Halophilic bacteria fermentation production method applied to hyper-saline environment oil extraction

Abstract

The invention discloses a large-scale fermentation production method of extreme halophilic bacteria. An inclined plane of an isolation medium of a halophilic bacteria strain is used, the rotating speed of a shaking table is 100 revolutions per minute, the temperature is 35 DEG C, and the period is 8-20 days; a culture solution of a shake flask is inoculated to a 25L seeding tank with the amount of 5%, the temperature is 35 DEG C, the rotating speed is 100rpm, the ventilation capacity is 0.5v / v / m, the culturing period is 7-12 days, and a bacterial colony is red; the culture solution of the 25L seeding tank is inoculated to a 3000L fermentation tank with the amount of 1%, the temperature is 35 DEG C, the stirring speed is 180rpm, the ventilation capacity is 0.5v / v / m, and the fermentation period is 70-75 hours; a fermentation broth in the 3000L fermentation tank is inoculated to a large-scale production fermentation tank with the amount of 1%, the fermentation temperature is 35 DEG C, the stirring speed is 180rpm, the ventilation capacity is 0.5v / v / m, the period is 70-75 hours, the content of the halophilic bacteria reaches 108 / ml, and the effect of application of halophilic bacteria agent to hyper-saline oil deposit microbial enhanced oil recovery is remarkable.

Description

technical field [0001] The invention relates to the field of microorganisms and microbial fermentation. Specifically, the present invention relates to the technical field of a large-scale fermentation production method of extreme halophilic bacteria. Background technique [0002] In addition to the vast ocean (salt content 3.5-3.8%) on the earth, there are many salt ponds, salt lakes and saline-alkali lakes on the land. The salt content of the world-famous Dead Sea is as high as 31.5%. my country has a vast saline environment and rich resources of halophilic microorganisms. my country's Tibet, Qinghai, Xinjiang and Inner Mongolia have a large number of salt lakes and saline-alkali lakes with high salinity. The survey found that these salt areas have microbial growth, that is, the presence of halophilic bacteria. In the field of microbiology, halophilic bacteria usually refer to extreme halophilic bacteria, that is, microorganisms with an optimal salt concentration greate...

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Problems solved by technology

[0006] As mentioned above, the current research on extreme halophilic bacteria at home and abroad is focused on the research of its characteristic product rhodopsin and the construction of proprietary plasmids, and there are no related reports on the application of extreme halophilic bacteria to microbial oil recovery research.