Method for preparing surface-functionalized chitosan cornea repair material

A technology of chitosan and repair materials, which is applied in the field of preparation of surface-functionalized chitosan corneal repair materials, can solve problems such as inability to exchange nutrients and metabolites in time, difficulty in cell adhesion, proliferation, and poor biocompatibility. Achieve the effects of improving biological activity and biocompatibility, good growth and proliferation, and low cost

Active Publication Date: 2012-12-19
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional artificial cornea materials have poor biocompatibility, it is difficult for cells to truly adhere and proliferate, nutrients and metabolites cannot be exchanged in time, and there are complex interface problems between the stent and the light column, and the stent and eye tissue, and the incidence of inflammation is high

Method used

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  • Method for preparing surface-functionalized chitosan cornea repair material
  • Method for preparing surface-functionalized chitosan cornea repair material
  • Method for preparing surface-functionalized chitosan cornea repair material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation method of surface functionalized chitosan corneal repair material comprises the following steps:

[0042] (1) 0.4g chitosan is dissolved in the acetic acid aqueous solution of 20ml 1%wt;

[0043] (2) remove air bubbles with the chitosan acetic acid aqueous solution of step (1), air-dry film-forming under the room temperature of injecting mould;

[0044] (3) Add 2%wt sodium hydroxide solution to the mold of step (2), so that the film is fully immersed in the solution, remove the film after 30 minutes, rinse and soak in deionized water for 2 hours;

[0045] (4) Soak the film obtained in step (3) completely in 1%wt gelatin solution, and add 0.5%wt crosslinking agent for crosslinking treatment, so that the mass ratio of gelatin to EDC=6:1. Stir to disperse the cross-linking agent evenly, the reaction temperature is 35°C, and the reaction time is 24 hours;

[0046] (5) Rinse the product of step (4) with deionized water, and air dry naturally at room tempera...

Embodiment 2

[0056] The preparation method of surface functionalized chitosan corneal repair material comprises the following steps:

[0057] (1) 0.4g chitosan is dissolved in the acetic acid aqueous solution of 20ml 3%wt;

[0058] (2) remove air bubbles with the chitosan acetic acid aqueous solution of step (1), air-dry film-forming under the room temperature of injecting mould;

[0059] (3) Add 5%wt ammonia solution to the mold of step (2), so that the film is completely immersed in the solution, remove the film after 30 minutes, rinse and soak in deionized water for 3 hours;

[0060] (4) Soak the film obtained in step (3) completely in 5%wt gelatin solution, and add 0.5%wt crosslinking agent for crosslinking treatment, so that the mass ratio of gelatin to EDC=6:1. Stir to disperse the cross-linking agent evenly, the reaction temperature is 25°C, and the reaction time is 24 hours;

[0061] (5) Rinse the product of step (4) with deionized water, and air dry naturally at room temperature...

Embodiment 3

[0064] The preparation method of surface functionalized chitosan corneal repair material comprises the following steps:

[0065] (1) 0.4g chitosan is dissolved in the acetic acid aqueous solution of 20ml 2%wt;

[0066] (2) remove air bubbles with the chitosan acetic acid aqueous solution of step (1), air-dry film-forming under the room temperature of injecting mould;

[0067] (3) Add 5%wt sodium hydroxide solution to the mold of step (2), so that the film is fully immersed in the solution, remove the film after 30 minutes, rinse and soak in deionized water for 4 hours;

[0068] (4) Soak the film obtained in step (3) completely in 0.5%wt gelatin solution, and add 0.5%wt crosslinking agent for crosslinking treatment, so that the mass ratio of gelatin to EDC=6:1. Stir to disperse the crosslinking agent evenly, the reaction temperature is 25°C, and the reaction time is 48 hours;

[0069] (5) Rinse the product of step (4) with deionized water, and air dry naturally at room temper...

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Abstract

The invention discloses a surface functionalized chitosan cornea rehabilitating material and a preparation method thereof. The method comprises the following steps: dissolving chitosan into acid solution; injecting the chitosan solution into a mould to be dried; and drying the chitosan solution after being processed in alkali solution to obtain chitosan cornea. Biomacromolecule and short peptide are fixed on the surface of the chitosan cornea by adopting the chemical crosslinking method. The contact angle of the surface functionalized chitosan cornea declines obviously and the hydrophilic property is improved. The prepared surface functionalized chitosan cornea rehabilitating material has over 95 percent of light transmission, good cell compatibility and biologic compatibility, can be used for rehabilitating corneal damage in medical fields, and has the advantages of simple preparation process, simple equipment, cheap and accessible raw materials, good application prospect and scientific value.

Description

technical field [0001] The invention relates to a preparation method of a corneal repair material, in particular to a preparation method of a surface-functionalized chitosan corneal repair material. Background technique [0002] Corneal disease is one of the most important reversible and blinding eye diseases in the world today. According to the statistics of the World Health Organization: corneal disease is the second most common blindness disease in the world after cataract. Nearly 1 / 3 of the blind patients in the world are caused by corneal disease. my country is a country with a high incidence of corneal diseases. According to incomplete statistics, there are more than 4 million blind people in my country due to corneal diseases, accounting for the second place among all kinds of blinding eye diseases. However, most corneal blindness can be treated by surgery, and corneal transplantation is one of the important treatments for corneal blindness. However, the main proble...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/22A61L27/20
Inventor 王迎军任力郭丽
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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