Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of corneal repair material with stroma repair ability and preparation method thereof

A technology of repairing materials and capabilities, applied in prostheses, tissue regeneration, medical science, etc., can solve problems such as corneal scar repair, decreased cell transparency, and impact on vision, and achieve slow scar repair, good application prospects and scientific value , Ease of penetration and transportation

Active Publication Date: 2019-02-19
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the cornea is damaged, both the epithelial cell layer and the stromal layer are often damaged, and the damaged corneal epithelial cells will release TGF-β2 into the stromal layer, stimulating the transformation of corneal stromal cells into myofibroblasts, and myofibroblasts will secrete disorders The collagen fibers in the cornea are different from the arrangement of collagen fibers in the healthy corneal stroma tissue, which will cause the decrease of cell transparency, and eventually form scar repair of the cornea, which will affect vision

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of corneal repair material with stroma repair ability and preparation method thereof
  • A kind of corneal repair material with stroma repair ability and preparation method thereof
  • A kind of corneal repair material with stroma repair ability and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0039] A preparation method of a corneal repair material with matrix repair ability, comprising the following steps:

[0040] 1) The microRNA is prepared into a microRNA solution with DEPC-treated water;

[0041] 2) adding the collagen to the hydrochloric acid DEPC-treated aqueous solution or the acetic acid DEPC-treated aqueous solution, stirring evenly, and removing the bubbles in the solution to obtain a collagen solution;

[0042] 3) Pour the collagen solution into a mold, dry to form a film, remove the mold, wash with water for many times, and dry to obtain a collagen film;

[0043] 4) Gold nanoparticles are prepared into a gold nanoparticle solution with DEPC-treated water;

[0044] 5) uniformly mixing the configured microRNA solution with the gold nanoparticle solution to obtain a mixed solution;

[0045] 6) Evenly drop the mixed solution in step 5) onto the collagen film in step 3), and dry to obtain a corneal repair material with matrix repair ability.

[0046] Pre...

Embodiment 1

[0066] 1) Prepare the microRNA-133b powder with DEPC-free water to prepare a microRNA solution with a concentration of 20 μmol / L;

[0067] 2) Treat type Ι collagen extracted from beef tendon with 0.001mol / L hydrochloric acid DEPC to prepare a collagen solution with a concentration of 6.5mg / mL, then stir in a refrigerator at 4°C for 4 hours, and then stir at room temperature for 24 hours , to remove air bubbles;

[0068] 3) Pour the solution in step 2) into the mold, air-dry naturally at room temperature to form a film, wash with deionized water several times, and air-dry naturally at room temperature to obtain a collagen film;

[0069] 4) Prepare the gold nanoparticles with DEPC-free water to prepare a gold nanoparticle solution with a concentration of 0.03 mg / mL;

[0070] 5) Mix the microRNA solution and the gold nanoparticle solution evenly according to the mass ratio of microRNA and gold nanoparticles of 1:3 to obtain a mixed solution;

[0071] 6) Dilute the mixed solutio...

Embodiment 2

[0073] 1) Prepare the microRNA-133b powder with DEPC-free water to prepare a microRNA solution with a concentration of 25 μmol / L;

[0074] 2) Treat type Ι collagen extracted from beef tendon with 0.1 mol / L acetic acid DEPC to prepare a collagen solution with a concentration of 6.0 mg / mL, then stir in a 4°C refrigerator for 5 hours, and then stir at room temperature for 20 hours, remove the air bubbles;

[0075] 3) Pour the solution in step 2) into the mold, air-dry naturally at room temperature to form a film, wash with deionized water several times, and air-dry naturally at room temperature to obtain a collagen film;

[0076] 4) The gold nanoparticles were prepared into a gold nanoparticle solution with a concentration of 0.025 mg / mL with DEPC-free treated water;

[0077] 5) Mix the microRNA solution and the gold nanoparticle solution evenly according to the mass ratio of microRNA and gold nanoparticles of 1:2 to obtain a mixed solution;

[0078] 6) The mixed solution in st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a cornea repairing material with matrix repairing capability and a preparation method thereof. The method comprises the following steps: (1) preparing a microRNA solution; (2) preparing a collagen solution; (3) preparing a collagen membrane; (4) preparing a gold nano-particle solution; (5) preparing a microRNA and gold nano-particle mixed solution; and (6) dropwise adding the mixed solution onto the collagen membrane to obtain the cornea repairing material with matrix repairing capability. The cornea repairing material has a laminar structure, has the advantages of good transparency, high moisture content and excellent ion permeation performance, and can be used for effectively reducing and retarding occurrence of cicatrix repairing. The cornea repairing material with matrix repairing capability can be used for repairing a damaged cornea matrix in the medical field, is prepared from low-price and easily available raw materials, is simple in preparation process and simple in equipment, and has excellent application prospects and scientific values.

Description

technical field [0001] The invention relates to a corneal repair material with matrix repair ability and a preparation method thereof. The corneal matrix repair material of the invention can be applied to the repair of damaged corneal matrix, and belongs to the field of biomedical materials. Background technique [0002] Corneal disease is a very common ophthalmic disease and the second most common cause of blindness in the world after cataracts. According to reports, about 10 million people in the world lose their sight due to corneal diseases every year. Now the only widely accepted treatment method is to use human corneal donors for transplantation. However, especially in developing countries, there is a shortage of donor corneas. In my country, no more than 5,000 corneal transplants can be performed each year. Therefore, the research and development of artificial corneal repair materials seems very necessary. [0003] Common corneal diseases include mechanical damage, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/24A61L27/04
CPCA61L27/047A61L27/24A61L27/54A61L2300/258A61L2430/16C08L89/00
Inventor 任力赵轩王迎军宋文婧陈娅伟
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com