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Fungicide composition for preventing and treating plant fungal diseases caused by alternaria alternata and preparation method and use of fungicide composition

A technology for plant fungal diseases and Alternaria, applied in botany equipment and methods, plant growth regulators, fungicides, etc., can solve the problem of drug resistance of pathogenic bacteria, the effect of drugs is greatly affected by environmental factors, colonization rules and The mechanism of action is not clear and other problems, to achieve the effect of inhibiting the growth of Alternaria, good insecticidal and bactericidal effects, and improving plant disease resistance

Pending Publication Date: 2019-12-03
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used chemical agents mainly include carbendazim, thiophanate-methyl, mancozeb, and chlorothalonil, etc., which can quickly and effectively inhibit Alternaria. Soil, air and other environmental pollution, destroying ecological balance
Biological control is a method to effectively prevent and control crop diseases by using beneficial microorganisms and microbial metabolites. It has the advantages of environmental protection and low toxicity. , the efficacy of the drug is greatly affected by environmental factors, and the use of technical requirements is strict, etc.
At present, there are still no reports of biopesticides that can effectively control various Alternaria fungal diseases for large-scale application.

Method used

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  • Fungicide composition for preventing and treating plant fungal diseases caused by alternaria alternata and preparation method and use of fungicide composition

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A fungicide composition for preventing and treating plant fungal diseases caused by Alternaria spp. is composed of the following materials: 20 parts of ginkgo endophytic Streptomyces lividans fermentation broth; 30 parts of ginkgo outer seed coat extract; and 1 part of Tween 80.

[0030] The preparation and application of a bacterial agent composition for preventing and controlling plant fungal diseases caused by Alternaria, comprising the following steps:

[0031] (1) Extraction of Ginkgo biloba exocarp extract: The collected ginkgo exocarp was placed in a ventilated indoor place to dry in the shade, and dried in a constant temperature drying oven at 60°C for 4 hours; 40 mesh sieve; extract the pulverized ginkgo exocarp powder and ethanol with a volume fraction of 70% at a solid-to-liquid ratio of 1:3 at 65°C for 3 times, 5h each time, and combine the crude extracts; use rotary evaporation The crude extract was concentrated under reduced pressure, and the ethanol solve...

Embodiment 2

[0036] A fungicide composition for preventing and treating plant fungal diseases caused by Alternaria, comprising the following materials: 20 parts of ginkgo endophytic Streptomyces lividans fermentation broth; 40 parts of ginkgo exocarp extract; and 2 parts of Tween 80.

[0037] The preparation and application of a bacterial agent composition for preventing and controlling plant fungal diseases caused by Alternaria, comprising the following steps:

[0038] (1) Extraction of Ginkgo biloba exocarp extract: The collected ginkgo exocarp was placed in a ventilated indoor place to dry in the shade, and dried in a constant temperature drying oven at 50°C for 3 hours; 40 mesh sieve; the pulverized ginkgo exotinum powder and ethanol with a volume fraction of 70% were refluxed for 3 times at 60°C at a solid-liquid ratio of 1:5, 5h each time, and the crude extracts were combined; The crude extract was concentrated under reduced pressure, and the ethanol solvent was evaporated to dryness...

Embodiment 3

[0043] A compound agent of fungal agents for preventing and treating fungal diseases of plants is composed of the following substances: 20 parts of fermentation broth of endophytic Streptomyces lividans from Ginkgo biloba; 50 parts of Ginkgo biloba outer seed coat extract; and 1 part of Tween 80. The preparation and application of the above-mentioned inoculum compound for preventing and controlling plant fungal diseases, comprises the following steps:

[0044] (1) Extraction of Ginkgo biloba exocarp extract: The collected Ginkgo biloba exocarp was placed in a ventilated indoor place to dry in the shade, and dried in a constant temperature drying oven at 50°C for 4 hours; 40 mesh sieve; the pulverized ginkgo exocarp powder and ethanol with a volume fraction of 70% were refluxed at 50°C for 3 times at a solid-liquid ratio of 1:7, 5h each time, and the crude extracts were combined; The crude extract was concentrated under reduced pressure, and the ethanol solvent was evaporated t...

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Abstract

The invention relates to the technical field of plant disease prevention and treatment, in particular to a fungicide composition for preventing and treating plant fungal diseases caused by alternariaalternata and a preparation method and use of the fungicide composition. The fungicide composition for preventing and treating the plant fungal diseases caused by the alternaria alternata is preparedfrom the components in parts by weight: 20 parts of endophytic streptomyces violascensfermentation liquid of ginkgo biloba, 30-50 parts of an exotesta extract of the ginkgo biloba and 1-2 parts of Tween 80, and the fungicide composition is used for preventing and treating the plant diseases caused by the alternaria alternata after being diluted with water for 100-150 times. The preparation methodof the fungicide composition comprises the steps of the extraction of the exotesta extract of the ginkgo biloba, the preparation of the endophytic streptomyces violascensfermentation liquid of the ginkgo biloba and the compounding of the endophytic streptomyces violascensfermentation liquid of the ginkgo biloba and the exotesta extract of the ginkgo biloba. According to the fungicide composition,the incidence and disease indexes of the plant diseases caused by the alternaria alternata can be effectively lowered, the relative inhibition rate is increased, the excellent prevention and treatmenteffect is achieved, and the advantages of stability, low toxicity, no residue, no environmental pollution and the like are achieved.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to a compound agent for preventing and controlling plant fungal diseases caused by Alternaria sp. and its preparation and use. Background technique [0002] Alternaria Nees is one of the most widely distributed deuteromycetes in the world. Some species of this genus can cause a variety of plant diseases, such as black spot, brown spot, stem blight, scab, etc. and so on, seriously endangering crop production. Its pathogenic mechanism is mainly mechanical penetration, secretion of degradative enzymes to destroy the cell wall of host cells, production of mycotoxins to act on host cells, and use of signal transduction pathways to mediate disease. [0003] At present, the control methods of Alternaria mainly include chemical control and biological control. Commonly used chemical agents mainly include carbendazim, thiophanate-methyl, mancozeb and chlorothalo...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01N63/00A01P3/00
CPCA01N63/00A01N65/00
Inventor 何福林袁志辉张斌刘小文张祖姣
Owner HUNAN UNIV OF SCI & ENG
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