Method for preparing ginseng fungus and ginseng fungus extract and application of ginseng fungus extract

A technology of fungi and ginseng, applied in the field of microorganisms, to achieve the effects of promoting improvement, promoting growth, promoting drought resistance and yield

Inactive Publication Date: 2011-06-08
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The endophyte mentioned in this patent, Neurospora panax, Neurospora panax, produces highly active plant growth-promoting substances, which have not been reported before.

Method used

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  • Method for preparing ginseng fungus and ginseng fungus extract and application of ginseng fungus extract
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  • Method for preparing ginseng fungus and ginseng fungus extract and application of ginseng fungus extract

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preparation example Construction

[0032] Preparation method of ginseng fungus and extract:

[0033] Preparation of ginseng endophytic fungi :

[0034] (1) Isolation of endophytic fungi from wild ginseng:

[0035] ①After the healthy wild ginseng sample is collected, process and wash it immediately, cut the root into small pieces, wash it with 75% alcohol, wash it with 10% pasteurized solution for 15-20 minutes, and finally wash it with sterile water for 3-5 minutes. Second-rate.

[0036] ② Cut the above-mentioned sterilized roots into 0.5cm-long sections under aseptic conditions and inoculate them on the Bengal red medium plate, and place them in a 28-degree incubator for 2-8 days, and the cut edges of the samples can be seen to grow. Mycelium, separated and purified repeatedly by plate streaking method and plate dilution method;

[0037] The above-mentioned sterilized roots were cut into 0.5cm long sections under aseptic conditions and inoculated on the Bengal red medium plate, and hyphae could be seen gr...

Embodiment 1

[0056] The preparation method of ginseng endophytic fungus is characterized in that: the steps are as follows:

[0057] (1) Isolation of ginseng endophytic fungi:

[0058] ① Handle and wash the healthy wild ginseng samples immediately after collection, then cut the root into small pieces, rinse with 75% alcohol, wash with 10% pasteurized solution for 15 minutes, and finally rinse with sterile water for 3 times;

[0059] ② Cut the above-mentioned sterilized roots into 0.5cm-long sections under aseptic conditions and inoculate them on the Bengal red medium plate, and place them in an incubator at 28°C for 2 days. Bacteria can be seen growing from the cut edges of the samples. Silk, separated and purified repeatedly by plate;

[0060] (2) Liquid fermentation culture of ginseng endophytic fungi:

[0061] Connect the fungus isolated in step "②" to the plate PDA medium, culture at 25°C for 5 days, use a puncher to dig agar block and inoculate it into a 250mL Erlenmeyer flask conta...

Embodiment 2

[0066] The preparation method of ginseng endophytic fungus is characterized in that: the steps are as follows:

[0067] (1) Isolation of ginseng endophytic fungi:

[0068] ① Handle and wash healthy wild ginseng samples immediately after collection, then cut the root into small pieces, rinse with 75% alcohol, wash with 10% pasteurized solution for 20 minutes, and finally rinse with sterile water 5 times;

[0069] ② Cut the above-mentioned sterilized roots into 0.5cm-long sections under aseptic conditions and inoculate them on the red Bengal medium plate, and place them in an incubator at 28°C for 8 days. Bacteria can be seen growing from the cut edges of the samples. Silk, separated and purified repeatedly by plate;

[0070] (2) Liquid fermentation culture of ginseng endophytic fungi:

[0071] Connect the fungus isolated in step "②" to the flat PDA medium, culture at 25°C for 7 days, use a puncher to dig out agar blocks and inoculate them into a 250mL Erlenmeyer flask contain...

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Abstract

The invention relates to the technical field of microorganisms, mainly relating to a hypha extract of a strain of endophyte separated from wild ginseng fibrous roots in the Changbai mountain under artificial culturing conditions and application of the hypha extract in prompting the growth of corns, improving the stress resistance and increasing the yield. The strain is preserved in China general microbiological culture collection center, and the category name of the fungus is Alternaria tenuissima. The invention aims to prompt the growth of the root system of corns, increase the utilization ratio of the fertilizer, improve the drought resistance and increase the yield of corns by the application of related products.

Description

[0001] 1. Technical field: [0002] The invention belongs to the technical field of microorganisms, and mainly relates to a preparation method and application of a ginseng fungus and an extract thereof, in particular to a preparation method and application of a wild ginseng endophytic fungus and an extract thereof. 2. Background technology: [0003] Plant endophytes are plant symbiotic bacteria with certain transformative properties. Many studies have shown that as a result of long-term co-evolution between endophytes and plants, they have formed a symbiotic form and mechanism of action that are mutually beneficial with symbiotic plants. Plant endophytes are a very large group. The complex evolutionary process has formed a variety of complex metabolic regulation mechanisms. Therefore, it is speculated that there are substances that can improve plant growth in plant endophytes. The traditional Big Five hormones are all found in different endophytes. With the development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/31C12P1/02A01N63/04C05F11/08
Inventor 陶承光杨涛杨镇胡文绣马晓颖王娜肖军王红肇莹陈珣龚娜
Owner LIAONING ACAD OF AGRI SCI
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