Halophilic bacteria fermentation production method applied to hyper-saline environment oil extraction
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A production method, the technology of halophilic bacteria, applied in the production field of large-scale fermentation, can solve the problems that there is no research on microbial oil recovery of extreme halophilic bacteria
Inactive Publication Date: 2014-12-17
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0006] As mentioned above, the current research on extreme halophilic bacteria at home and abroad is focused on the research of its characteristic product rhodopsin
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0017] Example 1: A large-scale fermentation production method of extreme halophilic bacteria
[0018] A large-scale fermentation production method of extreme halophilic bacteria, the specific fermentation process steps are as follows:
[0019] 1. The halophilic bacteria strains are preserved on the slant of the separation medium, and cultivated in the shaker flask medium, the shaker speed is 100 rpm, the temperature is 35°C, and the time is 8-20 days. Cultivate until the liquid turns red or brown.
[0020] 2. Inoculate 5% of the shake flask culture medium into a 25L seed tank, the 25L medium is the same as the shake flask medium, at 35°C, 100rpm, ventilation rate 0.5v / v / m, culture time 7-12d, the colony is red.
[0021] 3. Inoculate 1% of the 25L seed tank culture medium into a 3000L fermenter. At 35°C, the stirring rate is 180rpm, the ventilation rate is 0.5v / v / m, and the fermentation time is 70-75h. Halophilic bacteria content reaches 10 8 pieces / ml.
[0022] 4. Inocula...
Embodiment 2
[0023] Embodiment two: the determination of fermentation process culture condition
[0024] The fermentation process of the seed shake flask during the scale-up fermentation of halophilic bacteria: 35°C, inoculate from a slope, shaker 100rpm, culture for 5 days, and the morphology of the bacteria is normal under the microscope. Used for inoculation of 25L seed tanks, 25L cultivation process: 35°C, 100rpm, ventilation rate 0.5v / v / m, cultivation time 5d, transferred to 3000L fermenter, 3000L fermentation process: 35°C, stirring rate 180rpm, ventilation rate 0.5v / v / m, the fermentation time is 70-75h, and the content of halophilic bacteria in the fermentation broth reaches 108 / ml.
[0025] Determination of the fermentation growth cycle,
[0026] 1. Through the statistics of three consecutive batches of fermentation results, the optimum fermentation time was determined. The result is as follows:
[0027] batch Amount of bacteria in fermentation broth (unit / ml) Ferm...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a large-scale fermentation production method of extreme halophilic bacteria. An inclined plane of an isolation medium of a halophilic bacteria strain is used, the rotating speed of a shaking table is 100 revolutions per minute, the temperature is 35 DEG C, and the period is 8-20 days; a culture solution of a shake flask is inoculated to a 25L seeding tank with the amount of 5%, the temperature is 35 DEG C, the rotating speed is 100rpm, the ventilation capacity is 0.5v/v/m, the culturing period is 7-12 days, and a bacterial colony is red; the culture solution of the 25L seeding tank is inoculated to a 3000L fermentation tank with the amount of 1%, the temperature is 35 DEG C, the stirring speed is 180rpm, the ventilation capacity is 0.5v/v/m, and the fermentation period is 70-75 hours; a fermentation broth in the 3000L fermentation tank is inoculated to a large-scale production fermentation tank with the amount of 1%, the fermentation temperature is 35 DEG C, the stirring speed is 180rpm, the ventilation capacity is 0.5v/v/m, the period is 70-75 hours, the content of the halophilic bacteria reaches 108/ml, and the effect of application of halophilic bacteria agent to hyper-saline oil deposit microbial enhanced oil recovery is remarkable.
Description
technical field [0001] The invention relates to the field of microorganisms and microbial fermentation. Specifically, the present invention relates to the technical field of a large-scale fermentation production method of extreme halophilic bacteria. Background technique [0002] In addition to the vast ocean (salt content 3.5-3.8%) on the earth, there are many salt ponds, salt lakes and saline-alkali lakes on the land. The salt content of the world-famous Dead Sea is as high as 31.5%. my country has a vast saline environment and rich resources of halophilic microorganisms. my country's Tibet, Qinghai, Xinjiang and Inner Mongolia have a large number of salt lakes and saline-alkali lakes with high salinity. The survey found that these salt areas have microbial growth, that is, the presence of halophilic bacteria. In the field of microbiology, halophilic bacteria usually refer to extreme halophilic bacteria, that is, microorganisms with an optimal salt concentration greate...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
IPC IPC(8): C12N1/20C12R1/01
Inventor 娄恺史应武
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
Login to view more 
Login to view more