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GelMA-collagen dual-network antibacterial cornea repair material as well as preparation method and application thereof

A repair material and dual-network technology, applied in medical science, prosthesis, tissue regeneration, etc., can solve problems such as inability to effectively inhibit bacterial infection, pathogen drug resistance, and affect the safety of corneal repair materials, and achieve the goal of reducing microbial infection Risk, excellent antibacterial properties, effective in solving postoperative infection

Active Publication Date: 2021-10-01
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned antibacterial corneal repair materials mainly improve the antibacterial properties of corneal repair materials by infiltrating cross-linked antibiotics, but frequent or other improper use of antibiotics will lead to drug resistance of pathogens, which may eventually fail to effectively inhibit bacterial infection and directly affect corneal repair materials security

Method used

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  • GelMA-collagen dual-network antibacterial cornea repair material as well as preparation method and application thereof
  • GelMA-collagen dual-network antibacterial cornea repair material as well as preparation method and application thereof
  • GelMA-collagen dual-network antibacterial cornea repair material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A GelMA-collagen double network antibacterial corneal repair material (GelMA-Col-LZM composite film, CGL), the preparation method comprises the following steps:

[0060] S1. According to the mass ratio of lysozyme and methacrylated gelatin is 1:10 to prepare the blend solution of the two, add EDC-NHS solution (the mass ratio of EDC and NHS is 1:1) for cross-linking, lysozyme - The mass ratio of methacrylated gelatin to EDC in the methacrylated gelatin blend solution is 6:1, and the solution after adding the crosslinking agent is stirred and reacted at 25° C. for 3 hours to obtain a crosslinked product;

[0061] S2. According to the mass ratio of methacrylylated gelatin and collagen is 1:2, the cross-linked product in S1 and collagen solution are formulated into a blend solution, and EDC-NHS solution is added (the mass ratio of EDC and NHS is 1:1 ) for cross-linking, the mass ratio of collagen and EDC in the blended solution is 6:1, and the solution after adding the cros...

Embodiment 2

[0075] A GelMA-collagen double network antibacterial corneal repair material, the preparation method comprising the following steps:

[0076] S1. According to the mass ratio of lysozyme and methacrylated gelatin is 1:8 to prepare the blend solution of the two, add EDC-NHS solution (the mass ratio of EDC, NHS is 1.5:1) for cross-linking, lysozyme - The mass ratio of methacrylated gelatin to EDC in the methacrylated gelatin blend solution is 4:1, and the solution after adding the crosslinking agent is stirred and reacted at 20° C. for 4 hours to obtain a crosslinked product;

[0077] S2. According to the mass ratio of methacrylated gelatin and collagen is 1:4, the cross-linked product in S1 and collagen solution are formulated into a blend solution, and EDC-NHS solution is added (the mass ratio of EDC and NHS is 1.5:1 ) for cross-linking, the mass ratio of collagen and EDC in the blended solution is 4:1, and the solution after adding the cross-linking agent is stirred and reacte...

Embodiment 3

[0084] A GelMA-collagen double network antibacterial corneal repair material, the preparation method comprising the following steps:

[0085] S1. According to the mass ratio of lysozyme and methacrylated gelatin is 1:6 to prepare the blend solution of the two, add EDC-NHS solution (the mass ratio of EDC and NHS is 2:1) to carry out cross-linking, lysozyme - The mass ratio of methacrylated gelatin to EDC in the methacrylated gelatin blend solution is 3:1, and the solution after adding the crosslinking agent is stirred and reacted at 22° C. for 2 hours to obtain a crosslinked product;

[0086] S2. According to the mass ratio of methacrylated gelatin and collagen is 1:6, the cross-linked product in S1 and collagen solution are formulated into a blend solution, and EDC-NHS solution is added (the mass ratio of EDC and NHS is 2:1 ) for cross-linking, the mass ratio of collagen and EDC in the blended solution is 3:1, and the solution after adding the cross-linking agent is stirred an...

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Abstract

The invention discloses a GelMA-collagen dual-network antibacterial cornea repair material as well as a preparation method and application thereof. The preparation method of the cornea repair material comprises the following steps: S1, uniformly mixing a lysozyme solution and a methacrylated gelatin solution, and adding a cross-linking agent for cross-linking to obtain a cross-linked product; S2, blending the cross-linked product with a collagen solution, adding a cross-linking agent for cross-linking, then adding a photoinitiator, and conducting reacting in a dark place at room temperature to obtain a uniform and bubble-free mixed solution; and S3, performing ultraviolet crosslinking on the mixed solution in S2, and performing air drying to form a film, thereby obtaining the GelMA-collagen dual-network antibacterial cornea repair material. The GelMA-collagen dual-network antibacterial cornea repair material has good biocompatibility and biodegradability, has a layered structure, is good in optical permeability and water-containing performance and has certain mechanical properties, tearing is not caused when an operation line is used for suturing, and the cornea repair material also has an inhibition effect on gram-negative bacteria and gram-positive bacteria, and has excellent antibacterial performance.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, more specifically, to a GelMA-collagen double network antibacterial corneal repair material and its preparation method and application. Background technique [0002] Corneal disease is a very common ophthalmic disease and one of the main causes of blindness. At present, the most effective way to treat corneal blindness is corneal transplantation. According to reports, about 15 million patients worldwide need corneal transplantation. However, there is a severe shortage of donor corneas in the world. Therefore, the research and development of corneal repair materials to replace donor corneas is very necessary. [0003] Appropriate corneal repair materials should have the water content, light transmittance, oxygen permeability and ion transmittance that match those of healthy human corneas, so as to meet the needs of corneal transparency and eye nutrient exchange. Corneas used for corne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/24A61L27/22A61L27/54A61L27/58
CPCA61L27/24A61L27/227A61L27/54A61L27/58A61L2430/16A61L2300/254A61L2300/404
Inventor 任力杨向璟孙晓敏
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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