Cheap synthesis of biodegradable mulching film from straws and illegal cooking oil by using halomonas sp ZY-1

A technology for degrading mulch and halomonas, applied in the field of agricultural materials, can solve the problems of white pollution, petrochemical plastic consumption of petrochemical energy, etc.

Pending Publication Date: 2020-07-07
NORTHEAST FORESTRY UNIVERSITY
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  • Description
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AI Technical Summary

Problems solved by technology

[0004] Aiming at a series of problems such as petrochemical plastics consume petrochemical energy and cause white pollution, the present invention proposes a production method of Halomonas sp. ZY-1 using straw and gutter oil to synthesize biodegradable plastic film under open conditions in a continuous batch reactor (SBR). Further optimize its material properties to make a biodegradable mulch film that meets the requirements of elasticity and light transmittance

Method used

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  • Cheap synthesis of biodegradable mulching film from straws and illegal cooking oil by using halomonas sp ZY-1
  • Cheap synthesis of biodegradable mulching film from straws and illegal cooking oil by using halomonas sp ZY-1
  • Cheap synthesis of biodegradable mulching film from straws and illegal cooking oil by using halomonas sp ZY-1

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the natural degradation situation of biodegradable plastic film

[0027] In order to simulate the degradation of biodegradable mulch film under natural conditions, this study used the weight loss rate of biodegradable mulch film buried in the soil to investigate its degradation ability.

[0028] (1) Take 3 prepared biodegradable mulch samples, each 20-30 mg, and record it as the initial mass M 0 .

[0029] (2) Place biodegradable plastic film samples in 20-25°C environment, 15-20cm deep cultivated land, take out the samples every 7 days to remove the surface soil, and place them in an oven at 40-55°C for drying treatment Weigh and record as Mn.

[0030] (3) The weight loss rate of biodegradable plastic film is calculated as follows:

[0031]

[0032] Degradation as figure 2 , in dry air, the 35d weight loss rate is 0.5%. At 20-25°C, the degradable plastic film is deeply buried in 15-20cm of cultivated soil, and the 35d weight loss rate reaches 40-5...

Embodiment 2

[0033] Embodiment 2, Halomonas sp.ZY-1 is not sterilized synthetic polyester class material in SBR reactor

[0034] (1) Pass the crushed straw through a 100-200 mesh fine sieve to obtain straw powder, soak 75-140g of straw powder per 1L of 1-2mol / L NaOH solution, treat with high temperature and high pressure for 2-4h, and centrifuge at 4000-6000r / min for 10min to collect the straw Supernatant, the main components are 2.5-6% cellulose, 15-30% xylose, 16-25% lignin, and 1-5% acid-soluble lignin;

[0035](2) MS medium components are: straw supernatant 750-800mL / L, waste oil 17-20mL / L, yeast extract 5-10g / L, Na 2 HPO 4 12H 2 O 10-15g / L, KH 2 PO 4 1-2g / L, NH 4 Cl 1-2g / L, MgSO 4 0.1-1.5g / L, Fe(III)-NH 4 -Citrate 0.05-0.1 g / L, CaCl 2 2H 2 O 0.02-0.1g / L, ZnSO 4 ·7H 2 O 0.1-0.5g / L, NaCI 60-70g / L, pH adjusted to 9.5-11, directly used to cultivate Halomonas sp.ZY-1 without sterilization;

[0036] (3) In an SBR reactor with a drainage ratio of 10 / 13, inoculate 1-5% of Halomo...

Embodiment 3

[0039] Example 3, Halomonas sp.ZY-1 uses rice straw and waste oil to synthesize polyester substances

[0040] According to the analysis results of the yield of polyesters in Example 2, the yield of polyesters synthesized from straws is low, and the film-forming properties of polyesters are poor. Add waste oil (composition 90-92% oleic acid, 3-4% triglyceride, 2-3% vegetable oil, 2-3% animal oil) for improvement, and use straw and waste oil as carbon sources to prepare MS medium.

[0041] (1) Straw and waste oil gradient MS medium configuration: 800mL / L straw supernatant, yeast extract 5g / L, Na 2 HPO 4 12H 2 O 10-15g / L, KH 2 PO 4 1-2g / L, NH 4 Cl 1-2g / L, MgSO 4 0.1-1.5g / L, Fe(III)-NH 4 -Citrate 0.05-0.1g / L, CaCl 2 2H 2 O 0.02-0.1g / L, ZnSO 4 ·7H 2 O 0.1-0.5g / L, waste oil is 5mL / L, 10mL / L, 15mL / L, 20mL / L, 25mL / L, 30ml / L;

[0042] (2) Put Halomonas sp.ZY-1 seed solution activated overnight in LB medium into MS medium with 1-5% inoculation amount, and cultivate 80- 8...

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Abstract

The invention discloses a method for synthesizing a biodegradable mulching film from straws and illegal cooking oil by using halomonas sp.ZY-1 under an open and non-sterile condition. Straws are treated to obtain a straw supernatant, and an MS culture medium with the volume ratio of the straw supernatant to illegal cooking oil being 35: 1-40: 1 is prepared. In a sequencing batch reactor (SBR), Halomonas sp.ZY-1 is subjected to aerobic culture with the unsterilized straw supernatant and illegal cooking oil MS culture medium to obtain thalli, and intracellular polyester substances are extracted.Polyester substances are further modified to obtain the mulching film with the tensile strength of 16-18 MPa, the mulching film is deeply buried in cultivated land soil with the depth of 20-30 cm, the 35d weight reduction rate is 40%-50%, and the 35d weight reduction rate is 0.3%-0.5% when the mulching film is placed in dry air. Under the condition that halophilic bacteria are not sterilized, wastes are utilized to synthesize the biological mulching film, so that the production cost is reduced to the maximum extent, and technical support is provided for realizing green ecological cycle and agricultural sustainable development.

Description

technical field [0001] The invention relates to a method for synthesizing a biodegradable plastic film cheaply by Halomonas sp. ZY-1 under non-sterile and open conditions by using straw and waste oil, and belongs to the field of agricultural materials. Background technique [0002] Drought and low temperature are one of the main obstacles to the sustainable development of agriculture in northern my country, seriously affecting the development of agricultural economy in Northeast China. The promotion of plastic film has greatly improved the local drought phenomenon, and also improved the production limitations of modern agriculture. Although plastic film mulching technology has greatly improved and enhanced the production of crops in arid areas of our country, it is also accompanied by many problems. The widely used plastic film is mainly made of petroleum-based materials, which has a stable chemical structure and cannot be degraded in a short time. And it will pollute the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L67/00C08L67/02C08K3/26C08K3/22C08J5/18C12N1/20A01G13/02C12R1/01
CPCC08J5/18C12N1/20A01G13/0275C08J2367/00C08J2467/02C08K2003/265C08K2003/2241Y02A40/28
Inventor 刘长莉刘成伟张杰赵敏徐启江
Owner NORTHEAST FORESTRY UNIVERSITY
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