33 results about "Cornea repair" patented technology

The invention discloses a preparation method of a cornea repair graft with biological activity and the cornea repair graft, and solves the technical problem of deficiency of cornea donors. The preparation method comprises the following steps: preparing collagen dispersion; preparing a collagen membrane on the basis of the collagen dispersion; adding surface functionalization modified substances on the surface of the collagen membrane in a 3D printing or coating mode under the sterile condition to form the cornea repair graft with biological activity. The surface functionalization modified substances are fine particles which are prepared from a cornea stroma, which does not contain a lesion part and contains active cells, of a patient, the biology and the biological stability of the high-strength collagen membrane can be improved, the physiological function of cornea is improved, after the modified collagen membrane is implanted into an eye of the patient, transparency and moisture content are good, the transparency can be recovered quickly clinically, the biological compatibility is good, and a good stent is provided for cornea cell culture of a cornea transplantation receptor.

The invention discloses a pharmaceutical composition for corneal tissue repair by an insulin nano system and application of the pharmaceutical composition, and belongs to the technical field of pharmaceutical compositions, and the pharmaceutical composition is characterized by comprising nano materials such as lipidosome and insulin wrapped in the nano materials such as lipidosome; the pharmaceutical composition for corneal tissue repair by the insulin nano system is applied to corneal repair. The invention is mainly used for providing a novel preparation ophthalmic pharmaceutical composition which has the advantages of safety, effectiveness, side effect and low administration frequency, and can effectively improve the compliance of patients; meanwhile, the ophthalmic pharmaceutical composition has important biological effects of resisting inflammation, resisting apoptosis and promoting corneal cell and nerve repair, and has more advantages in the treatment effects of resisting inflammation, resisting infection, promoting corneal repair and strengthening amniotic transplantation compared with a traditional treatment method.

A keratoprosthesis and system and method of using same for corneal repair. The keratoprosthesis comprises a biocompatible support and an optic member disposed through a channel within the support. The support includes metal, preferably titanium, and treated, such as by sandblasting and / or acid etching, to create textured surfaces that promote soft tissue adhesion. A locking member interconnects the optic member and support. An outer surface of the locking member a collar extending from the support and disposed around the optic member is also metal, preferably titanium, and is similarly treated to promote soft tissue adhesion. A locking member interconnects the optic member and support. The system includes the keratoprosthesis positioned within an isolated soft tissue segment of a non-ocular tissue, such as buccal mucosa, placed on the anterior cornea. The method includes removing corneal epithelium, isolating and transplanting a segment of soft tissue to the de-epithelialized cornea, creating a receiving area in the soft tissue, positioning a keratoprosthesis relative to the receiving area anterior to the cornea, and securing the keratoprosthesis.

The invention discloses an oxygen permeability test device and an oxygen permeability test method. The device comprises two sample clamps, an oxygen feeding device, a carrier gas feeding device and a detection device, wherein the sample clamps consist of oxygen cavities and carrier gas cavities; the oxygen cavities are connected with the oxygen feeding device through oxygen cavity feeding pipelines and are further provided with oxygen cavity discharging pipelines; the carrier gas cavities are connected with the carrier gas feeding device through carrier gas cavity feeding pipelines and are connected with the detection device through carrier gas cavity discharging pipelines; the detection device comprise a quantitative sample injection device and a gas chromatograph. The method disclosed by the invention comprises the following steps: placing a sample into the sample clamps, and feeding oxygen and carrier gas respectively to two sides of the sample. The oxygen enters the carrier gas through the sample, and the carrier gas is detected to obtain oxygen permeability data of the material. The device and the method can be used for testing the oxygen permeability of a cornea repair material; the device is long in service life, low in operation cost, small in system error and high in test precision.

The preparation method comprises the following steps: 1) preparing a silk fibroin solution, designing and optimizing 3D printing parameters of a silk fibroin annular stent, and constructing a plurality of silk fibroin annular stent materials with regular three-dimensional space structures; 2) carrying out surface chemical modification on the obtained silk fibroin annular scaffold material through a citric acid molecular solution; (3) preparing a collagen solution, introducing the carboxylated fibroin annular scaffold obtained in the step (2) into a collagen solution system, and enabling fibroin molecules and collagen molecules to form amido bond combination by taking EDC and NHS as a cross-linking agent and a catalyst respectively, so as to obtain a collagen solution; and finally, the silk fibroin reinforced collagen-based cornea repair material which is doubly compounded on a macroscopic physical structure and a microscopic chemical assembly level is formed. The material obtained by the invention has better physical and chemical properties and biocompatibility, is controllable in molding, and can be used for clinical repair and treatment of corneal tissue defects.

The invention discloses a surface functionalized chitosan cornea rehabilitating material and a preparation method thereof. The method comprises the following steps: dissolving chitosan into acid solution; injecting the chitosan solution into a mould to be dried; and drying the chitosan solution after being processed in alkali solution to obtain chitosan cornea. Biomacromolecule and short peptide are fixed on the surface of the chitosan cornea by adopting the chemical crosslinking method. The contact angle of the surface functionalized chitosan cornea declines obviously and the hydrophilic property is improved. The prepared surface functionalized chitosan cornea rehabilitating material has over 95 percent of light transmission, good cell compatibility and biologic compatibility, can be used for rehabilitating corneal damage in medical fields, and has the advantages of simple preparation process, simple equipment, cheap and accessible raw materials, good application prospect and scientific value.

A preparation method of an antibacterial cornea repair material includes (1) purifying type I collagen extracted from beef tendons, and preparing a collagen solution; (2) casting the collagen solution into a mold to form a membrane by air drying at room temperature; (3) soaking the membrane in an antibiotic solution, stirring until the membrane is in contact with the antibiotic solution, adding a crosslinking agent and a catalyst to the antibiotic solution contacted with the membrane, and stirring the antibiotic solution contacted with the membrane with the crosslinking agent and the catalyst added therein to perform a crosslinking reaction to obtain a crosslinked membrane; and (4) washing the crosslinked membrane material with deionized water, and air drying at room temperature. The antibacterial cornea repair material has relatively good mechanical properties, optical properties, biocompatibility, and antibacterial effect, and can be used for repairing and replacing damaged cornea tissue.

The invention relates to the technical field of medical biomaterials, in particular to a culture medium, a corneal stroma sheet prepared by it and a preparation method. The medium provided by the invention is a DMEM / F12 medium containing porcine corneal extract, Y-27632, ITS, FGF, ascorbic acid and fetal calf serum. Compared with the corneal stromal cells and corneal stromal sheets prepared by the traditional culture medium, the present invention has the ability to maintain keratocytes by selecting appropriate active components and proportioning ratios. Phenotype, promote proliferation, improve vitality, anti-fibrosis and anti-apoptosis and other functions. The corneal stroma sheet of the present invention is prepared by using corneal lens as a raw material and cultured in the medium. Compared with animal-derived corneal repair materials, it has good biocompatibility and no antigenicity, and can be used for various supplies of corneal transplantation. It is an alternative to body materials, which can be accepted by a large number of patients and used in clinical practice for a long time.

The invention discloses a preparation method of a cornea repair graft with biological activity and the cornea repair graft, and solves the technical problem of deficiency of cornea donors. The preparation method comprises the following steps: preparing collagen dispersion; preparing a collagen membrane on the basis of the collagen dispersion; adding surface functionalization modified substances on the surface of the collagen membrane in a 3D printing or coating mode under the sterile condition to form the cornea repair graft with biological activity. The surface functionalization modified substances are fine particles which are prepared from a cornea stroma, which does not contain a lesion part and contains active cells, of a patient, the biology and the biological stability of the high-strength collagen membrane can be improved, the physiological function of cornea is improved, after the modified collagen membrane is implanted into an eye of the patient, transparency and moisture content are good, the transparency can be recovered quickly clinically, the biological compatibility is good, and a good stent is provided for cornea cell culture of a cornea transplantation receptor.

The invention discloses a cornea repairing material with matrix repairing capability and a preparation method thereof. The method comprises the following steps: (1) preparing a microRNA solution; (2) preparing a collagen solution; (3) preparing a collagen membrane; (4) preparing a gold nano-particle solution; (5) preparing a microRNA and gold nano-particle mixed solution; and (6) dropwise adding the mixed solution onto the collagen membrane to obtain the cornea repairing material with matrix repairing capability. The cornea repairing material has a laminar structure, has the advantages of good transparency, high moisture content and excellent ion permeation performance, and can be used for effectively reducing and retarding occurrence of cicatrix repairing. The cornea repairing material with matrix repairing capability can be used for repairing a damaged cornea matrix in the medical field, is prepared from low-price and easily available raw materials, is simple in preparation process and simple in equipment, and has excellent application prospects and scientific values.

The present invention relates to the application of a short-chain peptide composition in preventing or treating dry eye. The short-chain peptide composition of the present invention comprises a short-chain peptide composed of 5-10 amino acids, and has the effects of promoting corneal repair, increasing eye humidity and improving dry eye syndrome.

The invention discloses a preparation method for an antifungal cornea repairing material. The preparation method comprises the following steps: (1) preparing a saturated solution of dialdehyde beta-cyclodextrin, adding a medicine and carrying out a full reaction so as to obtain a dialdehyde beta-cyclodextrin / medicine inclusion complex; (2) extracting I type collagen, subjecting the extracted I type collagen to freeze-drying and dissolving the freeze-dried I type collagen in an acetic acid or hydrochloric acid solution to prepare a collagen solution; (3) adding the inclusion complex obtained inthe step (1) as a cross-linking agent into the collagen solution to remove bubbles in the solution; and (4) casting the cross-linked collagen solution to a cornea-repairing-material forming die for film formation. The material prepared by using the method has high transparency, good mechanical properties, and temperature-sensitive release properties, is obviously different in releasing effect atdifferent temperatures, and can be used for different medical fields.

The invention discloses a GelMA-collagen dual-network antibacterial cornea repair material as well as a preparation method and application thereof. The preparation method of the cornea repair material comprises the following steps: S1, uniformly mixing a lysozyme solution and a methacrylated gelatin solution, and adding a cross-linking agent for cross-linking to obtain a cross-linked product; S2, blending the cross-linked product with a collagen solution, adding a cross-linking agent for cross-linking, then adding a photoinitiator, and conducting reacting in a dark place at room temperature to obtain a uniform and bubble-free mixed solution; and S3, performing ultraviolet crosslinking on the mixed solution in S2, and performing air drying to form a film, thereby obtaining the GelMA-collagen dual-network antibacterial cornea repair material. The GelMA-collagen dual-network antibacterial cornea repair material has good biocompatibility and biodegradability, has a layered structure, is good in optical permeability and water-containing performance and has certain mechanical properties, tearing is not caused when an operation line is used for suturing, and the cornea repair material also has an inhibition effect on gram-negative bacteria and gram-positive bacteria, and has excellent antibacterial performance.

The invention discloses bio-ink for 3D printing of a cornea repair material. The bio-ink comprises methacrylamide gelatin, a photoinitiator, collagen and a solvent. The concentration of the methacrylamide gelatin in the bio-ink is 50-150 mg / mL; the concentration of the photoinitiator in the bio-ink is 1-5 mg / mL; and the concentration of the collagen solution in the bio-ink is 0.5-6.0 mg / mL. The invention further discloses a preparation method of the bio-ink for 3D printing of the cornea repair material and a preparation method of the cornea repair material based on the bio-ink for 3D printing of the cornea repair material. The printing precision of the cornea repair material is improved, the mechanical property of the printing stent is improved, and the printing stent has a complete curvature structure, is good in optical permeability and water-containing performance and can fully meet the requirements of growth and proliferation of cornea epithelial cells.

An ophthalmic product having a cornea repair function includes an ophthalmic composition. The ophthalmic composition includes gold nanoparticles serving as the main repairing ingredient and at least one auxiliary repairing ingredient. The effective concentration of the gold nanoparticles is from 0.01 ppm to 3000 ppm. The content of the at least one auxiliary repairing ingredient is greater than 0 wt % and less than 20 wt % based on 100 wt % of the ophthalmic composition.

The invention belongs to the field of biological medicine, and discloses cornea repair hydrogel and a preparation method and application thereof. The cornea repair hydrogel is prepared through a cross-linking reaction of an IKK-beta specific small-molecule inhibitor and silk fibroin, and the preparation method of the cornea repair hydrogel specifically comprises the following steps of 1, using mulberry silk as a raw material, performing degumming, dissolving, dialysis and centrifugation, and obtaining a silk fibroin solution; (2) adding a TPCA-1 solution into the silk fibroin solution obtained in the step (1), and uniformly mixing; and (3) carrying out ultrasonic treatment on the mixed solution of the silk fibroin and the TPCA-1 obtained in the step (2), and carrying out cross-linking reaction at 37 DEG C to obtain the silk fibroin hydrogel compounded with the TPCA-1. The cornea repair hydrogel prepared by the invention is hydrophilic gel with a three-dimensional network structure, has certain adhesion to a cornea injury surface, is suitable for cornea injury repair, is simple in preparation process, does not use additives such as a denaturing agent, a coagulant and a cross-linking agent in the preparation process, and ensures the biological activity of TPCA-1 to the greatest extent.

The present invention relates to an ophthalmic product with cornea repair function. The ophthalmic product having a cornea repair function includes an ophthalmic composition. The ophthalmic composition includes gold nanoparticles serving as the main repairing ingredient and at least one auxiliary repairing ingredient. An effective concentration of the gold nanoparticles is from 0.01 ppm to 3000 ppm. The content of the at least one auxiliary repairing ingredient is greater than 0 wt % and less than 20 wt % based on 100 wt % of the ophthalmic composition. The ophthalmic product can enhance the protection and repairing capacity of cells in each layer of tissues of the eyes, and can improve the wearing comfort of the contact lenses.

The invention discloses a preparation method of a cornea repair material of a double-layer structure, and relates to the related fields of biomedical materials, medical apparatuses and instruments, cosmetics and the like. The method includes the following steps of dissolving collagen in weak acid to obtain a collagen solution of a certain concentration, preparing polyvinyl alcohol (PVA) into a solution, pouring the solution into a mold, conducting drying forming to obtain a polyvinyl alcohol film, laying the collagen solution on the surface of the polyvinyl alcohol membrane flat, and conducting drying to obtain a collagen compound membrane. The obtained compound membrane solves the problem that an existing collagen material is not enough in mechanical strength, and can promote adherency and proliferation of cells, and a new way is provided for application of collagen. The preparation method is simple in preparation process, high in repeatability and suitable for industrialized large-scale production of modified collagen compound films with different requirements.