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Huperzia serrata endophytic fungus resisting Botrytis cinerea and application of fungus

A technology of Huperzia serrata and endophytic fungi, applied in the field of microorganisms, can solve problems such as less research

Active Publication Date: 2019-10-25
湖北南章莆中药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the inhibition of Alternaria against Botrytis cinerea

Method used

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  • Huperzia serrata endophytic fungus resisting Botrytis cinerea and application of fungus
  • Huperzia serrata endophytic fungus resisting Botrytis cinerea and application of fungus
  • Huperzia serrata endophytic fungus resisting Botrytis cinerea and application of fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and Identification of Endophytic Fungus of Huperz serrata

[0020] 1. Isolation of endophytic fungi from Huperz serrata

[0021] Collected from Shizhu County, Chongqing (longitude 108.4585, latitude 30.3137, altitude 1427m), it was identified as wild Huperzia serrata by Professor Wang Keqin, an expert in pharmacognosy from Hubei Provincial Hospital of Traditional Chinese Medicine.

[0022] Use a brush to remove the dust and other attachments on the surface of wild Hupercia serrata, divide it into stems, leaves, and roots, add 75% alcohol solution and shake gently for 5 minutes. After filtering and drying, add 0.2% mercuric chloride to soak for 1.5 min, and wash with sterile water for 5 times. Wash water was collected as a control. Finally, the sterilized tissue samples were transferred to sterile filter paper to absorb moisture. Use sterile tweezers to gently pick up the sterilized tissues, place them gently on the surface of the PDA medium, place...

Embodiment 2

[0028] Example 2 Screening of endophytic fungus of Huperz serrata which is resistant to Botrytis cinerea

[0029] 1. Experimental materials and instruments

[0030] 1.1 Test strains

[0031] Tested strains: 50 endophytic fungal strains of Huperz serrata have been identified.

[0032] Pathogenic fungus for testing: Botrytis cinerea

[0033] 1.2 Experimental instrument consumables

[0034]High-speed refrigerated centrifuge (Thermo), percent balance (forerunner), constant temperature incubator (Shanghai Xinmiao Medical Instrument Manufacturing Co., Ltd.), camera (Canon), microscope (Guangdong Mingmei Optoelectronics Technology Co., Ltd.). Vernier caliper (Shanghai Minate Industrial Co., Ltd.), constant temperature culture shaker (Wuhan Ruihua Instrument Equipment Co., Ltd.), ultra-clean workbench (Beijing Donglian Haer Instrument Manufacturing Co., Ltd.).

[0035] 2. Experimental method

[0036] 2.1 Plate antagonistic strain screening

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Abstract

The invention discloses a Huperzia serrata endophytic fungus resisting Botrytis cinerea. The fungus is classified and named Alternaria alternata HR38-7, and is preserved in the China Center For Type Culture Collection, with the preservation number of CCTCC No. 2019388. The strain is screened from the Huperzia serrata endophytic fungus for the first time, and can be used for preventing and controlling the Botrytis cinerea of crops, and can be used as an active ingredient for preparing a biocontrol preparation for preventing and controlling the Botrytis cinerea of crops.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to an endophytic fungus of huperzia serrata which is resistant to gray mold, in particular to a strain of Alternaria alternate. Background technique [0002] In our country, due to the extensive use and non-standard use of pesticides, various ecological problems and food safety problems have been caused. Compared with chemical control, biological control has the characteristics of high safety and good environmental compatibility. At present, a variety of biological control agent products have been put into the market and applied to the disease control of field crops. Studies have shown that biological pesticides are safer than chemical pesticides. Liu Xinshe's research found that biological pesticides can improve the yield and quality of cucumbers, and compared with chemical pesticides, they can better protect food safety (Liu Xinshe 2019). Therefore, my country has increased its ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCC12R2001/645C12N1/145A01N63/30
Inventor 舒少华崔灵利严玲
Owner 湖北南章莆中药科技有限公司
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