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Plant endophytic bacterium SH-1 and application thereof

A plant endophytic bacteria, SH-1 technology, applied in plant growth regulators, applications, bacteria and other directions, can solve the problems of affecting the ecological environment, harming non-target microorganisms, unsafe human and livestock, etc., and achieving growth inhibition and significant control effects. Effect

Inactive Publication Date: 2014-01-01
YUNNAN TOBACCO CORP QUJING BRANCH +1
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  • Abstract
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  • Application Information

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Problems solved by technology

Long-term use of such chemicals can cause pathogens to develop drug resistance, resulting in poor control effects and even difficulty in controlling the harm and development of pathogens.
In addition, excessive use of chemical agents will cause pesticide residues and environmental pollution, which is extremely unsafe for humans and animals, and even directly endangers non-target microorganisms and directly affects the ecological environment.

Method used

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  • Plant endophytic bacterium SH-1 and application thereof

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preparation example Construction

[0022] 2. Preparation of biocontrol agents

[0023] The method of obtaining the biocontrol agent is a conventional method, as follows:

[0024] 1. Inoculate the SH-1 strain into LB culture medium and culture at 30°C with 200rpm shaking culture. After 12 hours, take samples in the ultra-clean workbench and measure the OD value at 600nm. During the measurement process, use the uninoculated culture medium to adjust to zero ;

[0025] 2. When the OD value is between 0.65 and 0.75, add the seed bacteria solution to the LB culture solution at a volume ratio of 1:100, and shake at 250 rpm at 30°C for 24 hours. Then centrifuge at 6000rpm for 10min, and obtain about 15g of bacteria per 1000mL culture solution, and each gram of bacteria contains 4×10 13 ~10 15 bacteria. Take the bacterium and the preservation solution according to 1g: 40ml to prepare the bacterial agent. The bacterial cell preservation solution was 0.5% Tween-80 saline.

[0026] The biocontrol agent is used to pre...

Embodiment approach

[0028] (1) SH-1 plate antagonism test

[0029] Select pathogenic bacteria of tobacco Alternaria Alternaria, Brassica oleracea, Tomato early blight. Pour 20 mL of PDA medium (glucose 20 g / L, peeled potato 200 g / L, agar 18 g / L, natural pH) into a 9 cm diameter petri dish. After cooling and solidifying, inoculate the pathogenic bacteria in the center of the PDA plate, inoculate SH-1 with a toothpick and place it evenly around 2cm from the center of the plate, and record and observe the diameter of the antibacterial zone after 72 hours.

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Abstract

The invention relates to a plant endophytic bacterium SH-1 and application thereof, belonging to the technical field of plant protection. The biocontrol strain Klebsiella variicola SH-1 was collected at China Center for Type Culture Collection on August 8, 2013; and the collection number is CCTCC NO: M2013367. The invention also relates to application of the plant endophytic bacterium SH-1 as a preparation for controlling tobacco brown spot, cabbage black spot and tomato early blight caused by alternaria strains. The plant endophytic bacterium SH-1 has the characteristics of being safe to humans, livestock and crops and being friendly to environment, has remarkable inhibition effect on growth of alternaria alternata (fries) keissler, alternaria oleracea M. Ibrath and alternaria solani, and has remarkable control effect on tobacco brown spot, cabbage black spot and tomato early blight caused by alternaria strains.

Description

Technical field: [0001] The invention relates to a plant endophytic bacterium SH-1 and its application, belonging to the technical field of plant protection. Background technique: [0002] Alternaria is one of the most widely distributed and economically important fungi in the world. More than 95% of the species are facultatively parasitic on plants, causing a variety of plant diseases, causing huge economic losses in the field and after production. It is one of the major plant diseases, such as tobacco red spot, tomato early blight, cabbage black spot, etc. Caused by fungi of the genus Alternaria. [0003] Tobacco red spot disease (Alternaria alternata (Fries) Keissler) is an important fungal disease on tobacco, which occurs in all tobacco regions. The disease has the characteristics of short incubation period and fast epidemic speed, and it mainly occurs in the late stage of field growth. , is one of the major diseases threatening tobacco leaf production everywhere. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/22
Inventor 李祖红曾嵘文国松赵明富林选知张瑞勤
Owner YUNNAN TOBACCO CORP QUJING BRANCH
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