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Halophilic bacteria fungicide as well as preparation thereof, biological treatment system containing fungicide and application of fungicide in treating triazine wastewater

A technology of biological treatment and halophilic bacteria, applied in biological water/sewage treatment, methods based on microorganisms, microorganisms, etc., can solve problems such as complex components, and achieve the effects of simple operation, realization of recycling, and short process flow

Active Publication Date: 2014-06-18
SHANDONG WEIFANG RAINBOW CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the composition of atrazine wastewater from the pesticide factory is relatively complex and contains a relatively high concentration of salt (sodium chloride content above 6%), and general microorganisms cannot survive in a high-salt environment, so conventional non-halophilic strains and No single halophilic microbial strain can be well applied to the biological treatment of atrazine wastewater

Method used

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  • Halophilic bacteria fungicide as well as preparation thereof, biological treatment system containing fungicide and application of fungicide in treating triazine wastewater
  • Halophilic bacteria fungicide as well as preparation thereof, biological treatment system containing fungicide and application of fungicide in treating triazine wastewater
  • Halophilic bacteria fungicide as well as preparation thereof, biological treatment system containing fungicide and application of fungicide in treating triazine wastewater

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Embodiment 1

[0060] The strains screened by the present invention are mixed according to the ratio to obtain the halophilic bacterial bacterial agent required for degrading wastewater containing triazine compounds. The bacterial agent consists of: Salinicoccus roseus BJ-1CGMCCNO.8624 6 parts , Brevibacterium iodinum (Brevibacterium iodinum) BJ-2 CGMCCNO.8625 4 copies, Bacillus nitritophilus BJ-3 CGMCCNO.8626 8 copies, Halomonas alkalophilus ( Halomonas alkaliphila ) BJ-4 CGMCCNO.8627 6 copies.

[0061] The preparation method of the halophilic bacteria agent is as follows: inoculate 4 kinds of bacteria into the culture medium respectively, the inoculum amount is 10wt%, and expand the culture of the bacteria under the conditions of temperature 20°C and dissolved oxygen 2-4mg / L , the culture time is 72-96h. The medium used is glucose 9g / L, urea 2.4g / L, potassium dihydrogen phosphate 0.86g / L, sodium chloride 8wt%, pH 6.8-7.5. After culturing, each culture medium is centrifuged to obtain wet...

Embodiment 2

[0063] Mix the bacterial strains screened by the present invention according to the ratio to obtain the halophilic bacterial bacterial agent required for degrading the wastewater containing triazine compounds. The bacterial agent consists of: Salinicoccus roseus BJ-1 CGMCCNO.8624 8 Parts, Brevibacterium iodinum (Brevibacterium iodinum) BJ-2 CGMCCNO.8625 7 parts, Bacillus nitritophilus BJ-3 CGMCCNO.8626 6 parts, Halomonas alkalophilus ( Halomonas alkaliphila ) BJ-4 CGMCCNO.8627 10 parts.

[0064] The preparation method of the halophilic bacteria agent is as follows: inoculate 4 kinds of bacteria into the culture medium respectively, the inoculum amount is 10wt%, and expand the culture of the bacteria under the conditions of temperature 30°C and dissolved oxygen 2-4mg / L , the culture time is 72-96h. The medium used is glucose 10g / L, urea 2.2g / L, potassium dihydrogen phosphate 0.88g / L, sodium chloride 12wt%, pH 6.8-7.5. After culturing, each culture medium is centrifuged to ob...

Embodiment 3

[0066] The strains screened by the present invention are mixed according to the ratio to obtain the halophilic bacterial agent required for degrading wastewater containing triazine compounds. The bacterial agent consists of: Salinicoccus roseus BJ-1 CGMCCNO.8624 10 Parts, 10 parts of Brevibacterium iodinum BJ-2 CGMCCNO.8625, 6 parts of Bacillus nitritophilus BJ-3 CGMCCNO.8626, Halomonas alkalophilus ( Halomonas alkaliphila ) BJ-4 CGMCCNO.8627 10 parts.

[0067]The preparation method of the halophilic bacteria agent is as follows: inoculate 4 kinds of bacteria into the culture medium respectively, the inoculation amount is 10wt%, and expand the culture of the bacteria under the conditions of temperature 35°C and dissolved oxygen 2-4mg / L , the culture time is 72-96h. The medium used is glucose 11g / L, urea 2.0g / L, potassium dihydrogen phosphate 0.90g / L, sodium chloride 15wt%, pH 6.8-7.5. After culturing, each culture medium is centrifuged to obtain wet bacteria, and the wet ba...

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Abstract

The invention discloses a halophilic bacteria fungicide as well as preparation thereof, a biological treatment system containing the fungicide and an application of the fungicide in treating triazine wastewater. The halophilic bacteria fungicide is prepared by separating and screening degrading bacteria of four triazine compounds, and preparing according to a certain proportion, wherein the degrading bacteria include (Salinicoccus roseus )BJ-1, (Brevibacterium iodinum)BJ-2, Bacillusnitritophilus BJ-3, (Halomonasalkaliphila)BJ-4. The fungicide disclosed by the invention has stronger ability of degradation on the triazine wastewater and can be adaptive to a high-salt environment, has a very good degradation effect under conditions with salt content of 1%-15% and atrazine concentration less than 500mg / l, has obvious advantages in performances of degrading the triazine compounds compared with the traditional active sludge method, and has a degradation effect of over 98% for triazine compounds in the wastewater.

Description

technical field [0001] The invention relates to a halophilic bacterial agent, a preparation method of the bacterial agent, a biological treatment system containing the bacterial agent, and the use of the bacterial agent in treating high-salt wastewater containing triazine compounds, especially atrazine The invention belongs to the technical field of microorganism and wastewater treatment. Background technique [0002] Atrazine (Atrazine), also known as atrazine, the chemical name is ethyl promethazine (2-chloro-4-ethylamino-6-isopropylamino-1, 3, 5-triazine), is A widely used triazine herbicide, which belongs to weak polar compounds, was successfully developed by Ciba-Geigy Chemical Company in Switzerland in 1952. Atrazine targets a protein (QB) with a molecular weight of 32 kD on the thylakoid membrane of the chloroplast, and is widely used in corn, sorghum, pineapple and other farmlands to control most broad-leaved weeds and a few Grass weeds have a long half-life in soi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/30C02F3/34C12R1/01C12R1/13C12R1/07
Inventor 孙国庆侯永生李志清凌晓光窦道华何强王培宁周婧时成义孙继军刘后文尹海霞
Owner SHANDONG WEIFANG RAINBOW CHEM
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