break up small pieces of tissue to obtain tissue fluid, and then draw 1mL of tissue fluid for gradient dilution, with a dilution of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 and 10 -7 ;

[0010] (2) Spread 200 μL of the diluted solution obtained in step (1) on a TTC medium plate, and then place the TTC medium plate at 30° C. for 2 days;

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Abstract

The invention relates to a ralstonia solanacearum bacterial strain. The bacterial strain is an avirulent ralstonia solanacearum bacterial strain FJAT-1458 and is preserved at China General Microbiological Center Culture Collection Center on August 4, 2011, and the preservation number is CGMCC No.5111. The ralstonia solanacearum bacterial strain has the advantages that the avirulent ralstonia solanacearum bacterial strain FJAT-1458 is provided, and the possibility is provided for studying and avoiding plant wilting or death phenomena caused by the pathogenicity of ralstonia solanacearum .

Description

【Technical field】 [0001] The invention relates to a microorganism, in particular to a non-pathogenic strain of Ralstia solanacearum. 【Background technique】 [0002] The existing Ralsotonia solanacearum is pathogenic and is a plant bacterial pathogen that can cause bacterial wilt of crops. It is widely distributed in tropical, subtropical and temperate regions, and it infects a wide range of hosts. Including cash crops such as peanuts, tomatoes, eggplants, and peppers, and bacterial wilt has continued to increase in China in recent years, and new hosts are constantly being discovered. Current research has found that Ralstia solanacearum can also infect canna and ginger flowers , yellow spike and zinnia 4 kinds of flowers. [0003] The pathogenic mechanism of R. solanacearum is complex. In the presence of host plant cells, R. solanacearum activates cell wall degrading enzymes, allergic reactions, and pathogenic genes encoded by type III secretion system components, Once it e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 车建美刘波林营志郑雪芳朱育菁唐建阳
Owner 福建省农业科学院农业生物资源研究所
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