Biological compound preparation for controlling plant virus disease and application thereof

A plant virus disease and preparation technology, applied in the direction of plant growth regulators, chemicals for biological control, application, etc., can solve the damage of plant growth and development, disrupt the normal metabolic activities of plant cells, reduce crop yield and Quality and other issues, to achieve the effect of prevention and control of plant virus diseases, good control effect, dosage and use cost reduction

Active Publication Date: 2012-09-26
CHINESE ACADEMY OF AGRI SCI LANGFANG PESTICIDE PILOT PLANT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After plant virus infects plants, the impact on plant cells is the same as that of animal viruses. It will disrupt the normal

Method used

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  • Biological compound preparation for controlling plant virus disease and application thereof
  • Biological compound preparation for controlling plant virus disease and application thereof
  • Biological compound preparation for controlling plant virus disease and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: The present invention is to the control effect test of tomato yellow leaf curl virus disease

[0014] Step 1, preparation of liquid medicine

[0015] Preparation of the mother solution: 10% Alternaria activator protein parent drug and 7.5% aminooligosaccharin parent drug were diluted with 0.1% Tween-80 aqueous solution respectively, and were prepared into 1000mg / L single-dose mother solution respectively, and then respectively Five groups of compound agent mother liquors are compounded with volume ratios of 1:1, 1:2, 2:1, 2:3, and 3:2 for future use.

[0016] Concentration setting: According to the activity of the medicine, the single-dose mother solution of Alternaria tennatus activating protein, the single-dose mother solution of amino-oligosaccharin, and the five groups of compound preparation mother solutions were respectively prepared with 0.1% Tween-80 aqueous solution to 10 mg / L , 20mg / L, 40mg / L, 80mg / L and 160mg / L 5 serial concentrations of liquid...

Embodiment 2

[0057] Embodiment 2: the control effect test of the present invention to rice stripe virus disease

[0058] This test procedure is basically the same with embodiment 1, and difference is:

[0059] The test target is rice stripe virus (Rice stripe virus, RSV), a viral pathogen transmitted by the small planthopper (Laodel ph x striatellus Fallén).

[0060] In step 3, inoculation and cultivation: inoculate with Hai'an colony (H colony) of the RSV transmission medium, and the inoculation age of rice is 0.5-1.5 leaf age.

[0061] Investigation: adopt the following grading method: 0, asymptomatic; 1, slight yellow-green mottled symptoms, diseased leaves are not curled, and plant growth is normal; 3, chlorosis on diseased leaves spreads and connects into irregular yellow-white or yellow-green Streaks, the diseased leaves are not curled or slightly curled, and the growth is basically normal; Grade 5, the diseased leaves are severely chlorotic, and the curls of the diseased leaves are...

Embodiment 3

[0068] Embodiment 3: the control effect test of the present invention to tobacco mosaic virus disease

[0069] This test procedure is basically the same with embodiment 1, and difference is:

[0070] The target of the test is the Tobacco mosaic virus (TMV) strain in China, and the poisonous tobacco leaf juice is purified before use.

[0071] In step 3, inoculation and cultivation: artificial rubbing is used for inoculation, the inoculation time is 24 hours, and the age of tobacco inoculation is 3 to 4 leaf ages.

[0072] Survey: The following grading method is used:

[0073] Grade 0: The whole plant is disease-free;

[0074] Grade 1: Heart leaves with bright veins or slightly mosaic leaves, no obvious dwarfing of plants;

[0075] Grade 3: 1 / 3 of the leaves are mosaic but not deformed, or the plants are dwarfed to more than 3 / 4 of the normal plant height;

[0076] Grade 5: 1 / 3-1 / 2 leaves are mosaic, or a few leaves are deformed, or the main veins are black, or the plants ar...

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Abstract

The invention relates to a biological compound preparation for controlling plant virus diseases and an application thereof. The purpose is to provide a biological medicament which can give full play to respective efficacy of alternaria tenuissima activator protein and amino-oligosaccharin so as to greatly improve the activity of the medicament and realize more effective control of the generation of plant virus diseases. The preparation of the invention is prepared by compounding alternaria tenuissima activator protein mother liquor with a concentration of 1000 mg/L and amino-oligosaccharin mother liquor with a concentration of 1000 mg/L according to a volume ratio of 1:(0.5-2.0); and when the volume ratio is 1:1, the compound preparation has the highest activity and the best control effect.

Description

technical field [0001] The invention relates to a biological preparation and its application, in particular to a biological compound preparation for preventing and treating plant virus diseases and its application. Background technique [0002] Viruses are a kind of extremely tiny obligate parasites without cell structure, and their morphology can only be observed with an electron microscope. The shape of plant virus is mostly spherical or rod-shaped. It is not a cell body, but a particle called a virion. This granule is composed of nucleic acid and protein. The nucleic acid is located in the center or axis, and the protein is surrounded by the nucleic acid. Viruses reproduce by self-replication (using various raw materials of host living cells), and the diseases caused by viruses infecting plants are called viral diseases. After plant virus infects plants, the impact on plant cells is the same as that of animal viruses. It will disrupt the normal metabolic activities of p...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N43/16A01P1/00
Inventor 邱德文曾洪梅杨秀芬郭立华袁京京
Owner CHINESE ACADEMY OF AGRI SCI LANGFANG PESTICIDE PILOT PLANT
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