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Preparation method of cornea repair graft with biological activity and cornea repair graft

A bioactive, corneal technology, applied in the field of bioactive corneal repair grafts, can solve problems such as the lack of corneal donors, achieve the effects of improving physiological functions, solving the lack of corneal donors, and good biocompatibility

Active Publication Date: 2016-10-26
广州尤尼智康生物科技有限公司
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Problems solved by technology

[0005] This application provides a preparation method of a bioactive corneal repair graft and a corneal repair graft to solve the technical problem of lack of corneal donors

Method used

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  • Preparation method of cornea repair graft with biological activity and cornea repair graft

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preparation example Construction

[0022] The preparation method of the bioactive corneal repair graft proposed by the present invention, such as figure 1 shown, including the following steps:

[0023] Step S11: Prepare collagen swelling solution.

[0024] Specifically, collagen is extracted from the hoof tissue of animals by protease digestion; then the extracted collagen is swelled with 0.3%-0.5% malonic acid solution, mixed and centrifuged to remove impurities, and collagen swelling solution is obtained. Store at 4°C for later use.

[0025] Step S12: Prepare a collagen film based on the collagen swelling solution.

[0026] Specifically, the collagen swelling solution is salted out and concentrated with a saturated inorganic salt solution to obtain a collagen suspension solution; the collagen suspension solution is centrifuged and separated and precipitated to obtain a collagen gel with a water content of less than 10%; the collagen gel is placed in a mold , freeze-dried after low-temperature freezing; put...

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Abstract

The invention discloses a preparation method of a cornea repair graft with biological activity and the cornea repair graft, and solves the technical problem of deficiency of cornea donors. The preparation method comprises the following steps: preparing collagen dispersion; preparing a collagen membrane on the basis of the collagen dispersion; adding surface functionalization modified substances on the surface of the collagen membrane in a 3D printing or coating mode under the sterile condition to form the cornea repair graft with biological activity. The surface functionalization modified substances are fine particles which are prepared from a cornea stroma, which does not contain a lesion part and contains active cells, of a patient, the biology and the biological stability of the high-strength collagen membrane can be improved, the physiological function of cornea is improved, after the modified collagen membrane is implanted into an eye of the patient, transparency and moisture content are good, the transparency can be recovered quickly clinically, the biological compatibility is good, and a good stent is provided for cornea cell culture of a cornea transplantation receptor.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a preparation method of a bioactive corneal repair graft and the bioactive corneal repair graft prepared by the preparation method. Background technique [0002] The cornea is a transparent tissue that is connected to the anterior wall of the eyeball and mainly performs barrier and refractive functions. [0003] Because the cornea is in direct contact with the outside world, it is extremely vulnerable to mechanical trauma, thermal burns, acid and alkali erosion, etc., which will cause corneal diseases, and even blindness in severe cases. Corneal blindness is now the second leading cause of blindness in the world. Tens of millions of people around the world lose their sight due to corneal diseases every year, and 80% of them can be relieved of blindness through corneal transplantation. [0004] However, corneal blindness cannot be relieved through corneal t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/36A61L27/50
CPCA61L27/24A61L27/3604A61L27/50A61L2400/18A61L2430/16C08L89/00
Inventor 王红张楠陈静
Owner 广州尤尼智康生物科技有限公司
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