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Surface immobilized modified cornea repair material and preparation method thereof

A technology for repairing materials and corneas, which is applied in medical science, prostheses, tissue regeneration, etc. It can solve the problems of poor mechanical properties, light transmission, oxygen permeability, and film-forming properties, and cannot meet the clinical needs of corneal repair materials. Good physical and chemical properties, large-scale mass production, and good repeatability

Inactive Publication Date: 2017-09-22
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most corneal repair materials prepared from natural polymers have poor mechanical properties, light transmission, oxygen permeability and film-forming properties, which cannot meet the clinical needs of corneal repair materials; they usually need to be compounded, cross-linked, and modified. and other means to improve the indicators of collagen repair materials to realize the possibility of its application

Method used

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  • Surface immobilized modified cornea repair material and preparation method thereof
  • Surface immobilized modified cornea repair material and preparation method thereof
  • Surface immobilized modified cornea repair material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Dissolve gelatin in 0% acetic acid solution, stir mechanically at 60°C for 1 hour (stirring speed is 700r / min), prepare a gelatin solution with a weight ratio of 20wt%, then inject the gelatin solution into a mold, and dry it naturally at room temperature Get gelatin film for later use. Get chitosan and cross-linking agent EDC / NHS (mass ratio cross-linking agent / chitosan=1 / 700) and dissolve in 5% hydrochloric acid solution, under the condition of 25 ℃ mechanical stirring 24h (stirring speed is 100r / min) , preparing a chitosan mixed solution with a weight ratio of 3wt%, soaking the prepared gelatin film in the chitosan mixed solution for 2 hours, taking it out at room temperature and drying it naturally to obtain a surface-immobilized modified gelatin corneal repair material. After immobilization and modification, the roughness of the membrane surface is significantly improved, which is more conducive to cell adhesion and growth. figure 1 Shown are the microscopic topog...

Embodiment 2

[0026] Get chitosan and be dissolved in 0.5% hydrochloric acid solution, under the condition of 50 ℃ of mechanical stirring 8h (stirring speed is 500r / min), preparation weight ratio is the chitosan solution of 5wt%, then chitosan solution is injected in the mould. , dried naturally at room temperature to prepare chitosan film for later use. Sodium hyaluronate and cross-linking agent glutaraldehyde (mass ratio cross-linking agent / sodium hyaluronate=1 / 100) were dissolved in 1% acetic acid solution, and mechanically stirred for 36 hours at 10°C (stirring rate was 100r / min), prepare a sodium hyaluronate mixed solution with a weight ratio of 15wt%; soak the prepared chitosan film in the sodium hyaluronate mixed solution for 1 hour, take it out and dry it under vacuum at 30°C to obtain the surface-immobilized modified shell Polysaccharide corneal repair material. A single polymer film is immobilized and modified, which can effectively improve the transparency of corneal repair mat...

Embodiment 3

[0028] Dissolve collagen in 5% hydrochloric acid solution, stir mechanically at 10°C for 36h (stirring rate is 100r / min), prepare a collagen solution with a weight ratio of 1wt%, then inject the collagen solution into a mold, and dry it naturally at room temperature. Get the collagen film for later use. Sodium hyaluronate and cross-linking agent EDC / NHS (mass ratio cross-linking agent / sodium hyaluronate=1 / 1000) were dissolved in 1.5% hydrochloric acid solution, and mechanically stirred for 18 hours at 30°C (stirring speed was 200r / min), prepare a sodium hyaluronate mixed solution with a weight ratio of 1 wt%; soak the prepared gelatin film in the sodium hyaluronate mixed solution for 36 hours, take it out and dry it under vacuum at 50°C to obtain the surface-immobilized modified collagen corneal repair Material. After the gelatin film is immobilized and modified, the contact angle decreases, indicating that its surface hydrophilicity is enhanced, which is more conducive to k...

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Abstract

The invention discloses a method for preparing a surface immobilized modified cornea repair material. The method comprises the following specific steps: (1) adding a high polymer material a into a solvent for mechanically stirring, preparing a natural high polymer solution I with the weight ratio of 0.1-15wt%, and injecting the high polymer solution I into a mold so as to prepare a high polymer film; (2) preparing a high polymer solution II from a high polymer material b and a cross-linking agent according to the method in the step (1), wherein a and b are different high polymer materials; (3) drying the high polymer film in the step (1), soaking the film in the solution II prepared in the step (2), soaking for 0.5-36 hours, taking out and drying the film, thereby obtaining the surface immobilized modified cornea repair material. The preparation method is simple in process, high in repeatability and capable of realizing large-scale volume production, and the prepared material has excellent physical and chemical properties and is excellent in repair effect.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a surface-immobilized modified corneal repair material and a preparation method thereof. Background technique [0002] The cornea is the first window through which external light enters the eye, and together with the sclera constitutes the natural defense line of the eyeball. The transparent texture of the cornea is an important part of the refractive interstitium; its biggest feature is that it does not contain blood vessels inside, and the nutrients it needs are mainly supplied by the corneal limbal vascular network, which is in a relatively "pardoned" status in immunology. The rate is the highest of all organ transplants. The normal cornea can be divided into five layers from superficial to deep: corneal epithelium, Bowman's membrane, stroma, Bowman's membrane and endothelial layer. Among them, the epithelial layer is in direct contact with the outside world, so the probabi...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/24A61L27/20
CPCA61L27/20A61L27/222A61L27/227A61L27/24A61L2430/16C08L89/00C08L5/08
Inventor 任力
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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