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Bio-ink for 3D printing of cornea repair material, preparation method of bio-ink and preparation method of cornea repair material

A bio-ink and repair material technology, applied in tissue regeneration, additive processing, prosthesis, etc., can solve problems such as difficult printing, and achieve the effect of short time-consuming, high-throughput printing, and improved biological performance

Active Publication Date: 2021-12-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the above-mentioned shortcomings and deficiencies of the prior art, the object of the present invention is to provide a bio-ink for 3D printing corneal repair materials, improve the defects of pure GelMA low concentration and difficult to print, improve the printing accuracy of pure GelMA, and at the same time Due to the addition of bioactive collagen components, the mechanical and biological properties of the printed scaffold are improved

Method used

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  • Bio-ink for 3D printing of cornea repair material, preparation method of bio-ink and preparation method of cornea repair material
  • Bio-ink for 3D printing of cornea repair material, preparation method of bio-ink and preparation method of cornea repair material
  • Bio-ink for 3D printing of cornea repair material, preparation method of bio-ink and preparation method of cornea repair material

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Effect test

Embodiment 1

[0059] The bio-ink based on the 3D printing corneal repair material of the present embodiment includes methacrylamide-based gelatin, photoinitiator, collagen and solvent; in the present embodiment, the mass concentration of the methacrylamide-based gelatin in the bio-ink 100mg / mL; the mass concentration of the photoinitiator in the bioink is 5mg / mL; the mass concentration of the collagen in the bioink is 6.0mg / mL.

[0060] In the present embodiment, the grafting ratio of methacrylamide-based gelatin is 99%; the collagen is type I collagen extracted from bovine Achilles tendon; the photoinitiator is I2959 (2-hydroxyl-2-methyl-1-[ 4-(2-hydroxyethoxy)phenyl]-1-propanone); solvents include PBS and hydrochloric acid solution.

[0061] The preparation method of the bio-ink based on the 3D printing corneal repair material of the present embodiment is:

[0062] (1) GelMA freeze-dried solid with a grafting rate of 99% was dissolved in 1XPBS at 50° C. to prepare a GelMA solution with a...

Embodiment 2

[0069] The bio-ink based on the 3D printing corneal repair material of the present embodiment includes methacrylamide-based gelatin, photoinitiator, collagen and solvent; in the present embodiment, the mass concentration of the photoinitiator in the bio-ink is 5mg / mL; the mass concentration of the methacrylamide-based gelatin in the bio-ink is A mg / mL; the concentration of the collagen in the bio-ink is B mg / mL; the specific formula is shown in Table 1.

[0070] In this embodiment, the grafting rate of methacrylamide-based gelatin is 99%; the collagen is type I collagen extracted from bovine Achilles tendon; the photoinitiator is I2959; the solvent includes PBS and hydrochloric acid solution.

[0071] The preparation method of the bio-ink based on the 3D printing corneal repair material of the present embodiment is:

[0072] The GelMA freeze-dried solid with a grafting rate of 99% was dissolved in 1XPBS at 50° C. to prepare a GelMA solution with a mass concentration of Amg / mL...

Embodiment 3

[0081] Utilize the bio-ink prepared in embodiment 1 to realize the preparation method of corneal repair material, the steps are as follows:

[0082] (1) The bio-ink is placed in a low-temperature light-proof barrel for 3D printing. Set the temperature of the printer barrel to 30°C, the temperature of the printing platform to 10°C, the printing pressure to 0.035MPa, and the printing speed to 6mm / s. The ink is extruded onto the temperature-controlled cornea printing platform;

[0083] (2) Set the UV light intensity to 100mW / cm 2 , the printing speed is 8mm / s.

[0084] (3) Remove the corneal stent from the printing platform with tweezers to obtain the 3D printed corneal repair material of the present invention.

[0085] Specifically, this embodiment adopts such as Figure 5 The temperature-controlled cornea printing platform with the structure shown: the temperature-controlled cornea printing platform is provided with a plurality of bosses with circular cross-sections; the cur...

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Abstract

The invention discloses bio-ink for 3D printing of a cornea repair material. The bio-ink comprises methacrylamide gelatin, a photoinitiator, collagen and a solvent. The concentration of the methacrylamide gelatin in the bio-ink is 50-150 mg / mL; the concentration of the photoinitiator in the bio-ink is 1-5 mg / mL; and the concentration of the collagen solution in the bio-ink is 0.5-6.0 mg / mL. The invention further discloses a preparation method of the bio-ink for 3D printing of the cornea repair material and a preparation method of the cornea repair material based on the bio-ink for 3D printing of the cornea repair material. The printing precision of the cornea repair material is improved, the mechanical property of the printing stent is improved, and the printing stent has a complete curvature structure, is good in optical permeability and water-containing performance and can fully meet the requirements of growth and proliferation of cornea epithelial cells.

Description

technical field [0001] The invention relates to the field of corneal repair materials, in particular to a bio-ink for 3D printing corneal repair materials, a preparation method thereof, and a preparation method of corneal repair materials. Background technique [0002] Corneal blindness, as the second most common blindness disease in my country, is a major public health problem facing our country. Corneal transplantation is the only way for patients with corneal blindness to regain sight, but corneal transplantation donors are extremely scarce, and there is no corneal repair material product that has been clinically applied on a large scale at home and abroad. key. [0003] The natural cornea is a layered and ordered tissue with curvature and smooth thickness, which makes it have unique refractive properties, transparency, nutrient penetration, and mechanical properties. It has the ability to maintain intraocular pressure and protect the eyeball. Through light and particip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/24A61L27/50B33Y10/00B33Y70/10B33Y80/00
CPCA61L27/222A61L27/24A61L27/50B33Y70/10B33Y10/00B33Y80/00A61L2430/16C08L89/00
Inventor 任力徐颖妮宋文婧
Owner SOUTH CHINA UNIV OF TECH
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