Disease resisting and pest repelling biological seed coating inoculant and preparation method thereof

A biotype and coating technology is applied in the fields of biotype seed coating fungicide and its preparation, deworming and disease resistance, which can solve problems such as insufficient activity, and achieve the advantages of simple and easy preparation method, accelerated absorption, and prevention of erosion. Effect

Inactive Publication Date: 2017-06-20
山东金必来生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the existing seed coating agents generally have the problem of insufficient activity, and have obvious defects in preventing and controlling soil-borne diseases, promoting organic matter decomposition and emergence time, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this embodiment, the anti-disease, deworming, and biological seed coating bacterial agents are made of composite liquid bacteria and chitin stock solution; the weight ratio of the composite liquid bacteria and chitin stock solution is: composite liquid bacteria 70 parts; 10 parts of chitin stock solution;

[0027] The composite liquid strain mainly includes Pseudomonas fluorescens liquid strain and Bacillus thuringiensis liquid strain, and its weight proportion is: 55 parts of Pseudomonas fluorescens liquid strain; 15 parts of Pseudomonas fluorescens liquid strain share.

[0028] Its preparation method comprises the following steps:

[0029] Step 1. Preparation of culture medium

[0030] Take amino acid, potassium dihydrogen phosphate, glucose, peptone, urea and water to make a mixed solution with a weight content of 1.5% amino acid, 1.5% potassium dihydrogen phosphate, 0.5% glucose, 0.1% peptone, and 0.1% urea, with a pH value of 6.5~ 7.5;

[0031] Step 2, culti...

Embodiment 2

[0038] In this embodiment, the anti-disease, deworming, and biological seed coating bacterial agents are made of composite liquid bacteria and chitin stock solution; the weight ratio of the composite liquid bacteria and chitin stock solution is: composite liquid bacteria 80 parts; 20 parts of chitin stock solution;

[0039] Wherein, the composite liquid strains mainly include Pseudomonas fluorescens liquid strains and Bacillus thuringiensis liquid strains, and the weight ratio is: 60 parts of Pseudomonas fluorescens liquid strains; 20 parts of Pseudomonas fluorescens liquid strains share.

[0040] The preparation method comprises the following steps:

[0041] Step 1. Preparation of culture medium

[0042] Take amino acid, potassium dihydrogen phosphate, glucose, peptone, urea and water to make a mixed solution with a weight content of 2.0% amino acid, 2.0% potassium dihydrogen phosphate, 1.0% glucose, 0.5% peptone, and 0.5% urea, with a pH value of 6.5~ 7.5;

[0043] Step ...

Embodiment 3

[0050] In this embodiment, the anti-disease, deworming, and biological seed coating bacterial agents are made of composite liquid bacteria and chitin stock solution; the weight ratio of the composite liquid bacteria and chitin stock solution is: composite liquid bacteria 90 parts; 30 parts of chitin stock solution;

[0051]Wherein, the composite liquid strain mainly includes Pseudomonas fluorescens liquid strain and Bacillus thuringiensis liquid strain, and the weight ratio is: 65 parts of Pseudomonas fluorescens liquid strain; 25 parts of Pseudomonas fluorescens liquid strain share.

[0052] The preparation method comprises the following steps:

[0053] Step 1. Preparation of culture medium

[0054] Take amino acid, potassium dihydrogen phosphate, glucose, peptone, urea and water to make a mixed solution with a weight content of 2.5% amino acid, 2.5% potassium dihydrogen phosphate, 1.5% glucose, 0.9% peptone, and 0.9% urea, with a pH value of 6.5~ 7.5;

[0055] Step 2, cu...

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PUM

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Abstract

The invention discloses a disease resisting and pest repelling biological seed coating inoculant and a preparation method thereof. The seed coating inoculant is prepared from, by weight, 70-90 parts of a composite liquid strain and 10-30 parts of a chitin stock solution; and the composite liquid strain mainly comprises a Pseudomonas fluorescens liquid strain and a Bacillus thuringiensis liquid strain. Different microbes are combined to form strong thalli, the strains have different functions and are perfectly matched, the strains are individually cultured until the strains are mature and stable and then are combined, so the phenomenon of death of parts of strains, caused by the antagonism of various microbes which are commonly cultured, is avoided, soil borne diseases are controlled, the erosion of pathogens to seeds is prevented, the budding rate of the seeds is improved, the conversion of organic matters is promoted, nutrients are decomposed for seed absorption, and the seed coating inoculant well solves current seed processing and agricultural environmental protection problems.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a disease-resistant, insect-repellent, biological seed coating bacterial agent and a preparation method thereof. Background technique [0002] Seed coating agent (seed coating) can kill underground pests, prevent and control seed sclerotia seedling stage diseases, promote healthy growth and development of seedlings, improve crop varieties, increase seed germination rate and reduce seed usage, etc. It is generally made of pesticide raw materials, fertilizers, growth regulators, film-forming agents and supporting auxiliaries through a specific process. It can be directly or diluted and coated on the surface of seeds to form a certain strength and permeability. The pesticide formulation of the protective layer film. [0003] However, the existing seed coating agents generally have the problem of insufficient activity, and have obvious defects in preventing and controlli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N43/16A01P21/00A01P17/00A01C1/06C12N1/20C12R1/07C12R1/39
CPCA01N63/00A01C1/06A01N43/16C12N1/20A01N2300/00
Inventor 王晨裴德修付荣军
Owner 山东金必来生物科技有限公司
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