22 results about "Sciatic nerve palsy" patented technology

The invention provides application of ursodesoxycholic acid in preparation of a medicine for treating peripheral neuropathy. The ursodesoxycholic acid provided by the invention can promote the recovery of sciatic nerve function indexes after the sciatic nerve of a mouse is cut off, and improve the electroneurographic signal conduction function of an injured part; meanwhile, the UDCA can also relieve oxaliplatin-induced ischiadic nerve signal transduction disorder of mice and related crymodynia sensitive symptoms. In addition, the invention further reveals that the relieving effect of the UDCA on the peripheral neuropathy does not depend on promoting Schwann cell migration. According to the discovery, the application value of the UDCA in treating peripheral neuropathy caused by multiple factors is expanded, and a new strategy is provided for clinical treatment of the UDCA.

The application provides a small animal sciatic nerve magnetic resonance coil and an imaging method, and the method comprises the following steps: filling a high-concentration sugar syrup solution at the bottom of the magnetic resonance coil and performing constant temperature control; placing a small animal in a prone position in the high-concentration sugar syrup solution and discharging air in the gap between the lower limbs and below the abdomen of the small animal; after performing magnetic resonance positioning scanning to determine the main sequence scanning range, starting the main sequence scanning, suppressing the flow tissue signal through a pre-pulse combination, exciting the tissue by using a radio frequency pulse with preset parameters, acquiring echo signals by combining a fast spin echo technology and filling K space, and obtaining scanning original data; performing preprocessing on the scanning original data and accurate segmentation of the sciatic nerve and spinal nerve to obtain a segmentation result; and completing three-dimensional model reconstruction, optimization and multi-modal fusion visualization based on the segmentation result to obtain an image scanning result. The application is suitable for small animals, is accurate in imaging, can effectively suppress various kinds of artifacts, and can be widely applied to sciatic nerve imaging scenarios of small animals.

The application discloses application of a Histatin-1 polypeptide in preparation of a preparation for promoting sciatic nerve regeneration or repair, and an amino acid sequence of the Histatin-1 polypeptide is shown as SEQ ID NO. 1. The Histatin-1 polypeptide can promote expression of a nerve differentiation index, promote recovery of a functional index after sciatic nerve injury, and promote recovery of a histological index after sciatic nerve injury, for example, recovery of myelin structure and morphology. In addition, in combination with gelatin methacrylate (Gel-MA) as a biological scaffold, slow release of the Histatin-1 polypeptide can be realized, continuous repair support can be provided for a nerve injury site, myelin repair effect is further optimized, a more efficient and more durable solution is provided for treatment of sciatic nerve injury, and the application has a wide application prospect in the field of treatment of myelinated nerve injury.

The invention discloses a method for researching the action mechanism of saikoside D on paclitaxel-induced peripheral neuropathy, and relates to the technical field of disease mechanism research and drug development. Comprising the following steps: performing continuous administration through intraperitoneal injection of paclitaxel, and establishing a paclitaxel-induced peripheral neuropathy animal model; a von Frey cellosilk experiment and a cold and hot plate experiment are adopted for behavioral detection, and mechanical pain threshold and cold and hot pain sense changes are evaluated; the morphological structure change of the sciatic nerve myelin sheath is observed through Luxol Fast Blue staining; and the expression level of the myelin sheath basic protein in the sciatic nerve is detected by using immunofluorescence staining and Western blotting technologies. According to the invention, a closed-loop research system is formed from animal model construction to molecular mechanism verification; based on behavioral detection, histological analysis and multiple dimensions of a molecular biological technology, the data reliability is ensured, and the curative effect of saikoside D can be comprehensively evaluated.

The invention provides an application of a nitrogen-containing heterocyclic ring compound in preparation of a medicine for treating peripheral neuropathy. The nitrogen-containing heterocyclic ring compound is SB203580. The peripheral neuropathy refers to diseases or injuries involving a peripheral nervous system, and comprises peripheral nerve trauma, adverse drug reaction and axonal dysfunction caused by one or more pathogenic factors of chemical reagents and diabetes mellitus. Researches show that recovery of a sciatic nerve function index (SFI) after peripheral nerve physical injury (sciatic nerve truncation) of a mouse is remarkably improved, the recovery degree of the signal transduction rate of the injured sciatic nerve is improved, myelin sheath regeneration of the injured sciatic nerve is promoted, and the proportion of abductive myelin sheaths in newly formed myelin sheaths is reduced. The invention provides a new potential medicine for treating the peripheral nerve injury. The structure of the SB203580 is shown in the specification.

This invention discloses a positioning pad for maintaining body position after total hip arthroplasty. The positioning pad includes a first support pad and a second support pad, which are fixedly connected at a 90° angle or rotatably connected via a rotating mechanism. The end of the second support pad away from the first support pad has a lifting adjustment mechanism configured to adjust the angle between the first support pad and the horizontal direction by adjusting the height of the second support pad. The first support pad has a pair of anti-rotation pillows extending along a 30° hip abduction direction. The second support pad, away from the first support pad, has a T-shaped shoe extending along a 30° hip abduction direction. This positioning pad, by providing appropriate support and comfort, ensures that the patient maintains this specific position during recovery, which not only helps reduce sciatic nerve tension and displacement, lowering the incidence of postoperative complications, but also helps promote the patient's rehabilitation process.

Disclosed in the present invention is the use of an anti-Gelsolin3 antibody related to demyelinating Guillain-Barré syndrome. The use mainly comprises the following steps: collecting the serum from a GBS patient and a control individual, performing co-immunoprecipitation with a sciatic nerve protein to obtain an immune complex, separating and identifying the immune complex by using a mass spectrometer to obtain a candidate protein sequence, inserting a candidate protein coding gene into a vector, transfecting cells followed by immunofluorescence verification to determine the localization and expression of the candidate protein, constructing a candidate protein expression plasmid, expressing and purifying a recombinant protein in Escherichia coli, performing co-immunoprecipitation verification on the purified recombinant protein by using the serum from the GBS patient to determine the presence of an anti-Gelsolin3 IgG antibody, further verifying the expression of the anti-Gelsolin3 IgG antibody in the serum from the GBS patient by using a Western blot method, and verifying the binding of the serum from an anti-Gelsolin3 IgG antibody-positive patient to the sciatic nerve by using a single-fiber immunofluorescence method.

The application provides an application of 3-(methylsulfonyl) propionitrile in preparation of a medicine for treating peripheral nerve adhesion diseases, relates to the field of biomedical technology, and is verified through a sciatic nerve adhesion mouse model that 3-(methylsulfonyl) propionitrile can effectively reduce the adhesion degree of nerves and peripheral tissues, and can significantly improve muscle atrophy symptoms; compared with the traumatic and high recurrence rate of surgical treatment, the drug is a non-invasive or minimally invasive treatment, has good patient tolerance, can effectively reduce treatment risks and rehabilitation costs, and has wider clinical applicability. Secondly, the drug plays a treatment effect through a double action mechanism: on the one hand, adhesion formation is inhibited, and nerve compression is released; on the other hand, nerve myelin sheath regeneration can be promoted, myelin sheath structure integrity is improved, nerve conduction velocity is improved, and the synergistic effect of 'adhesion release + nerve repair' is realized. Animal experiments prove that the drug can significantly improve the motion balance ability and gait function of model mice.

The invention discloses a method for improving neuropathic pain, and relates to the technical field of medical treatment. According to the present invention, the number of M2 type macrophages in the SCN is increased by using the low intensity pulse ultrasound (LIPUS) treatment, such that the inflammatory factors of the peripheral nerve and the central nervous system induced by the partial sciatic nerve ligation (PSL) are reduced, and the hypersensitivity reaction down-regulation shows that the neuropathic pain induced by the PSL is effectively improved by using the LIPUS. All data show that LIPUS may be an effective method for treating neuropathic pain.

The invention belongs to the field of preparation of biological nano materials, and discloses an oriented polylactic acid nanofiber membrane with piezoelectric property and a preparation method and application thereof. The preparation method provided by the invention comprises the following steps: S1, adding poly-L-lactic acid and polyethylene oxide into trifluoroethanol, stirring and dissolving, and adding magnesium silicide to obtain a spinning solution; and S2, carrying out electrostatic spinning on the spinning solution, collecting an oriented fiber membrane, and carrying out vacuum drying to obtain the oriented polylactic acid nanofiber membrane. The oriented polylactic acid nanofiber membrane prepared by the preparation method disclosed by the invention has excellent piezoelectric property and high orientation, can release hydrogen when encountering water, and can be applied to piezoelectric sensors and ischium application repair materials.

The application provides application of flufenamic acid in preparation of a medicine for treating peripheral nerve adhesion diseases, and relates to the technical field of biomedicine, and the technical scheme is characterized in that: application of flufenamic acid (2-(3-trifluoromethyl anilino) benzoic acid) in preparation of a medicine for treating peripheral nerve adhesion diseases, the peripheral nerve adhesion diseases including sciatic nerve adhesion. The application provides application of flufenamic acid in preparation of a medicine for treating peripheral nerve adhesion diseases, and the application proves that 2-(3-trifluoromethyl anilino) benzoic acid (flufenamic acid, FFA, CAS: 530-78-9) can effectively promote the motor balance ability and nerve conduction rate of a mouse, promote recovery of adhesion nerves, and has a positive treatment effect on peripheral nerve adhesion diseases.

The invention discloses a microneedle nerve conduit containing extracellular vesicles and also discloses a preparation method of the nerve conduit, and the preparation method comprises the following steps: selecting a healthy adult male mouse, making an incision in the hip of the mouse, and cutting off sciatic nerves at the far end of the ischial notch, then separating the gastrocnemius muscle tissue, and extracting acute denervation skeletal muscle source extracellular vesicles Mu-EVs from the tissue; the preparation method comprises the following steps: preparing a GelMA hydrogel pre-polymerization solution by using a methacrylated monomer and a photoinitiator, and adding Mu-EVs into the pre-polymerization solution to obtain Mu-EVs-containing GelMA hydrogel; preparing the hydrogel into a microneedle patch; and preparing the microneedle patch into the microneedle nerve conduit. According to the nerve conduit disclosed by the invention, through the synergistic effect of physical support and long-acting drug delivery of the conduit, the acute denervation skeletal muscle derived extracellular vesicles are continuously released, the metabolism of damaged neurons is activated, the regeneration of axons is accelerated, and the repair effect of peripheral nerve injury is improved.

The invention provides the application of SCD1 in preparing the medicine for treating the nerve myelin sheath injury diseases, the application reveals that SCD1 serves as a brand-new target spot for treating the sciatic nerve myelin sheath injury diseases for the first time, the target spot blank of existing targeted lipid metabolism regulation myelin sheath regeneration medicine is filled, and all-around repairing can be achieved from the molecule, form and function level; on one hand, the SCD1 can up-regulate expression of genes such as PMP22 and MPZ and promote myelin sheath synthesis from the source, and on the other hand, the SCD1 can also increase the myelin sheath thickness, reduce the number of non-marrow nerves and improve the structural integrity and stability of regenerated myelin sheaths; meanwhile, the SCD1 can prolong the dwell time of the rotating rod, accelerate the nerve conduction speed and improve dyskinesia and nerve conduction abnormity after sciatic nerve injury; besides, regulation of endogenous lipid metabolism is taken as a core mechanism, an intervention mode is safe and controllable, an experimental system is mature and repeatable, and an efficient, stable and safe new strategy is provided for clinical treatment of sciatic nerve myelin sheath injury.

The application discloses a preparation method of a microneedle patch loaded with extracellular vesicles of damaged nerve tissue, extracts extracellular vesicles after sciatic nerve damage; the obtained extracellular vesicles are modified to introduce polymerizable double bonds; a hydrogel solution loaded with the extracellular vesicles is prepared as a microneedle tip material; the hydrogel solution is prepared as a microneedle substrate material; and finally, a tip-substrate separation type microneedle patch loaded with extracellular vesicles from damaged nerve tissue is prepared. The application also provides the microneedle patch loaded with extracellular vesicles from damaged nerve tissue prepared by the above method and application thereof. The microneedle patch, the preparation method and the application thereof solve the problem that the existing nerve repair strategies based on extracellular vesicles mainly focus on extracellular vesicles of specific cell sources cultured in vitro, and cannot completely simulate the complex signal network of the synergistic effect of various cells in the microenvironment of in-vivo nerve tissue repair after damage.

A double-incision surgical navigation method for acetabulum revision comprises a planning process, including digital planning of incisions and tunnels before an operation and planning of a main incision: on a three-dimensional coordinate system of a pelvis, carrying out navigation on the three-dimensional coordinate system of the pelvis, and carrying out navigation on the three-dimensional coordinate system of the pelvis; the method comprises the following steps: simulating a main incision path along a hip large muscle fiber direction and a thighbone axis, ensuring that the path can expose an acetabular margin, a residual acetabular cup and sciatic nerves, marking the walking of the sciatic nerves in a three-dimensional coordinate system of the pelvis as an absolute forbidden zone in an operation, and positioning an auxiliary incision: positioning a gluteus column bullseye, and on the three-dimensional coordinate system of the pelvis, positioning an auxiliary incision on the three-dimensional coordinate system of the pelvis; the method comprises the following steps: defining a gluteus column bone surface area used for fixing a reinforcing block, acquiring a walking model of a suprahip nerve and blood vessel bundle, avoiding an incision and an approach which are positioned at the rear lower part of the suprahip nerve and blood vessel bundle, virtually constructing a subcutaneous tunnel, drawing a smooth 3D curve pointing to the rear upper part of an acetabulum in a main incision from the deep part of an auxiliary incision, according to the size of the winged reinforcing block including the width of the fixed wing, the minimum diameter needed by the tunnel is determined, and it is ensured that the implant can pass through smoothly.

The utility model discloses a deformed correction cushion block, including cushion body, the cushion body upside is equipped with first recess and second recess, the second recess is connected with first recess, be provided with first soft pad in the first recess, a plurality of strip grooves are equipped on the soft pad upside, be provided with second soft pad in the second recess, the first soft pad covers second soft pad, and the second soft pad includes soft shell, and the soft shell is filled with fluid. First recess is equipped with a soft pad with strip groove, and the second recess is equipped with a soft shell filled with fluid, and the both are overlapped and placed, so when the human body sits on the cushion block, their hip sciatic nerve just falls in the second soft pad right above, and the second soft pad filled with fluid can adjust according to the weight and change of human body sitting posture, provides the equal support force of left and right sides, effectively alleviates the body discomfort and pain due to the pelvic height inconsistency, makes the body keep in the comfortable, balanced state.

The invention provides application of otolith protein 1 (OTOP1) in preparation of drugs for treating sciatic nerve myelin injury diseases, relates to the technical field of biomedicine, systematically verifies the core effect of the OTOP1 in sciatic nerve myelin injury repair for the first time, defines the application value of the OTOP1 as a potential target spot for treating the sciatic nerve myelin injury diseases, and provides an application of the OTOP1 in preparation of drugs for treating the sciatic nerve myelin injury diseases. The research blank of OTOP1 in the field of peripheral nerve myelin sheath injury repair is filled. The research result provides a sufficient experimental basis for application of OTOP1 in preparation of drugs for treating sciatic nerve myelin sheath injury diseases, also provides a brand new molecular target and treatment thought for clinical treatment of peripheral nerve myelin sheath injury diseases, and lays an important experimental foundation for research and development and clinical transformation of subsequent related drugs. The method has important guiding significance on research and clinical application in the field of peripheral nerve injury repair.

The invention provides an application of MF-438 in preparation of drugs for treating peripheral nerve adhesion diseases, relates to the technical field of biomedicine, and aims to fill the technical blank of clinical lack of targeted small molecule drugs for treating peripheral nerve adhesion. A verification experiment also proves that the MF-438 can obviously reduce the expression of proinflammatory factors such as IL-1alpha, IL-1beta and TNF-alpha, effectively inhibit the excessive inflammatory response of a nerve adhesion part and create a stable local microenvironment for nerve repair; meanwhile, the compound can effectively protect the sciatic nerve myelin sheath structure, increase the myelin sheath thickness and maintain the myelin sheath integrity, and the nerve protection and repair effects are directly achieved; in addition, the MF-438 has good cell permeability and oral bioavailability, the administration mode is simple and convenient, the action effect is clear, and the experimental safety is good, so that the MF-438 has higher clinical transformation and industrialization potential, and a safe, efficient and clear-mechanism brand new treatment strategy is provided for peripheral nerve adhesion diseases.

The application discloses application of an ACSS2 gene in preparation of a medicine for treating or repairing nerve injury, and belongs to the technical field of biological medicines. The application finds, through transcriptome sequencing results of spinal cord injury and sciatic nerve pinch injury, that the expression of ACSS2 presents a gradually decreasing trend after spinal cord injury and sciatic nerve pinch injury, and then the application adopts an adeno-associated virus to overexpress ACSS2 in neuron primary cells, and the result shows that the length of neuron axons is significantly increased after overexpression of ACSS2. Therefore, a substance for promoting the expression of the ACSS2 gene can realize treatment or repair of nerve injury by promoting the growth of neuron axons.

The application provides a smooth muscle cell extracellular matrix modified functionalized nerve conduit and a preparation method and use thereof, and belongs to the field of biological tissue materials. The application inoculates smooth muscle cells on the surface of a material, and obtains a functionalized nerve conduit through smooth muscle cell derived extracellular matrix modification. The nerve conduit significantly promotes the axon regeneration of dorsal root ganglion and PC12, and promotes angiogenesis by inducing Schwann cell migration and VEGF secretion, thereby providing a guiding "bridge" for the newly generated axon, reconnecting the cut nerves, repairing the large segment defect sciatic nerve, improving the functional innervation and muscle function after nerve regeneration, and having a good application prospect.

The invention discloses a tissue-derived extracellular vesicle for treating peripheral nerve injury, and also discloses a preparation method of the extracellular vesicle, and the preparation method comprises the following steps: selecting a healthy adult male mouse, making an incision in the hip, then cutting off sciatic nerves at the far end of the ischial notch and excising nerve segments, and constructing an acute denervation mouse model; separating the gastrocnemius muscle tissue of the modeled mouse, cutting the tissue into fragments, digesting the tissue, collecting the supernatant, filtering, centrifuging, collecting the supernatant to remove impurities, performing ultracentrifugation, and collecting the precipitate to obtain the denervation acute skeletal muscle-derived extracellular vesicles Mu-EVs. The extracellular vesicles are rich in energy metabolism regulation enzyme, and can realize accurate and efficient regulation of energy metabolism of damaged neurons, so as to accelerate axonal regeneration.

The invention discloses a comprehensive testing device for sciatic nerve trunks, motor nerves and skeletal muscles. The comprehensive testing device comprises a specimen bearing assembly composed of PU plates, an electrophysiological signal collecting and stimulating assembly composed of acupuncture needles and miniature alligator clips, and a muscular tension testing assembly composed of a support and a tension transducer. The PU plate can fix an in-vitro spine-spinal nerve-sciatic nerve trunk-tibial nerve-gastrocnemius muscle integrated active specimen of a frog, and the acupuncture needle can puncture an adventitia and is arranged between nerve fascia membranes or puncture an adventitia of skeletal muscle and is arranged between muscular fascia membranes and is connected with a biological function data analyzer, so that electrical stimulation application and electric signal and electromyographic signal acquisition are realized, and the biological function data analyzer is connected with a biological function data analyzer. The tension transducer is connected with the specimen and synchronously collects muscular tension signals. Neural and muscle testing functions are integrated, the use amount of experimental animals is reduced, the signal-to-noise ratio of signals is increased through acupuncture needle puncture type collection, the PU plate is flexible in adaptation, the electrode distance is adjustable, and neural electrophysiological signals and muscular tension signals can be synchronously obtained.