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Process for treating high-salinity wastewater by utilizing halophilic bacteria YL5-2

A high-salt wastewater treatment technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, water pollutants, etc., can solve the problem of not providing halophilic bacteria or halophilic bacteria species

Active Publication Date: 2020-07-07
BLUESTAR LEHIGH ENG INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the patent does not provide the types of halophilic bacteria or halophilic bacteria required for the treatment of high-salt wastewater

Method used

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  • Process for treating high-salinity wastewater by utilizing halophilic bacteria YL5-2
  • Process for treating high-salinity wastewater by utilizing halophilic bacteria YL5-2
  • Process for treating high-salinity wastewater by utilizing halophilic bacteria YL5-2

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The isolation and preservation of the halophilic bacteria YL5-2 of embodiment 1

[0027] The halophilic bacteria YL5-2YL5-2 was isolated from the sedimentary soil of Golmucharhan Salt Lake (36°51′N, 94°95′E) in Qinghai Province, China. The water of Chaerhan Salt Lake is saturated or close to saturated salt concentration all year round.

[0028] Prepare LB liquid medium with a NaCl concentration of 20%, add 250 mg / L glycerol, 250 mg / L glucose, and 50 mg / L methanol, and enrich and cultivate the sedimentary soil of Chaerhan Salt Lake at 30-35°C for 24-72 hours. The strains in the enriched culture medium were isolated by using YL solid medium. 1L medium contains the following components: glucose: 0.6g, trisodium citrate 0.5g, glycerol 2mL, yeast extract 0.8g, peptone 1.6g, dipotassium hydrogen phosphate 0.35g, potassium dihydrogen phosphate 0.1g, ammonium sulfate 0.25g, ammonium chloride 0.25g, MgSO 4 0.5g, CaCl 2 0.1g, NaCl 180g; trace element SL-4 10mL, pH 7.0-7.2; ...

Embodiment 2

[0030] Example 2 16S rRNA sequence analysis and whole gene sequence analysis of halophilic bacteria YL5-2

[0031] Strain YL5-2 genomic DNA was extracted using TaKaRa kit (TaKaRa MiniBEST Bacteria Genomic DNA Extraction 68Kit Ver.3.0).

[0032] 16S rRNA amplification was performed using universal bacterial primers 27F (5-AGAGTTTGATCMTGGCTCA G-3) and 1492R (5-TACGGYTACCTTGTTACGACTT-3). PCR sequencing was entrusted to Shanghai Sangon Biotechnology Co., Ltd. The complete 16S rRNA sequence of strain YL5-2 is 1518bp, and the GenBank accession number is MF782425.

[0033] The whole genome of strain YL5-2 was sequenced using the IlluminaMiSeq 2000 high-throughput sequencing platform of Shanghai Passino Biotechnology Co., Ltd. The original sequencing data was filtered and corrected using PRINSEQ (version number v 0.20.4) software, and then SOAP denovo software (version number v1.05) software with default parameters was used for genome base pairing, and then CheckM software (version ...

Embodiment 3

[0039] Example 3 Phenotypic characteristics and physiological and biochemical characteristics identification of halophilic bacteria YL5-2

[0040] Gram staining properties were tested using the BD Gram staining kit.

[0041] Cell motility was determined using half MA medium (0.5% agar, w / v).

[0042] Cell morphology was detected by transmission electron microscopy (TEM) analysis. That is, cells were picked from exponentially growing culture medium, stained with 0.5% uranyl acetate, and photographed under a microscope (Tecnai Spirit, FEI, Hillsboro, OR, USA).

[0043] Oxidase activity using the oxidase kit (bioMérieux), by adding 3.0% H 2 o 2 Catalase activity was determined by pouring the solution onto bacterial colonies and observing the generation of bubbles.

[0044] The temperature growth conditions were carried out on YL liquid agar medium, the temperatures were 4, 10, 15, 20, 25, 30, 33, 37, 40, 45 and 50 ° C, and the pH was constant at 7.5, and the strain YL5- 2 T...

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Abstract

The invention discloses and provides a high-salinity wastewater treatment process utilizing halophilic bacteria YL5-2. The process employs the halophilic bacteria YL5-2 which is preserved in China General Microbiological Culture Collection Center and has the preservation number of CGMCC NO.16315, so that the strain YL5-2 used in the process is used as a new species of Halovibrio, which not only can degrade organic pollutants, but also can perform denitrification by taking organic matters as electron donors. The process is suitable for biochemical treatment of high-salinity wastewater within the salt concentration range of 3-32%, and is particularly suitable for treatment of high-salinity wastewater with the salt concentration of 10-25%.

Description

technical field [0001] The invention belongs to the field of wastewater treatment and relates to a high-salt wastewater treatment process using halophilic bacteria YL5-2. Background technique [0002] High-salt wastewater treatment processes mainly include physical and chemical methods, biochemical methods and their combined processes. Physical and chemical methods include evaporation, membrane separation, ion exchange, advanced oxidation, electrolysis, etc. No matter how advanced these technologies are, the main idea of ​​the treatment is to separate the inorganic salts in the wastewater first, and then make the desalted or diluted wastewater reach the standard; therefore , its investment cost and operating cost are very high, and the evaporated mother liquor, solid salt, and reverse osmosis concentrate produced by desalination are solid waste or hazardous solid waste. [0003] At present, the biochemical treatment methods of high-salt wastewater mainly include: salinity a...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/30
CPCC02F3/34C02F2101/30
Inventor 徐军王开春李坤王强田凤蓉洪磊张璐璐孙文妮
Owner BLUESTAR LEHIGH ENG INST CO LTD
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