Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus

A kind of technology of Echinochloa japonicus and pathogenic fungi, applied in the direction of fungi, botanical equipment and methods, chemicals for biological control, etc., can solve the problems of unfavorable control and no effect, and achieve simple equipment, simple operation, The effect of strong stability

Inactive Publication Date: 2016-03-02
JINGGANGSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical control can produce better control effect in a short period of time, but because of the strong clonal reproduction characteristics of A. colloides, as long as there is one stem node, it can reproduce. Therefore, conventional mechanical weeding and plowing methods will only Cut it into more stem segments, which will lead to more new plants, which is not...

Method used

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  • Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus
  • Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus
  • Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The strong pathogenic bacteria strain J-14 with a diameter of 6 mm was transferred to potato agar medium (PDA), lotus leaf decoction medium (ADA), yeast extract powder peptone glucose agar medium (YPD), 1 %Polysorbate 80-corn agar medium (TCA), Sabouraud agar medium (SDLA), Chapeauer's medium (CDA) and Sabouraud dextrose agar medium (SDA) on 7 medium plates, placed at 25± Cultivate in the dark at 1°C in a biochemical incubator, measure the diameter of the colony after 5 days, then gently scrape off the spores with a glass slide, add an appropriate amount of sterile water to prepare a spore suspension, and count the spores with the hemocytometer method under a microscope output. Five replicates were set up for each treatment, and the results are shown in Table 1.

[0027] Table 1 shows that Alternaria is better on bacterium colony growth (P6 1, significantly higher than its sporulation production on other media (P<0.05), followed by 1% polysorbate 80-corn agar medium (T...

Embodiment 2

[0032] After dissolving the Alternaria toxin with a small amount of ethyl acetate (concentration: 2%), prepare 125, 250, 500, 1000, 2000 μg / mL toxin solutions with distilled water, respectively. The pathogenicity of the toxin to A. japonicus was detected by the needle-prick method of detached leaves. Select a healthy branch of A. japonicus without diseases and insect pests, take the third pair of leaves counted from the top down, rinse them with sterile water, absorb the water on the leaves with absorbent paper, and place them on a petri dish covered with a piece of wet filter paper. Gently puncture the epidermis of the leaves with a dissecting needle, drop 10 μL of the solution, and place it in a biochemical incubator at 25±1°C for dark cultivation. Measure the diameter of the lesion after 72 hours; at the same time, set the water control CK0 and 2% ethyl acetate Control CK1, each treatment was repeated 4 times, see the results figure 1 .

[0033] figure 1 It shows that Al...

Embodiment 3

[0035] After dissolving the Alternaria toxin with a small amount of ethyl acetate (concentration: 2%), a toxin solution with a concentration of 1000 μg / mL was prepared with distilled water. The pathogenicity of the toxins to the main symbiotic plants and common crops in the habitat of A. japonicus was detected by the acupuncture method of detached leaves. The treatment method of the leaves was the same as in Example 2, and then the grading statistics were carried out according to the diameter of the lesion. The pathogenicity grading standard is shown in Table 2, the statistical results are shown in Table 3.

[0036] Table 2 Pathogenicity level of Alternaria toxins to plants (acupuncture method of detached leaves)

[0037]

[0038] Table 3 shows that the toxin has strong pathogenicity to most symbiotic weeds of A. japonicus, but there are certain differences among different weeds. Among them, the toxin has the strongest pathogenicity to Imperata Imperata, Artemisia japonica...

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Abstract

The invention discloses a method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus. The method comprises following steps: preparation of a fluid nutrient medium; culturing of alternaria alternata; preparation of alternaria alternata toxin, and application of alternaria alternata toxin, wherein active toxin is prepared into a mixed solution with a concentration ranging from 125 to 2000<mu>g/ml, and the mixed solution is sprayed onto Alternanthera philoxeroides, so that obvious toxic symptoms of Alternanthera philoxeroides can be observed in three days, and weeds are killed. The alternaria alternata toxin possesses killing effect on most symbiotic weeds of Alternanthera philoxeroides. Operation of the preparation method of the liquid nutrient medium is simple; culture medium raw materials are easily available; needed equipment is simple; stability of the used toxin is high; extraction purification technology is simple; large-scale batch production can be realized; added accessory ingredients possess excellent synergistic effects on alternaria alternata toxin; the alternaria alternata toxin is applied via stem and leaf spraying; application is convenient; target weed Alternanthera philoxeroides can be killed effectively, and most symbiotic weeds can be killed at the same time; and environmental safety of the method is relatively high.

Description

technical field [0001] The invention relates to the technical field of the application of microorganisms to agricultural plant protection, in particular to a method for preventing and controlling alien weeds Asteroides japonicus by using pathogenic fungi. Background technique [0002] As one of the invasive alien weeds widely distributed in my country, it has strong vitality, rapid growth and reproduction, and rapid spread. It can invade a variety of habitats, endanger local biodiversity, cause crop production reduction, affect agricultural operations, and pollute water bodies. It blocks waterways, affects freshwater aquaculture and farmland irrigation, and develops into a vicious weed in many areas of our country. In 2003, it was included in the "China's first list of invasive alien organisms" announced by the State Environmental Protection Administration. The control of Alpinia chinensis mainly includes physical control, chemical control and biological control. Physical c...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P13/02C12N1/14
Inventor 周兵
Owner JINGGANGSHAN UNIVERSITY
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