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Cornea repair hydrogel and preparation method and application thereof

A technology for repairing water and hydrogel, which is applied in the field of biomedicine, can solve problems such as restrictions on the wide application of corneal donors, technical safety concerns, etc., and achieve the goal of maintaining cell phenotype and function, simple preparation process, and promoting repair and regeneration Effect

Inactive Publication Date: 2021-07-09
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although 80% of patients can regain sight through corneal transplantation, the severe shortage of corneal donors greatly limits the wide application of this procedure
In addition, similar to other organ transplants, the possible serious complications of corneal donor-derived infections have also raised concerns about the safety of this technique

Method used

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  • Cornea repair hydrogel and preparation method and application thereof
  • Cornea repair hydrogel and preparation method and application thereof
  • Cornea repair hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A corneal repair hydrogel is prepared by the cross-linking reaction of IKK-β specific small molecule inhibitor and silk fibroin, and its preparation method comprises the following steps:

[0025] (1) Preparation of silk fibroin solution:

[0026] a) Degumming: Put 10g of silkworm silk (grade 6A) into 4L of 0.02M sodium carbonate aqueous solution, bathe in 100°C water for 30min, wash with pure water, repeat this process 3 times, remove sericin and leave silk fibroin , drying the silk fibroin at 60°C to obtain 7g of dried silk fibroin for later use;

[0027] b) Dissolution: dissolve the above-mentioned dried silk fibroin in a 9.3M lithium bromide aqueous solution with a mass concentration of 20%, heat at 60°C for 4 hours until the silk fibroin is fully dissolved, and obtain a mixture containing silk fibroin and a small amount of insoluble particles liquid;

[0028] c) Dialysis: dialyze the mixed solution with a regenerated cellulose dialysis bag (molecular weight cut-of...

Embodiment 2

[0033] A corneal repair hydrogel is prepared by the cross-linking reaction of IKK-β-specific small molecule inhibitors and silk fibroin, and its application method includes the following steps:

[0034] (1) Modeling of corneal injury: 6-8 week old male C57BL / 6 mice were taken. After general and local anesthesia, the eyelids were opened with a lid speculum. For the matrix part, the diameter of the incision is about 3mm. Each mouse was modeled with only one eye.

[0035] (2) Application of TPCA-1 hydrogel: A corneal repair hydrogel containing a small molecule inhibitor in Example 1 was covered on the corneal injury created in (1), 10 μL per wound. After the operation, ofloxacin eye drops were applied topically, and the animals were fed with standard animal feed.

[0036] (3) Evaluation of repair effect: On the 1st, 3rd, 5th and 7th day after operation, the repair effect of corneal damage was examined by slit lamp combined with 0.25% sodium fluorescein staining, and the animals...

Embodiment 3

[0038] Dilute the high-concentration silk fibroin solution extracted by the method in Example 1 with sterile pure water to a final concentration of 2% (wt / vol) silk fibroin solution 10mL, add TPCA-1 solution (Selleck) to a final concentration of 10uM . Ultrasound at 80% amplitude for 10 s, placed in an oven and heated at 37° C. for 10 min to obtain a silk fibroin hydrogel complexed with TPCA-1, that is, a corneal repair hydrogel containing small molecule inhibitors.

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Abstract

The invention belongs to the field of biological medicine, and discloses cornea repair hydrogel and a preparation method and application thereof. The cornea repair hydrogel is prepared through a cross-linking reaction of an IKK-beta specific small-molecule inhibitor and silk fibroin, and the preparation method of the cornea repair hydrogel specifically comprises the following steps of 1, using mulberry silk as a raw material, performing degumming, dissolving, dialysis and centrifugation, and obtaining a silk fibroin solution; (2) adding a TPCA-1 solution into the silk fibroin solution obtained in the step (1), and uniformly mixing; and (3) carrying out ultrasonic treatment on the mixed solution of the silk fibroin and the TPCA-1 obtained in the step (2), and carrying out cross-linking reaction at 37 DEG C to obtain the silk fibroin hydrogel compounded with the TPCA-1. The cornea repair hydrogel prepared by the invention is hydrophilic gel with a three-dimensional network structure, has certain adhesion to a cornea injury surface, is suitable for cornea injury repair, is simple in preparation process, does not use additives such as a denaturing agent, a coagulant and a cross-linking agent in the preparation process, and ensures the biological activity of TPCA-1 to the greatest extent.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a corneal repair hydrogel and its preparation method and application. Background technique [0002] Eye diseases have been the focus of the World Health Organization for the past few decades. According to statistics, about 1.3 billion people in the world suffer from some form of visual impairment. Among them, corneal disease caused by trauma and inflammatory infection is one of the common ophthalmic diseases in clinical practice, and it is also the third leading cause of blindness after cataract and glaucoma. In my country, there are currently 4 to 5 million patients with unilateral or bilateral corneal disease, which brings a heavy burden to families and society. [0003] Currently, the only effective clinical treatment for corneal disease is corneal transplantation. Although 80% of patients can recover their sight through keratoplasty, the severe shortage of corneal donors greatly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/52A61L27/54C08J3/075C08J3/24C08L89/00
CPCA61L27/227A61L27/50A61L27/52A61L27/54C08J3/075C08J3/24A61L2430/16A61L2300/412A61L2300/602C08J2389/00C08L89/00
Inventor 张薇陈佳林
Owner SOUTHEAST UNIV
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