A system for automatically completing the enrichment and cleaning of fat matrix components

Abstract

A system for automatically completing the enrichment and cleaning of fat matrix components, which includes a transmission path 1 from a buffer container to a fat processing structure, which is composed of an output pipeline 3, a main pipeline and an input pipeline 1; a transmission path 2 from the fat processing structure to a waste liquid collection container, which is composed of an output pipeline 1, a main pipeline and an input pipeline 3; a transmission path 3 from the fat processing structure to a centrifugal enrichment structure, which is composed of an output pipeline 1, a main pipeline and an input pipeline 2; a transmission path 4 from the centrifugal enrichment structure to a waste liquid collection container, which is composed of an output pipeline 2, a main pipeline and an input pipeline 3; and a transmission path 5 from a buffer container to a centrifugal enrichment structure, which is composed of an output pipeline 3, a main pipeline and an input pipeline 2. Each transmission path uses a power pump to provide energy transmission force at different times to ensure the completion of the processing after the autologous fat is extracted, and finally complete the enrichment and cleaning of fat matrix components.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and in particular relates to devices related to fat matrices, and specifically is a system for automatically enriching and cleaning fat matrices. Background Art

[0002] Adipose tissue matrix components (ADRCs) contain smooth muscle cells, stromal cells, endothelial cells, hematopoietic cells and other cells that are unique to adipose tissue and contribute to tissue repair. They also contain cells and non-cellular components that regulate immunity and fibrosis. Doctors and scientists have proven that the matrix components in adipose tissue have important functions such as promoting angiogenesis, regulating immunity, and resisting fibrosis. Adipose tissue can be obtained through simple liposuction surgery, which is a mature and minimally invasive procedure and can be used to repair tissue damage after surgery for many intractable diseases. Treatment using a mixture of these adipose matrix components and autologous fat can avoid the rejection and infection problems caused by artificial grafts, and help clinicians better solve major clinical problems that traditional grafts cannot solve.

[0003] Therefore, effectively and quickly obtaining autologous fat matrix components is a technical problem that needs to be solved clinically. Prior art 202211090467.0 pipeline system, fat and fat matrix component processing system, which discloses pipelines, but the enrichment of fat matrix components involves many pathways. How to effectively set up the pipeline to ensure the smooth progress of the pathway is a problem that needs to be solved in the extraction of fat matrix components.

[0004] In view of the above technical problems, the present invention provides a system for automatically completing the enrichment and cleaning of fat matrix components. Summary of the invention

[0005] At present, human autologous fat is usually used for processing, and the fat is split to obtain fat matrix components, and then the fat matrix components are transferred to the centrifugal chamber for enrichment of fat matrix components, and then the fat matrix cells are cleaned from the centrifugal chamber. Finally, the washed fat matrix components are used directly or mixed with fat tissue, and the fat matrix components can also be collected for standby. In this process, the buffer solution transmission path from the buffer solution holding container to the fat treatment structure during fat splitting; the waste liquid transmission path from the fat treatment structure to the waste liquid collection container after fat splitting; also includes the fat matrix transmission path for transporting the fat matrix components from the fat treatment structure to the centrifugal enrichment structure before fat matrix component enrichment; the waste liquid transmission path from the centrifugal enrichment structure to the waste liquid collection container after fat matrix component enrichment; the buffer solution transmission path from the buffer solution holding container to the centrifugal enrichment structure during fat matrix component cleaning, and the process involves multiple connections of each transmission path. The biggest feature of each path is that each path will not be connected at the same time, so it is necessary to design a system for enriching and cleaning fat matrices including pipelines, and the principle is to use the least pipeline combined with a power pump to complete the transmission of all paths. The specific technical solution is:

[0006] A system for automatically completing fat matrix component enrichment and cleaning, comprising

[0007] a fat processing structure connected to an output pipeline 1 for outputting liquid from the fat processing structure and an input pipeline 1 for inputting liquid into the fat processing structure;

[0008] a centrifugal enrichment structure, connected to a second output pipeline for outputting liquid from the centrifugal enrichment structure and a second input pipeline for inputting liquid into the centrifugal enrichment structure;

[0009] a buffer storage container connected to an output pipeline 3 for outputting liquid from the buffer storage container;

[0010] a waste liquid collection container connected to an input pipeline 3 for inputting waste liquid into the waste liquid collection container;

[0011] A main pipeline, the main pipeline is connected with input pipelines 1 to 3 and output pipelines 1 to 3 respectively; wherein input pipeline 3 and output pipeline 3 are connected with two edge positions in the upper and lower directions of the longitudinal axis of the main pipeline respectively; input pipeline 1, output pipeline 1, input pipeline 2 and output pipeline 2 are arranged between input pipeline 3 and output pipeline 3; wherein input pipeline 1 and output pipeline 1 are arranged on one side in the left-right direction of the longitudinal axis of the main pipeline, and input pipeline 2 and output pipeline 2 are arranged on the other side in the left-right direction of the longitudinal axis of the main pipeline;

[0012] A power pump is arranged outside the main pipeline, and is arranged between the first input pipeline and the first output pipeline, and is also arranged between the second input pipeline and the second output pipeline.

[0013] A stop valve is provided on each of the input pipelines 1 to 3 and the output pipelines 1 to 3.

[0014] The above-mentioned arrangement can ensure that the buffer solution transmission path from the buffer solution holding container to the fat processing structure; the waste liquid transmission path from the fat processing structure to the waste liquid collecting container; the fat matrix transmission path for the fat matrix component to be transported from the fat processing structure to the centrifugal enrichment structure; the waste liquid transmission path from the centrifugal enrichment structure to the waste liquid collecting container, and the buffer solution transmission path from the buffer solution holding container to the centrifugal enrichment structure, each transmission path can be constructed one by one through the main line and the input or output pipeline connected to the corresponding structure, and at the same time, the stop valves of the input and output pipelines corresponding to the required single transmission path are opened, and the stop valves on the input pipelines and output pipelines on other unnecessary transmission paths are closed, so that the constructed transmission path is powered by turning on the power pump to achieve liquid transmission in a single transmission path.

[0015] Furthermore, all the stop valves and power pumps are set on a setting platform; the platform is provided with guide marks for facilitating the setting of pipelines, and the guide marks are set between the stop valves, which include a main guide line and 6 branch guide lines corresponding to the pipelines. The pipelines are input pipeline 1 to input pipeline 3 and output pipeline 1 to output pipeline 13, totaling 6 lines.

[0016] Furthermore, at least one color guide structure is set on the main guide line; at least one color guide structure is set on the branch guide line, and a color matching structure is set on the main line corresponding to the main guide line and the branch pipeline corresponding to the branch guide line; the color guide structure is a color identification block or a color identification sticker set on the main branch guide line and the branch guide line, and the color matching structure is a color identification ring set on the main line and the branch pipeline, and the color identification ring is sleeved on the outside of the pipeline.

[0017] The beneficial effects of the present invention are:

[0018] Through effective pipeline layout and setting, according to the principle of time separation, only one power pump is set to provide power to the five transmission paths at different times, ensuring that the enrichment and cleaning of the fat matrix components are completed in chronological order. This setting method is reasonably designed and greatly reduces the space requirements for items required for the device.

[0019] By arranging the stop valve and the power pump on a setting platform, the setting complexity of each structure can be greatly reduced, and the setting of the pipeline is also simpler;

[0020] By setting guide marks on the setting platform and setting at least one color guide structure on the main guide line; setting at least one color guide structure on the branch guide line, and setting color matching structures on the main pipeline corresponding to the main guide line and the branch pipeline corresponding to the branch guide line, the difficulty of pipeline setting can be greatly avoided, the accuracy of pipeline setting can be ensured, and the efficiency of pipeline setting can be greatly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A schematic diagram of the planar structure of a system provided with a color matching structure according to the present invention;

[0022] Figure 2 A schematic diagram of a system of the present invention having one main pipeline and six branch pipelines, in which one transmission path is connected and the other paths are closed, wherein arrows indicate the direction of liquid transmission, stop valves without crosses are connected pipelines, and stop valves with crosses are closed pipelines;

[0023] Figure 3 A schematic diagram of a system of the present invention in which one main pipeline and six branch pipelines are arranged, and in which two transmission paths in the system are connected and other paths are closed;

[0024] Figure 4 A schematic diagram of a system of the present invention in which one main pipeline and six branch pipelines are arranged, and three transmission pathways in the system are connected and other pathways are closed;

[0025] Figure 5 A schematic diagram of a system of the present invention in which one main pipeline and six branch pipelines are arranged, and four transmission pathways in the system are connected and other pathways are closed;

[0026] Figure 6 A schematic diagram of a system of the present invention in which one main pipeline and six branch pipelines are provided, and five transmission pathways in the system are connected and the other pathways are closed;

[0027] Figure 7 A schematic diagram of the system structure plane in which one main pipeline, six branch pipelines and a pressure detection structure are arranged in the present invention;

[0028] Figure 8 The schematic diagram of the planar structure of the system structure of the present invention is provided with 1 main pipeline, 6 branch pipelines, a pressure detection structure and a liquid adding and pumping head;

[0029] Fig. 9 A schematic diagram of the planar structure of a guide mark arranged on a platform of the present invention;

[0030] Fig.10 This is a schematic diagram of the overall appearance of the centrifugal enrichment structure of the present invention;

[0031] Fig.11 It is a schematic diagram of the structure inside the centrifugal enrichment structure of the present invention;

[0032] Fig.12 This is a schematic diagram of the structure of the heating plate provided on the bottom of the cabin of the present invention;

[0033] Fig.13 It is a three-dimensional cross-sectional schematic diagram of a first embodiment of a collection cabin of a transverse enrichment structure of the present invention;

[0034] Fig.14 It is a three-dimensional cross-sectional schematic diagram of another embodiment of the collection chamber of the lateral enrichment structure of the present invention.

[0035] Description of main reference numerals

[0036] 1. Fat processing structure; 11. Output pipeline 1; 12. Input pipeline 1; 2. Centrifugal enrichment structure; 21. Output pipeline 2; 22. Input pipeline 2; 23. Centrifugal chamber; 24. Hatch cover; 25. Bunk bottom; 26. Temperature maintenance ring; 27. Longitudinal notch; 28. Heating plate; 210. Transverse enrichment structure body; 2101. Shell; 2102. Central cavity; 220. Collection chamber; 2201. Collection cavity; 2202. Sampling channel; 2203. Installation slot; 2204, interface; 2301, seal A; 2302, seal B; 240, sampler; 250, magnetic column; 3, buffer solution container; 31, output pipeline three; 4, waste liquid collection container; 41, input pipeline three; 5, main pipeline; 6, stop valve; 7, power pump; 8, pressure detection structure; 9, liquid adding and liquid extraction head; 10, setting platform; 101, main guide line; 102, branch guide line; 103, color guide structure; 104, color matching structure. DETAILED DESCRIPTION

[0037] refer to Figure 1-6 A system for automatically enriching and cleaning fat matrix components, comprising:

[0038] A fat processing structure 1, connected to an output pipeline 11 for outputting liquid from the fat processing structure 1 and an input pipeline 12 for inputting liquid into the fat processing structure 1;

[0039] A centrifugal enrichment structure 2, connected to an output pipeline 21 for outputting liquid from the centrifugal enrichment structure 2 and an input pipeline 22 for inputting liquid into the centrifugal enrichment structure 2;

[0040] a buffer container 3 connected to an output pipeline 31 for outputting liquid from the buffer container 3;

[0041] a waste liquid collection container 4, connected to an input pipeline 3 41 for inputting waste liquid into the waste liquid collection container 4;

[0042] A main pipeline 5, the main pipeline 5 is connected with input pipelines 12 to 3 and output pipelines 11 to 3 respectively; wherein the input pipeline 3 and the output pipeline 3 31 are respectively connected with two edge positions in the upper and lower directions of the longitudinal axis of the main pipeline 5; the input pipeline 1 12, the output pipeline 1 11, the input pipeline 2 22 and the output pipeline 2 21 are arranged between the input pipeline 3 and the output pipeline 3 31; wherein the input pipeline 1 12 and the output pipeline 1 11 are arranged on one side of the longitudinal axis of the main pipeline 5 in the left-right direction, and the input pipeline 2 22 and the output pipeline 2 21 are arranged on the other side of the longitudinal axis of the main pipeline 5 in the left-right direction;

[0043] A power pump 7 is arranged outside the main pipeline 5 and between the first input pipeline 12 and the first output pipeline 11 , and also between the second input pipeline 22 and the second output pipeline 21 .

[0044] A stop valve 6 is respectively arranged on the pipelines from the input pipeline 1 12 to the third-level input pipeline 1 12 to the third.

[0045] The transmission path 1 from the buffer storage container 3 to the fat processing structure 1 is constructed by the output pipeline 3 31, the main pipeline 5 and the input pipeline 1 12;

[0046] The transmission path 2 from the fat processing structure 1 to the waste liquid collection container 4 is formed by the output pipeline 1 11, the main pipeline 5 and the input pipeline 3;

[0047] The transmission path 3 from the fat processing structure 1 to the centrifugal enrichment structure 2 is constructed by the output pipeline 1 11, the main pipeline 5 and the input pipeline 2 22;

[0048] The transmission path 4 from the centrifugal enrichment structure 2 to the waste liquid collection container 4 is formed by the output pipeline 21, the main pipeline 5 and the input pipeline 3.

[0049] The transmission path five from the buffer storage container 3 to the centrifugal enrichment structure 2 is constructed by the output pipeline three 31, the main pipeline 5 and the input pipeline two 22.

[0050] Each transmission path can be constructed one by one through the main path 5 and the input or output pipeline connected to each corresponding structure, and at the same time, the stop valve 6 of the input and output pipeline corresponding to the required single transmission path is opened, and the stop valve 6 on the input and output pipeline of other unnecessary transmission paths is closed, so that the constructed transmission path is powered by turning on the power pump 7 to realize the liquid transmission in the single transmission path. The above method can well realize the utilization of 5 different transmission paths.

[0051] A more preferred implementation is: input pipeline 1 and input pipeline 2 are connected to main pipeline 5 at the same longitudinal axis position of main pipeline 5; output pipeline 1 and output pipeline 2 are connected to main pipeline 5 at the same longitudinal axis position of main pipeline 5; wherein input pipeline 1 and input pipeline 2 are closer to input pipeline 3; and output pipeline 1 and output pipeline 2 are closer to output pipeline 3. This arrangement ensures that the input pipeline is arranged on one side of the power pump 7 and the output pipeline is arranged on the other side of the power pump 7, ensuring that any input pipeline and output pipeline can be constructed into a required transmission path.

[0052] refer to Figure 1 , Figure 7 and Figure 8 A more preferred embodiment is as follows: two pressure detection tubes and / or a liquid adding and liquid extraction tube are connected and arranged at the edge positions of the main pipeline 5 in the vertical direction; a pressure detection valve is arranged outside the pressure detection tube; and a liquid adding and liquid extraction head 9 is arranged on the liquid adding or liquid extraction tube. The pressure detection in the pipeline is realized by arranging the pressure detection valve, and whether there is leakage is understood through the pressure detection; the liquid is added into the pipeline or the liquid is extracted from the pipeline by inserting the syringe into the liquid adding and liquid extraction head 9.

[0053] A more preferred implementation is as follows: the main pipeline 5 is provided with three sections of hoses, the output pipelines 11 to 3 and the input pipelines 12 to 3 are a complete section of hoses, the three sections of the main pipeline 5 are respectively a first section of the pipeline, a second section of the pipeline and a third section of the pipeline, wherein a two-way pipeline 1 is provided at the first end of the first section of the pipeline, and the first section of the pipeline is connected to the output pipeline 3 through the two-way pipeline 1; a four-way pipeline 1 is provided at the second end of the first section of the pipeline, and the first section of the pipeline, the second section of the pipeline, the input pipeline 1 and the input pipeline 2 are connected through the four-way pipeline 1; a four-way pipeline 2 is provided at the second end of the second section of the pipeline, and the second section of the pipeline, the third section of the pipeline, the output pipeline 1 and the output pipeline 2 are connected through the four-way pipeline 2; a two-way pipeline 2 is provided at the second end of the third section of the pipeline, and the two-way pipeline 2 connects the third section of the pipeline with the output pipeline 3 31; wherein the power pump 7 is provided outside the second section of the pipeline. The segmented setting can facilitate the assembly of pipelines and reduce the complexity of the process of integrated connection of soft materials. In addition, because the pipelines selected for fat matrix component enrichment and cleaning are made of special materials, if leakage occurs during the treatment of the same patient, a certain pipeline can be well handled to avoid the problem of all pipelines needing to be replaced due to a problem with one pipeline.

[0054] A more preferred implementation method is: replace two-way pipe one and two-way pipe two with three-way pipe one and three-way pipe two; add a pressure detection tube on the basis of each two-way pipe, and set a pressure detection valve on the outside of the pressure detection tube. By setting the pressure detection valve, the pressure in the pipeline can be detected, and the pressure detection can be used to understand whether there is any leakage.

[0055] A more preferred implementation is: replace the two-way pipeline one or the two-way pipeline two with the three-way pipeline one or the three-way pipeline two; add a liquid adding and liquid extraction tube on the basis of a two-way tube, and set a liquid adding and liquid extraction head 9 on the liquid adding or liquid extraction tube, and add liquid to the pipeline or extract liquid from the pipeline by inserting the syringe into the liquid adding and liquid extraction head 9.

[0056] A more preferred implementation is: replace the two-way pipeline one or two-way pipeline two with the four-way pipeline one or four-way pipeline two, and add a pressure detection tube and a liquid adding and liquid extraction tube to each four-way tube on the basis of a two-way tube; a pressure detection valve is arranged on the outside of the pressure detection tube; and a liquid adding and liquid extraction head 9 is arranged on the liquid adding or liquid extraction tube.

[0057] refer to Figure 1 and Fig. 9 A more preferred embodiment is that all the stop valves 6 and the power pump 7 are arranged on a setting platform 10; the platform is provided with guide marks for facilitating the setting of the pipelines, and the guide marks are arranged between the stop valves 6, and include a main guide line 101 and 6 branch guide lines 102 corresponding to the pipelines. By setting the guiding function of the main guide line 101 and the branch guide line 102, each pipeline can be well set to the accurate position and pass through each stop valve 6 to communicate with the corresponding instrument structure.

[0058] A more preferred implementation is: at least one color guide structure 103 is set on the main guide line 101; at least one color guide structure 103 is set on the branch guide line 102, and a color matching structure 104 is set on the main line 5 corresponding to the main guide line 101 and the branch line corresponding to the branch guide line 102; the color guide structure 103 is a color identification block or a color identification sticker set on the main branch guide line and the branch guide line 102, and the color matching structure 104 is a color identification ring set on the main line 5 and the branch line, and the color identification ring is sleeved on the outside of the line. By setting a color guide structure 103 of a main guide line 101 and a color guide structure 103 of a branch guide line 102, all pipelines can be quickly set to the correct position, avoiding the operator to repeatedly match according to the relationship between each pipeline and the connector. This setting method is simple to set and easy to match.

[0059] A more preferred implementation is: two color guide structures 103 are set on the main guide line 101, and two color guide structures 103 are set on the branch guide line 102, wherein the two color guide structures 103 on the main guide line 101 are set on the first section of the pipeline and the third section of the pipeline; the two color guide structures 103 on the branch guide line 102 are set on the branch guide line 102 corresponding to the output pipeline 21 and the input pipeline 22 connected to the centrifugal enrichment structure 2. The two color guide structures 103 on the main branch guide line are different in color; the two color guide structures 103 on the branch guide line 102 are different in color, and this method can improve the recognition efficiency, and the pipeline setting can be completed by finding a set of corresponding color guide structures 103 in the fastest way.

[0060] Alternatively, refer to Fig. 9 Two color guide structures 103 are arranged on the main guide line 101, and two color guide structures 103 are arranged on the branch guide line 102, wherein the two color guide structures 103 on the main guide line 101 are arranged on the first section pipeline and the third section pipeline; the two color guide structures 103 on the branch guide line 102 are arranged on the branch guide line 102 corresponding to the output pipeline 11 and the input pipeline 12 connected to the fat processing structure 1. The two color guide structures 103 on the main branch guide line are of different colors; the two color guide structures 103 on the branch guide line 102 are of different colors.

[0061] The method of providing the color guide structure 103 on the branch guide line 102 corresponding to the middle branch pipeline and on the first section pipeline and the third section pipeline at both ends of the main pipeline 5 can effectively improve the efficiency of recognition.

[0062] The fat processing structure 1 is a processing tank or a processing bag; preferably, the fat processing tank is arranged on a shaking weighing structure, the processing tank is shaken by the shaking weighing structure, and the processing tank is weighed after one step of processing is completed to ensure that a sufficient amount of fat is processed, thereby ensuring that the fat matrix components finally enriched are sufficient.

[0063] refer to Figure 10-12The centrifugal enrichment structure 2 is arranged in a centrifugal chamber with a cover, and the output pipeline 21 and the input pipeline 22 extend under the cover of the centrifugal chamber and onto the central axis pipeline of the centrifugal enrichment structure 2; the centrifugal enrichment device includes a transverse enrichment structure, and the central axis pipeline is arranged in the middle of the transverse enrichment structure; the rotation of the centrifugal enrichment structure 2 will not drive the rotation of the central axis pipeline, and this arrangement can ensure the position stability of the output pipeline 21 and the input pipeline 22 after being connected. The central axis pipeline is rotatably connected to the transverse enrichment structure, and a sealing material is arranged at the connection to maintain a leakproof seal; the specific implementation method of maintaining a leakproof seal includes that the sealing material is a material that does not deform with temperature; or, a temperature maintenance structure is arranged on the hatch cover 24, and the temperature maintenance structure is a temperature maintenance ring 26 extending from the center of the hatch cover 24 and surrounding the central axis pipeline. A longitudinal notch 27 is arranged on the temperature maintenance ring 26, which is used to connect the output pipeline 2 and the input pipeline 2. A heating structure is arranged in the temperature maintenance ring 26, and a temperature control structure is also arranged; preferably, the wire of the temperature maintenance structure is guided to the power supply position through the hatch cover 24; or, a heating plate 28 is arranged on the bottom of the cabin 25 to maintain the temperature of the entire centrifugal cabin 23. The heating plate 28 can be an annular plate arranged 360 degrees around the bottom of the cabin, and the heating plate 28 is connected to the temperature control structure. The above technical solution can well solve the leakage problem of the centrifugal enrichment device. In particular, the setting idea of ​​the heating plate 28 arranged at the bottom of the cabin can achieve the purpose by maintaining the temperature in the entire cabin. In addition, the constant temperature setting of the entire cabin can also effectively maintain the activity of the fat matrix components in the cabin. Maintain temperature in the range of 32-37°C.

[0064] refer to Fig.13 ; The transverse centrifugal structure includes a transverse enrichment structure body 210 and a collecting chamber 220; the transverse enrichment structure body 2101 has an outer shell and a central cavity 2102 inside the outer shell; the collecting chamber 220 is connected to both ends of the outer shell 2101, and the collecting chamber 2201 arranged inside the collecting chamber 220 is connected to the central cavity 2102; the collecting chamber 220 and the transverse enrichment structure body 2101 are integrally formed, and the collecting chamber 220 and the central cavity 2102 are always connected; a sampling channel 2202 is opened at the upper end of the collecting chamber 2201 and passes through the collecting chamber 220, and a sealing structure is arranged inside the sampling channel 2202; the sealing structure has two states of opening and closing; when the sealing structure is in the open state, the collecting chamber 2201 is connected to the outside world; when the sealing structure is in the closed state, the collecting chamber 2201 is cut off from the outside world.

[0065] Among them, reference Fig.13As shown, the sealing structure is an elastic seal A2301, and the seal A2301 is inserted into the sampling channel 2202; the seal A2301 and the sampling channel 2202 are interference-connected; the seal A2301 seals the sampling channel 2202 to prevent the target product from overflowing the collection chamber 2201 during the centrifugation process, and at the same time isolates the outside world from the collection chamber 2201 during the centrifugation process; only when the seal A2301 is inserted in the sampling channel 2202, it is in a closed state, and the collection chamber 2201 is cut off from the outside world.

[0066] Among them, reference Fig.13 As shown, a sampler 240 is inserted into the seal A2301, and the sampler 240 passes through the seal A2301 and is inserted into the bottom of the collecting chamber 2201; the sampler 240 is an elongated tubular structure; one end of the sampler 240 is inserted into the collecting chamber 2201, and contacts the fat cell matrix enriched in the collecting chamber 2201, and the fat cell matrix is ​​recovered or sampled through the negative pressure provided by the other end; after the sampler 240 is inserted into the seal A2301, the seal A2301 is in an open state, and the collecting chamber 2201 is connected to the outside.

[0067] Among them, reference Fig.14 The sealing structure is a spherical sealing member B2302; a mounting groove 2203 is provided in the middle of the sampling channel 2202, and one end of the mounting groove 2203 coincides with the sampling channel 2202; a sealing member B2302 whose outer surface is tightly fitted to the inner wall of the mounting groove 2203 is provided in the mounting groove 2203, and the sealing member B2302 rolls along the mounting groove 2203; the diameter of the sealing member B2302 is larger than the diameter of the sampling channel 2202; one end of the mounting groove 2203 coincides with the sampling channel 2202, and at the same time, the height of the other end of the mounting groove 2203 is lower than the height of the end that coincides with the sampling channel 2202, so that the sealing member B2302 is at the lower end of the mounting groove 2203 in a natural state, and at the same time, in a centrifugal state When the seal B2302 is in the state, the seal B2302 is subjected to the centrifugal force and slides from the end of the mounting groove 2203 with a lower height to the end overlapping with the sampling channel 2202; as above, when the seal B2302 is at the end of the mounting groove 2203 with a lower height, the sealing structure is in the open state, and the collection chamber 2201 is connected to the outside world. At this time, the sampler 240 can be used to conveniently remove the enriched fat cell matrix from the collection chamber 2201. When the seal B2302 is at the end where the mounting groove 2203 overlaps with the sampling channel 2202, the sealing structure is in the closed state, and the collection chamber 2201 is cut off from the outside world. At this time, it is in a centrifugal state, and the sampling channel 2202 is automatically blocked, which can effectively prevent the target product from overflowing from the collection chamber 2201.

[0068] Among them, reference Fig.14As shown, the other end of the mounting groove 2203 is fixedly connected with a magnetic column; the seal B2302 is ferromagnetic; in another embodiment different from the above, the height of the other end of the mounting groove 2203 can be equal to or higher than the height of the end where the mounting groove 2203 coincides with the sampling channel 2202, and the opening state of the seal B2302 is completed by the attraction of the magnetic column 250, and during centrifugation, the centrifugal force overcomes the attraction of the magnetic column 250 on the seal B2302. Compared with the above embodiment, control through the magnetic column 250 can be more stable, and is also suitable for the case where the sampling channel 2202 is reversed.

[0069] Among them, Fig.13 As shown, the bottom end of the collecting chamber 2201 is a spherical structure, and the height of the center of the sphere at the bottom end of the collecting chamber 2201 is lower than the height of the center of the cross-sectional circles at both ends of the central chamber 2102; the diameter of the central chamber 2102 gradually decreases from the center to both sides; after the fat cell matrix produced by centrifugation enters the collecting chamber 2201, it will be enriched at the bottom end of the collecting chamber 2201, and the height of the bottom end of the collecting chamber 2201 is lower than the height of both ends of the central chamber 2102, which can effectively prevent the target product from flowing back to the central chamber 2102 after the centrifugation is completed.

[0070] Among them, Fig.13 As shown, the bottom of the collecting chamber 2201 has a spherical center, and the center line of the sampling channel 2202 points to the spherical center of the collecting chamber 2201; when the sampler 2404 is inserted into the collecting chamber 2201 through the sampling channel 2202, it can point to the bottom of the collecting chamber 2201 to ensure thorough sampling. At the same time, an interface 2204 is provided on the top of the sampling channel 2202 for connecting to an external negative pressure source to sample or recover the target product in the collecting chamber 2201.

[0071] The buffer solution holding container 3 is a buffer solution holding bag suspended at a high place; it is suspended on the hanging rod of the setting platform 10; the waste liquid collecting container 4 is a waste liquid collecting bag suspended on the setting platform 10, and the overall structure is suspended below the setting platform 10; this setting can effectively reduce the working intensity of the power pump 7.

[0072] The power pump 7 is a peristaltic pump arranged on the setting platform 10. The setting of the peristaltic pump can ensure that the main pipeline 5 is clamped on the peristaltic pump, and the setting is simple and convenient. The setting will not affect the shape of the pipeline and no liquid flows through the peristaltic pump.

[0073] The buffer was sodium lactate Ringer's solution.

[0074] A process for removing fat from the human body during surgery and enriching and cleaning the fat matrix components;

[0075] S1: Use fat extraction equipment to extract fat from the human body for subsequent fat cleaning, fat splitting, fat matrix component enrichment and fat matrix component cleaning processes.

[0076] S2: Install the pipelines, set the fat processing structure 1, the centrifugal enrichment structure 2, the waste liquid collection container 4 and the buffer storage device to the appropriate positions, prepare the pipeline installation, adapt the color guide structure 103 on the main guide line 101 and the branch guide line 102 to the color matching structure 104 on the main pipeline 5 and the branch pipeline, and complete the placement of the pipelines; then set the main pipeline 5 to the peristaltic pump; set each branch pipeline to the corresponding stop valve 6, and set the pressure detection tube to the pressure detection valve; then connect each branch pipeline with the fat processing structure 1, the centrifugal enrichment structure 2, the waste liquid collection container 4 and the buffer storage device;

[0077] S3: Pipeline inspection: check whether there is leakage at the connection of each pipeline. If there is no leakage, proceed to the next step;

[0078] S4: The fat is sent into the fat processing structure 1, and is left to stand for separation. After separation, the waste liquid is transported to the waste liquid collection container 4 through the transmission path 2, and then the fat is weighed. When a sufficient amount of fat is reached, the next step is carried out.

[0079] S5: Cleaning of fat tissue, transferring the buffer solution from the buffer solution holding container 3 to the fat processing structure 1 through the transmission path 1 to clean the fat, and after cleaning, transferring the waste liquid to the waste liquid collection container 4 through the transmission path 2.

[0080] S6: Lysis of adipose tissue, adding a lysis solvent into the fat processing structure, and then transferring the buffer solution from the buffer solution container to the fat processing structure through a transmission path 1 to lyse the cleaned fat. After the lysis is completed, the processing tank will stand for five minutes for separation; the fat matrix component is separated; note that the lysis solvent added in this step is Celase or an equivalent reagent.

[0081] S7: Enrichment of fat matrix components. The separated fat matrix components are transferred from the fat processing structure 1 to the centrifugal chamber of the centrifugal enrichment device through the transmission path 3 for enrichment by centrifugation. After enrichment, the enriched waste liquid is transferred from the centrifugal enrichment structure 2 to the waste liquid collection container 4 through the transmission path 4.

[0082] S8: Cleaning of the fat matrix components. After the fat matrix components are enriched, the fat matrix components are cleaned three times in the centrifugal chamber. Before cleaning, the buffer solution is transferred from the buffer solution holding container 3 to the centrifugal chamber of the centrifugal enrichment structure 2 by using the transmission path 5. After each cleaning, the waste liquid is transferred from the centrifugal enrichment structure 2 to the waste liquid collection container 4 by using the transmission path 4 and collected after completion.

[0083] S9: Preparation of clinical fat tissue, re-extracting fat aspirate and injecting it into the cleaned fat processing structure 1, separating it, discharging waste liquid after separation, and then washing it again, discharging waste liquid after washing, and then injecting fat matrix components into the fat processing structure 1, oscillating and mixing fat and fat matrix components,

[0084] The cleaning of the extracted fat involved in step S9 and the fat matrix component cleaning process in step S8 are performed simultaneously in the fat processing structure and the centrifugal enrichment device respectively. This arrangement can greatly reduce the time for processing the fat and fat matrix components input into the human body.

[0085] The technical solutions in the embodiments of the present invention are clearly and completely described above through specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation methods. In the absence of conflict, the above embodiments and features in the embodiments can be combined with each other. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

Claims

1. A system for automatically completing the enrichment and cleaning of fat matrix components, comprising: a fat processing structure connected to the output pipeline 1 and the input pipeline 1; A centrifugal enrichment structure connected with output pipeline 2 and input pipeline 2; a buffer containing container connected to an output pipeline 3; a waste liquid collection container connected to an input pipeline 3; A main pipeline, the main pipeline is connected with input pipelines 1 to 3 and output pipelines 1 to 3 respectively; wherein input pipeline 3 and output pipeline 3 are connected with two edge positions in the upper and lower directions of the longitudinal axis of the main pipeline respectively; input pipeline 1, output pipeline 1, input pipeline 2 and output pipeline 2 are arranged between input pipeline 3 and output pipeline 3; wherein input pipeline 1 and output pipeline 1 are arranged on one side in the left-right direction of the longitudinal axis of the main pipeline, and input pipeline 2 and output pipeline 2 are arranged on the other side in the left-right direction of the longitudinal axis of the main pipeline; A power pump is arranged on the main pipeline, and three pipelines are arranged on each side of the power pump; A stop valve is provided on the pipelines from input pipeline 1 to input pipeline 3 and from output pipeline 1 to output pipeline 3 respectively.

2. The enrichment and cleaning system according to claim 1, characterized in that: It includes 5 transmission channels, namely: A transmission path 1 from the buffer storage container to the fat processing structure is constructed by an output pipeline 3, a main pipeline and an input pipeline 1; A transmission path 2 from the fat processing structure to the waste liquid collection container is composed of an output pipeline 1, a main pipeline and an input pipeline 3; A transmission path 3 for transporting fat from the fat processing structure to the centrifugal enrichment structure, which is composed of an output pipeline 1, a main pipeline and an input pipeline 2; A transmission path 4 from the centrifugal enrichment structure to the waste liquid collection container is composed of an output pipeline 2, a main pipeline and an input pipeline 3; The transmission passage five from the buffer storage container to the centrifugal enrichment structure is composed of the output pipeline three, the main pipeline and the input pipeline two.

3. The enrichment and cleaning system according to claim 2, characterized in that: Input pipeline 1 and input pipeline 2 are connected to the main pipeline at the same longitudinal axis position of the main pipeline; output pipeline 1 and output pipeline 2 are connected to the main pipeline at the same longitudinal axis position of the main pipeline; among them, input pipeline 1 and input pipeline 2 are closer to input pipeline 3; and output pipeline 1 and output pipeline 2 are closer to output pipeline 3.

4. The enrichment and cleaning system according to claim 3, characterized in that: Two pressure detection tubes and / or a liquid adding and extracting tube are connected and arranged at the edge positions in the vertical direction of the main pipeline; a pressure detection valve is arranged outside the pressure detection tube; and a liquid adding and extracting head is arranged on the liquid adding or extracting tube.

5. The enrichment and cleaning system according to claim 4, characterized in that: The main pipeline is provided with three sections of hoses, output pipelines one to three and input pipelines one to three are a complete section of hose, and the three sections of the main pipeline are respectively the first section of the pipeline, the second section of the pipeline and the third section of the pipeline, wherein the first end of the first section of the pipeline is provided with a two-way pipeline one, and the first section of the pipeline is connected with the output pipeline three through the two-way pipeline one; the second end of the first section of the pipeline is provided with a four-way pipeline one, and the first section of the pipeline, the second section of the pipeline, the input pipeline one and the input pipeline two are connected through the four-way pipeline one; the second end of the second section of the pipeline is provided with a four-way pipeline two, and the second section of the pipeline, the third section of the pipeline, the output pipeline one and the output pipeline two are connected through the four-way pipeline two; the second end of the third section of the pipeline is provided with a two-way pipeline two, and the two-way pipeline two realizes the connection between the third section of the pipeline and the output pipeline three; wherein the power pump is arranged outside the second section of the pipeline.

6. The enrichment and cleaning system according to claim 4, characterized in that: All stop valves and power pumps are set on a setting platform; guide marks for facilitating pipeline setting are set on the platform, and the guide marks are set between the stop valves, which include a main guide line and 6 branch guide lines corresponding to the pipelines.

7. The enrichment and cleaning system according to claim 6, characterized in that: At least one color guide structure is set on the main guide line; at least one color guide structure is set on the branch guide line, and a color matching structure is set on the main line corresponding to the main guide line and the branch line corresponding to the branch guide line; the color guide structure is a color identification block or a color identification sticker set on the main branch guide line and the branch guide line, and the color matching structure is a color identification ring set on the main line and the branch line, and the color identification ring is sleeved on the outside of the pipeline.

8. The enrichment and cleaning system according to claim 7, characterized in that: Two color guide structures are arranged on the main guide line, and two color guide structures are arranged on the branch guide line, wherein the two color guide structures on the main guide line are arranged on the first section of the pipeline and the third section of the pipeline; the two color guide structures on the branch guide line are arranged on the branch guide line corresponding to the output pipeline 2 and the input pipeline 2 connected to the centrifugal enrichment structure; wherein the two color guide structures on the main branch guide line have different colors, and the two color guide structures on the branch guide line have different colors; Alternatively, two color guide structures are set on the main guide line, and two color guide structures are set on the branch guide line, wherein the two color guide structures on the main guide line are set on the first section of the pipeline and the third section of the pipeline; the two color guide structures on the branch guide line are set on branch guide lines corresponding to an output pipeline one and an input pipeline one connected to the fat processing structure; wherein the two color guide structures on the main branch guide line have different colors; and the two color guide structures on the branch guide line have different colors.

9. The enrichment and cleaning system according to claim 2, characterized in that: The centrifugal enrichment structure is arranged in a centrifugal chamber with a cover, and the output pipeline 2 and the input pipeline 2 extend under the cover of the centrifugal chamber and onto the central axis pipeline of the centrifugal enrichment structure; the centrifugal enrichment device includes a transverse enrichment structure, and the central axis pipeline is arranged in the middle of the transverse enrichment structure; the rotation of the centrifugal enrichment structure will not drive the rotation of the central axis pipeline.

10. The enrichment and cleaning system according to claim 9, characterized in that: The central axis pipeline is rotatably connected to the transverse enrichment structure, and sealing materials are provided at the connection to maintain a leak-proof seal; Alternatively, a temperature maintenance structure is provided on the hatch cover, the temperature maintenance structure is a temperature maintenance ring extending from the center of the hatch cover and surrounding the central axis pipeline, a longitudinal notch is provided on the temperature maintenance ring for connecting the second output pipeline with the second input pipeline; the wire of the temperature maintenance structure is guided to the power supply position through the hatch cover; Alternatively, a heating plate is provided at the bottom of the centrifuge chamber to maintain the temperature of the entire centrifuge chamber.

11. A method for enriching and cleaning fat matrix components using the enrichment and cleaning system according to any one of claims 2 to 10, characterized in that: S1: The extracted fat is sent to the fat processing structure, and is left to stand for separation. After separation, the waste liquid is sent to the waste liquid collection container through the transmission path 2, and the fat is weighed until a sufficient amount of fat is reached; S2: washing of fat tissue, transferring the buffer from the buffer storage container to the fat processing structure through the transmission path 1 to wash the fat, and after washing, transferring the waste liquid to the waste liquid collection container through the transmission path 2; S3: lysis of adipose tissue, adding a lysis solvent to the fat processing structure, and then transferring the buffer from the buffer storage container to the fat processing structure through the transmission path 1 to lyse the washed fat, and the post-processing tank is left to stand for five minutes for separation to obtain the fat matrix component; S4: enrichment of fat matrix components. The separated fat matrix components are transferred to a centrifugal enrichment device through a transmission path three for enrichment. After enrichment, the waste liquid is transferred to a waste liquid collection container through a transmission path four; S5: Cleaning of fat matrix components. After the fat matrix components are enriched, the fat matrix components are cleaned in the centrifugal chamber. The buffer solution is transferred to the centrifugal enrichment structure for cleaning using the transmission path five. After cleaning, the waste liquid is discharged using the transmission paths four times.

12. A method for preparing a fat matrix component, characterized in that: Directly utilize the fat matrix component obtained by the method described in claim 11.

13. The method for preparing a fat matrix component according to claim 12, characterized in that: Mixing the fat matrix component obtained by the method of claim 11 with the washed fat; The steps for preparing the washed fat are as follows: injecting the fat aspirate into the washed fat processing structure for separation, discharging the waste liquid after separation and then washing, and discharging the waste liquid after washing.

14. The method for preparing a fat matrix component according to claim 13, characterized in that: The washing process of the lipoaspirate in the fat processing structure and the washing process of the fat matrix components in the centrifugal enrichment structure are carried out simultaneously.

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