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Bacillus shacheensis and application thereof

A technology of Bacillus serrata and bacillus, applied in bacteria, microorganisms, biochemical equipment and methods, etc., can solve problems such as little effect

Inactive Publication Date: 2014-12-24
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern industrial wastewater contains many difficult-to-treat chemical substances such as high-salt ions and toxic organic substances, and traditional biological methods often have little effect

Method used

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  • Bacillus shacheensis and application thereof
  • Bacillus shacheensis and application thereof
  • Bacillus shacheensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Screening and isolation of Bacillus shachei

[0024] Sample collection and strain screening and isolation

[0025] The samples were collected in Shache County, Xinjiang Uygur Autonomous Region. The samples come from multiple areas, distributed between 76°1'57" to 77°46'30" east longitude and 37°27'30" to 39°15" north latitude. The soil 5-10cm from the surface was collected in a sterile glass bottle, air-dried, ground, sieved, and stored at 4°C for subsequent separation. The screening medium was phenol-enriched medium (PT), humic acid-enriched medium (HVA), sodium molybdate-enriched medium (HNA) and high-salt-enriched medium (YCSS), and the purification medium was pancreatic Peptone Soy Broth (TSB+10% NaCl), strains were screened by inoculating different enrichment media. Add 1 g of the soil sample into a shaker flask filled with 25 ml of liquid medium, and culture it with shaking at 28°C for 7 days. Then draw the enrichment solution with a pipette gun, ...

Embodiment 2

[0036] Embodiment two: Bacillus shachei HNA-14 T = CICC 10559 phenotypic characteristics

[0037] by BIOLOG GEN The MicroStation automatic microbial identification system obtained the carbon source utilization of HNA-14 as shown in the table below. The reference strain of HNA-14 is Bacillus clausii DSM 8716 T

[0038] Carbon sources available: D-maltose, D-trehalose, D-cellobiose, gentiobiose, sucrose, D-turanose, stachyose, tetrazolium violet, D-raffinose, α-D - Lactose, β-methyl-D-glucoside, D-salicin, D-sorbitol, D-arabinol, inositol, pectin, D-gluconic acid, D-glucuronic acid, glucuronamide, L-lactic acid, D-malic acid, L-malic acid, lithium oxide, Tween-40, alpha-hydroxybutyric acid, alpha-hydroxybutyric acid, beta-hydroxybutyric acid, acetoacetic acid, propionic acid, acetic acid, butyric acid Sodium and sodium bromate; unavailable carbon sources: dextrin, vancomycin, tetrazolium blue, D-melibiose, N-acetyl-D-glucosamine, N-acetyl-β-D-mannosamine, N -Acet...

Embodiment 3

[0039] Embodiment three: Bacillus shachei HNA-14 T = CICC 10559 genome extraction and 16S rDNA sequencing

[0040] The total bacterial DNA was extracted by SDS method, the 16S rRNA gene sequence of the strain was amplified by PCR method, and the DNA sequencing was completed by Shanghai Sangon Bioengineering Technology Service Co., Ltd. Go to the GenBank (http: / / www.ncbi.nlm.nih.gov / ) database to submit the partial sequence of the 16S rRNA gene measured by each strain for registration to obtain the sequence number. Then perform BLAST comparison on NCBI (National Center for Biotechnology Information) to find the strain with the closest relationship. The sequences of the corresponding strains with the closest relatives were compared by BioEdit, and then the Neighbor-Joinin (N-J) method and the maximum likelihood method (ML method) were used to construct the phylogenetic tree on the software MEGA 5.0, and the bootstrap of the phylogenetic tree was 1000. The corresponding p...

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Abstract

The invention discloses Bacillus shacheensis HNA-14<T> (=KCTC33145=DSM26902=CICC10559). Through separation, screening and physiological and biochemical identification of saline-alkali soil from Xinjiang yarkant, the bacterial strain is determined to be short rod gram-positive bacterium, which is strictly aerobic, generates spores, and has moveability, the cell size is (0.5-0.8)*(1.0-1.5)micron, and the bacterial colony is yellow. The NaCl tolerant range is 3%-20% (the optimal salinity is 7%-15%), the pH growth range is 6.5-10.0 (the optimal pH range is 7.5-8.0), and the temperature growth range is 5-40 DEG C (the optimal growth temperature is 25-30). The Xinjiang Bacillus shacheensis HNA-14<T> (=KCTC33145=DSM26902=CICC10559) is capable of effectively decomposing gelatin and starch, and has the enzyme activity of Esterase (C4), Esteraselipase (C8), Valinearylamidase, Acidphosphatase and Naphthol-AS-B1-phosphohydrolase; meanwhile, the bacterial strain has high salinity adaptability, and can survive at a high salinity. The moderately halophilic bacterium can be widely applied to production of secondary metabolite at a high salinity.

Description

technical field [0001] The invention relates to the field of microbial strains and applications thereof, in particular to the technical field of a bacillus and its applications. Background technique [0002] Moderate halophilic bacteria is not a taxonomic term, but a general term for a class of extremophiles whose best salt-tolerant concentration is between 3% and 15%. It is widely distributed in various high-salt environments, such as oceans, salt lakes, saline-alkali lands, salt ponds, and many fermented foods. [0003] Due to their unique growth environment and salt-tolerant mechanism, moderately halophilic bacteria have many notable features: (1) they can grow at higher salt concentrations, reducing the risk of bacterial contamination during fermentation; (2) they do not require comparison Complex nutritional conditions, a wide range of carbon and nitrogen sources; (3) diverse physiological and biochemical characteristics, and great potential application value. [0004...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
Inventor 田永强刘波雷祖超
Owner SICHUAN UNIV
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