Fibroin three-dimensional bracket for nervus centralis remediation

A three-dimensional scaffold and silk fiber technology, which is applied in the repair of spinal cord defects in the central nervous system. In the field of three-dimensional silk scaffolds, it can solve the problems of poor strength and incompatibility of cells, and achieve reduced immune response, good mechanical properties and water retention. performance, superior biocompatibility

Inactive Publication Date: 2017-05-17
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Prior to this, people used chitosan as a scaffold for repairing the spinal cord. Although the scaffold is easy to prepare, has controllable mechanical and degradation properties, and is conducive to the adhesion of related molecules and cell growth, it has the...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Degumming mulberry silk raw silk in 0.6‰ boiling aqueous solution of sodium carbonate with a mass fraction of 0.6‰ for 3 times, each treatment for 30 minutes for the first two times, and 45 minutes for the third treatment, the silk fiber obtained was washed with deionized water, and Dry in an oven at 55°C to obtain silk fibers.

[0027] (2) Place the silk fiber obtained in step (1) in a 9.3M lithium bromide solution, dissolve it at 60°C for 4 hours, put the resulting mixed solution into a dialysis bag (molecular weight 8000-14000) and dialyze it in deionized water for 4 days , during which the water was changed every two hours, and the concentration of the finally obtained silk fibroin solution was 5w / v%.

[0028] (3) The silk fiber prepared in the step (1) is treated with ion stripping technology to form a groove structure on its surface.

[0029] (4) Mix the silk fibroin solution prepared in step (2) with hyaluronic acid at a mass ratio of 50:50 to prepare a comp...

Embodiment 2

[0033] (1) Degumming mulberry silk raw silk in 0.6‰ boiling aqueous solution of sodium carbonate with a mass fraction of 0.6‰ for 3 times, each treatment for 30 minutes for the first two times, and 45 minutes for the third treatment, the silk fiber obtained was washed with deionized water, and Dry in an oven at 55°C to obtain silk fibers.

[0034] (2) Place the silk fiber obtained in step (1) in a 9.3M lithium bromide solution, dissolve it at 60°C for 4 hours, put the resulting mixed solution into a dialysis bag (molecular weight 8000-14000) and dialyze it in deionized water for 4 days , during which the water was changed every two hours, and the concentration of the finally obtained silk fibroin solution was 5w / v%.

[0035] (3) The silk fibroin fiber prepared in step (1) is prepared into a silk fibroin / polypyrrole composite conductive fiber by in situ adsorption polymerization, so that the polypyrrole is evenly distributed on the surface of the silk fibroin to form a layer of...

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PUM

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Abstract

The invention relates to a fibroin three-dimensional bracket for nervus centralis remediation, and belongs to the field of biological medicinal materials. The fibroin three-dimensional bracket comprises fibroin protein fiber with grooves, fibroin protein/hyaluronic acid plural gel and fibroin membrane conduits. By adopting the fibroin three-dimensional bracket, defects that a conventional bracket is low in mechanical property, low in biodegradability and the like are overcome. By adopting the fibroin three-dimensional bracket, physiological states of an extracellular matrix can be simulated, adhesion, migration, amplification and differentiation of cells can be facilitated, and furthermore orientated growth of axon to fracture parts can be guided; due to connection of the bracket, glial scars can be inhibited, and thus remediation of a nervus centralis system can be facilitated; and the bracket can be applied to basic research on neural injury remediation, can be also applied to bridging remediation of human body spinal cord injury or deficiency, and has good biomedical prospects.

Description

technical field [0001] The invention relates to a three-dimensional silk fibroin bracket which can be used for central nervous system repair, is mainly used for repairing spinal cord defects in the central nervous system, and belongs to the field of biomedical materials. Background technique [0002] Spinal cord injury is a serious injury to the central nervous system, and its repair is still one of the clinical problems. This is mainly because the self-repair ability is limited after spinal cord injury, and the damaged nerve cells are difficult to regenerate. At the same time, myelin-related inhibitory factors in the body will inhibit the regeneration of axons, which is closely related to functional recovery, and finally lead to the formation of scar barriers. [0003] With the continuous progress and wide application of new technology and technology, the research on the repair of spinal cord injury has made some progress, but it still cannot meet the requirements of clinic...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/26
CPCA61L27/50A61L27/26A61L2430/32
Inventor 张强张捷卢晨闫书芹尤仁传周应山顾绍金杨红军曹新旺肖仕丽
Owner WUHAN TEXTILE UNIV
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