Preparation method of silk fibroin tissue engineering scaffold based on near-field electrospinning technology

A tissue engineering scaffold and silk fibroin technology, applied in the field of preparation of silk fibroin tissue engineering scaffolds, can solve the problems that porogens are difficult to completely remove, reduce biomolecular activity, and high porosity of scaffolds, and achieve biocompatibility and high porosity. The effect of good biological activity, high production efficiency and simple preparation method

Inactive Publication Date: 2020-09-18
XI AN JIAOTONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent casting/particle leaching method is to pour the polymer solution containing the porogen into the mold, and remove the porogen after the solvent volatilizes. This method is simple, low in cost, and the size and shape of the formed pores can be controlled. Organic solvents reduce the activity of biomolecules, and if the scaffold is too thick, the porogen in the center will be difficult to remove completely
The freeze-drying method is to sublimate the ice crystals in the material to form an internal porous structure under low temperature and low pressure conditions. The scaffold formed by this method has high porosity, good internal connectivity o

Method used

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  • Preparation method of silk fibroin tissue engineering scaffold based on near-field electrospinning technology
  • Preparation method of silk fibroin tissue engineering scaffold based on near-field electrospinning technology

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Effect test

Embodiment 1

[0030] A silk fibroin tissue engineering scaffold is specifically prepared according to the following steps:

[0031] a. Preparation of silk fibroin aqueous solution: first prepare 2 liters of sodium carbonate solution with a substance concentration of 0.02mol / L, cut 5 grams of silkworm cocoons into pieces, cut them to the size of a coin, and put in a substance concentration of 0.02mol / L Boil in L of sodium carbonate solution for 30 minutes to remove sericin on the surface of silkworm cocoons, take it out, squeeze dry, rinse with ultrapure water for 2 to 3 times, put it in a beaker and stir it fully for 1 hour after rinsing, and change the water 3 times during the rinse , then placed in a fume hood and air-dried at room temperature to obtain silk fibroin fibers. According to the quality of air-dried silk fibroin fibers, the concentration of the prepared substance was a lithium bromide solution of 9.3mol / L, and the prepared lithium bromide solution was poured into the air-dried ...

Embodiment 2

[0037] A silk fibroin tissue engineering scaffold is specifically prepared according to the following steps:

[0038] a. Preparation of silk fibroin aqueous solution: first prepare 2 liters of sodium carbonate solution with a substance concentration of 0.02mol / L, cut 5 grams of silkworm cocoons into pieces, cut them to the size of a coin, and put in a substance concentration of 0.02mol / L Boil in L of sodium carbonate solution for 30 minutes to remove sericin on the surface of silkworm cocoons, take it out, squeeze dry, rinse with ultrapure water for 2 to 3 times, put it in a beaker and stir it fully for 1 hour after rinsing, and change the water 3 times during the rinse , then placed in a fume hood and air-dried at room temperature to obtain silk fibroin fibers, according to the quality of the air-dried silk fibroin fibers, the amount concentration of the preparation substance was a lithium bromide solution of 9.3mol / L, and the prepared lithium bromide solution was poured into ...

Embodiment 3

[0044] A silk fibroin tissue engineering scaffold is specifically prepared according to the following steps:

[0045] a. Preparation of silk fibroin aqueous solution: first prepare 2 liters of sodium carbonate solution with a substance concentration of 0.02mol / L, cut 5 grams of silkworm cocoons into pieces, cut them to the size of a coin, and put in a substance concentration of 0.02mol / L Boil in L of sodium carbonate solution for 30 minutes to remove sericin on the surface of silkworm cocoons, take it out, squeeze dry, rinse with ultrapure water for 2 to 3 times, put it in a beaker and stir it fully for 1 hour after rinsing, and change the water 3 times during the rinse , then placed in a fume hood and air-dried at room temperature to obtain silk fibroin fibers, according to the quality of the air-dried silk fibroin fibers, the amount concentration of the preparation substance was a lithium bromide solution of 9.3mol / L, and the prepared lithium bromide solution was poured into ...

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Abstract

The invention discloses a preparation method of a silk fibroin tissue engineering scaffold based on a near-field electrospinning technology. The preparation method comprises the steps that a regenerated silk fibroin aqueous solution is mixed with a polyoxyethylene aqueous solution, the ratio of the mixed materials is adjusted, the viscosity characteristic of the whole mixture is changed, and a silk fibroin/PEO solution system which can be used for near-field electrospinning is prepared; then the near-field electrospinning printing technology is adopted, printing is carried out according to a scaffold model of a cross-shaped net structure circulating in a periodic cycle, and finally, the preparation of the silk fibroin tissue engineering scaffold is realized through a post-treatment processsuch as chemical modifier soaking. The preparation method is simple, the production efficiency is high, the inherent characteristics of the raw materials are not affected, the porosity of the prepared tissue engineering scaffold is high and adjustable, the pore size is proper, the mechanical property is good, and the specific surface area is large; and the biocompatibility and the biological activity of the scaffold material are good, the extracellular matrix can be simulated well, and the scaffold material is suitable for cell adhesion, proliferation and differentiation.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a method for preparing a silk fibroin tissue engineering scaffold based on near-field electrospinning technology. Background technique [0002] Tissue engineering refers to planting isolated group cells and stem cells in a natural or synthetic cell scaffold that can be gradually degraded and absorbed by the human body after in vitro culture and expansion. This kind of biomaterial scaffold can provide cells with survival The three-dimensional space is conducive to the cells to obtain sufficient nutrients, perform gas exchange, and eliminate waste materials, so that the cells can grow on the prefabricated three-dimensional scaffold, and then the material that adheres to the cells is implanted into the tissue defect, and the biological material is gradually degraded. At the same time, the planted cells continue to proliferate, so as to achieve the purpose of repairing tis...

Claims

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Application Information

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IPC IPC(8): D01F4/00D01F1/10D06M13/144A61L27/26A61L27/56A61L27/58D06M101/10
CPCA61L27/26A61L27/56A61L27/58D01F1/10D01F4/00D06M13/144D06M2101/10C08L89/00C08L71/02
Inventor 王莉王翀张皓罗钰冯学明卢秉恒
Owner XI AN JIAOTONG UNIV
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