54 results about "Sciatic nerve palsy" patented technology

The invention discloses a sacral plexus and sciatic nerve ultrasound image recognition and puncture guide method and device, and the method comprises the steps: inputting a sacral plexus and sciatic nerve ultrasound image into a target image classification model, and carrying out the classification of the sacral plexus and sciatic nerve ultrasound image, and obtaining the image types of the sacral plexus and sciatic nerve ultrasound image; determining a region category set based on the image categories of the sacral plexus and sciatic nerve ultrasound images; inputting the sacral plexus and sciatic nerve ultrasonic image into a target image segmentation model and carrying out image segmentation on the sacral plexus and sciatic nerve ultrasonic image to obtain a plurality of tissue segmentation regions and region categories of the plurality of tissue segmentation regions on the sacral plexus and sciatic nerve ultrasonic image; and sending prompt information based on the plurality of tissue segmentation regions and the region categories of the plurality of tissue segmentation regions. According to the method, the accuracy of sacral plexus and sciatic nerve ultrasonic image recognition and puncture guiding can be improved.

The invention discloses an SD rat neuropathic pain model dorsal root ganglion cell extraction method, and relates to the technical field of dorsal root ganglion cell extraction. In order to facilitate further research of physiological functions of dorsal root ganglion cells in in-vitro cells; comprising the following steps: selecting an SD (Sprague Dawley) rat as an experimental animal, carrying out sciatic nerve branch selective ligation (SNI), ligating tibial nerves and common peroneal nerves in sciatic nerves, keeping the completeness of the peroneal nerves, and observing the touch sensitivity change of the outer side area of the rat claw after the operation to verify that the model is successfully constructed. According to the method, the extraction efficiency of the dorsal root ganglion cells is effectively improved through the cell treatment steps such as surgical operation under the sterile condition, reasonable use of digestive enzymes and centrifugation and resuspension, so that the experiment time and cost are saved, the smooth proceeding of subsequent experiments is ensured, and the neuropathic pain model is established, so that the development of the neuropathic pain model is promoted. And a basis is provided for subsequent extraction and research of the dorsal root ganglion cells.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine preparation for treating neuropathic pain and a preparation method thereof. The traditional Chinese medicine preparation for treating neuropathic pain comprises the following raw materials in parts by weight: 18-25 parts of cassia twig, 13-17 parts of red thick shell, 12-15 parts of loofah sponge, 8-12 parts of elm leaf, 10-14 parts of melia toosendan, 5-9 parts of eucommia ulmoides, 3-8 parts of angelica sinensis, 1-5 parts of dendrobe, 1-3 parts of fresh ginger and 1-3 parts of liquorice. According to the traditional Chinese medicine preparation provided by the invention, all the medicines are combined, both symptoms and root causes are taken into consideration, the effects of clearing and activating the channels and collaterals, promoting blood circulation to remove blood stasis and removing blood stasis to relieve pain are achieved, and neuropathic pain caused by sciatic nerve chronic compression injury can be effectively treated.

The invention discloses a sciatic nerve pathway etiological screening method and screening system, and belongs to the technical field of medical image processing. The method comprises the following steps: in combination with an MRI image, constructing a space sequence perception deep learning model by utilizing space sequence patch embedding, MRI sequence coding, a space perception module based on sequence space attention, a space-sequence encoder and a classification head, and training to obtain a trained network model; using the trained model to test an unlabeled image, and realizing binary classification of a high-signal area and a background area; and the primary screening of sciatic nerve pathway pathogenesis is realized by visualizing the classification result. According to the method, accurate screening of sciatic nerve pathway lesions is achieved with high efficiency and high accuracy, excellent robustness and generalization ability are shown at the same time, noise interference and data distribution differences can be effectively coped with, and reliable technical support is provided for early recognition and diagnosis of sciatic nerve lesions.

The invention relates to an artificial intelligence-based decision support method for determining the sciatic nerve prior to intramuscular injection and enabling safe injection.

The invention provides an application of flufenamic acid in preparation of a medicine for treating peripheral nerve adhesion diseases, relates to the technical field of biomedicine, and adopts the technical scheme that the application of the flufenamic acid (2-(3-trifluoromethylaniline) benzoic acid) in preparation of the medicine for treating the peripheral nerve adhesion diseases is provided. The peripheral nerve adhesion diseases comprise sciatic nerve adhesion. The invention provides an application of flufenamic acid in preparation of drugs for treating peripheral nerve adhesion diseases, and a specific verification experiment proves that 2-(3-trifluoromethylanilino) benzoic acid (flufenamic acid, FFA, CAS: 530-78-9) can effectively promote the movement balance ability and nerve conduction rate of mice, promote the recovery of adhered nerves, and can be used for preparing drugs for treating peripheral nerve adhesion diseases. The positive treatment effect on peripheral nerve adhesion diseases is achieved.

The invention provides a functional nerve conduit modified by a smooth muscle extracellular matrix as well as a preparation method and application of the functional nerve conduit, and belongs to the field of biological tissue materials. Smooth muscle cells are inoculated to the surface of a material, the functional nerve conduit is prepared through smooth muscle cell-derived extracellular matrix modification, the nerve conduit remarkably promotes dorsal root ganglion and PC12 axon regeneration, angiogenesis is promoted by inducing Schwann cell migration and VEGF secretion, a guiding bridge is provided for new axon, and the nerve conduit has the advantages that the nerve conduit has a good application prospect. The cut-off nerve is reconnected, the large-segment defective sciatic nerve is repaired, the function control and the muscle function after nerve regeneration are improved, and good application prospects are achieved.

The invention provides application of miR-21 in preparation of a reagent for promoting BMSCs (bone marrow mesenchymal stem cells) differentiation and a medicine for repairing peripheral nerve injury, and belongs to the technical field of biomedicine.The research finds that overexpression of miR-21 in BMSCs can promote BMSCs to be differentiated into SCs-like cells, and miR-21 can improve the efficiency of BMSCs in vivo to be differentiated into the SCs-like cells. In addition, the invention also finds that the BMSCs overexpressing miR-21 can significantly promote PNI posterior process regeneration and myelin sheath formation, thereby improving sciatic nerve motor function recovery and target muscle atrophy; the compound can be applied to preparation of drugs for repairing peripheral nerve injury.

The present invention relates to the therapeutic intervention of chronic pain, in particular to the treatment of chronic pain using selected probiotic strains having analgesic properties, in particular three probiotics, Roseburia intestinae, Lacnospiraceae bacteria and Roseburia hominis, and a sterile secretion filtrate of Roseburia intestinae, which when administered in sufficient doses exhibit significant analgesic properties in the sciatic nerve branch selective injury (SNI) model.

The invention discloses oligopeptide and application thereof in preparation of a medicine for treating and / or relieving pain. The amino acid sequence of the oligopeptide disclosed by the invention comprises a derivative sequence formed by additionally arranging 1-3 amino acids at the N end and / or C end of the amino acid sequence shown as Tyr-Pro-Arg, especially Tyr-Pro-Arg-Arg (YPRR, YR4), and a gastrointestinal tract digestion product Tyr-Pro-Arg (YPR, YR3) of the oligopeptide. The oligopeptide not only can relieve acute pain induced by a mouse thermal drifting model, but also can relieve chronic pain induced by a rat sciatic nerve chronic compression injury model and a tibia bone cancer pain model, and meanwhile, the oligopeptide obviously inhibits CCI-induced spinal astrocyte, microglia and neuron activation; therefore, the compound has important development and application values in the aspects of central sensitization inhibition and analgesia.

The invention provides application of ursodesoxycholic acid in preparation of a medicine for treating peripheral neuropathy. The ursodesoxycholic acid provided by the invention can promote the recovery of sciatic nerve function indexes after the sciatic nerve of a mouse is cut off, and improve the electroneurographic signal conduction function of an injured part; meanwhile, the UDCA can also relieve oxaliplatin-induced ischiadic nerve signal transduction disorder of mice and related crymodynia sensitive symptoms. In addition, the invention further reveals that the relieving effect of the UDCA on the peripheral neuropathy does not depend on promoting Schwann cell migration. According to the discovery, the application value of the UDCA in treating peripheral neuropathy caused by multiple factors is expanded, and a new strategy is provided for clinical treatment of the UDCA.

The invention discloses application of isotoosendanin in preparation of a medicine for relieving neuropathic pain and application of TRPV1 and Nav1.7 as medicine targets of the isotoosendanin, and relates to the technical field of biological medicine. The medicine prepared from the isotoosendanin can inhibit the expression of dorsal root ganglion (DRG) neurons TRPV1 and Nav1.7 protein; the medicine prepared from the isotoosendanin can inhibit the reduction of mechanical pain threshold and crymodynia threshold; the neuropathic pain comprises pain after selective injury of sciatic nerve branches; the TRPV1 and Nav1.7 are used as drug targets to be applied to preparation of the neuropathic pain relieving drug taking the isotoosendanin as an effective component. The isotoosendanin plays a role in relieving neuropathic pain, and the isotoosendanin can be used for inhibiting the expression of TRPV1 and Nav1.7; the isotoosendanin can be used for treating neuropathic pain by inhibiting expression of TRPV1 and Nav1.7 channel proteins, and a new thought is provided for preparing the neuropathic pain medicine.

The application belongs to the technical field of animal model construction, and discloses a method for establishing a tumor-bearing animal model for an OCT system, wherein a SD rat supratentorial brain parenchymal glioma model, a SD rat chiasma glioma model and a SD rat brain stem glioma model are constructed by using a C6 cell line; a rat sciatic nerve sheath tumor model and a rat trigeminal nerve RT4 tumor model are constructed by using an RT4 cell line to obtain a SD rat RT4 tumor cell animal model; and a BALB / c nude mouse U87MG tumor cell animal model and a BALB / c nude mouse IOMM-Lee tumor cell animal model are constructed by using a U87MG cell line and an IOMM-Lee cell line, respectively. The application successfully constructs a rat brain glioma model, a chiasma glioma model, a trigeminal nerve sheath tumor model, a sciatic nerve sheath tumor model, a nude mouse brain glioma model and a nude mouse sciatic nerve meningioma model.

The application provides a small animal sciatic nerve magnetic resonance coil and an imaging method, and the method comprises the following steps: filling a high-concentration sugar syrup solution at the bottom of the magnetic resonance coil and performing constant temperature control; placing a small animal in a prone position in the high-concentration sugar syrup solution and discharging air in the gap between the lower limbs and below the abdomen of the small animal; after performing magnetic resonance positioning scanning to determine the main sequence scanning range, starting the main sequence scanning, suppressing the flow tissue signal through a pre-pulse combination, exciting the tissue by using a radio frequency pulse with preset parameters, acquiring echo signals by combining a fast spin echo technology and filling K space, and obtaining scanning original data; performing preprocessing on the scanning original data and accurate segmentation of the sciatic nerve and spinal nerve to obtain a segmentation result; and completing three-dimensional model reconstruction, optimization and multi-modal fusion visualization based on the segmentation result to obtain an image scanning result. The application is suitable for small animals, is accurate in imaging, can effectively suppress various kinds of artifacts, and can be widely applied to sciatic nerve imaging scenarios of small animals.

The present invention provides a method for constructing an animal model of neuropathic pain caused by thermal injury, the method comprising: performing radiofrequency thermocoagulation on the sciatic nerve of an adult male SD rat; the radiofrequency thermocoagulation method is a bipolar radiofrequency mode, the position is 0.5 cm and 1 cm from the bifurcation of the rat sciatic nerve to the head end, and the radiofrequency parameters are as follows: temperature 65°C, pulse rate 2Hz, pulse width 20ms, duration 1min, and two radiofrequency thermocoagulation treatments are performed, with an interval of 5 minutes. The present invention constructs an animal model of neuropathic pain caused by thermal injury, the sensitivity to mechanical pain stimulation and the sensitivity to thermal pain stimulation are both increased, the sciatic nerve is damaged by light microscopy, the expression of Nav1.8mRNA is increased in the dorsal root ganglion, and a sequence of changes after radiofrequency thermocoagulation is consistent with the characteristics of neuropathic pain.

The application discloses application of a Histatin-1 polypeptide in preparation of a preparation for promoting sciatic nerve regeneration or repair, and an amino acid sequence of the Histatin-1 polypeptide is shown as SEQ ID NO. 1. The Histatin-1 polypeptide can promote expression of a nerve differentiation index, promote recovery of a functional index after sciatic nerve injury, and promote recovery of a histological index after sciatic nerve injury, for example, recovery of myelin structure and morphology. In addition, in combination with gelatin methacrylate (Gel-MA) as a biological scaffold, slow release of the Histatin-1 polypeptide can be realized, continuous repair support can be provided for a nerve injury site, myelin repair effect is further optimized, a more efficient and more durable solution is provided for treatment of sciatic nerve injury, and the application has a wide application prospect in the field of treatment of myelinated nerve injury.

The invention discloses a method for researching the action mechanism of saikoside D on paclitaxel-induced peripheral neuropathy, and relates to the technical field of disease mechanism research and drug development. Comprising the following steps: performing continuous administration through intraperitoneal injection of paclitaxel, and establishing a paclitaxel-induced peripheral neuropathy animal model; a von Frey cellosilk experiment and a cold and hot plate experiment are adopted for behavioral detection, and mechanical pain threshold and cold and hot pain sense changes are evaluated; the morphological structure change of the sciatic nerve myelin sheath is observed through Luxol Fast Blue staining; and the expression level of the myelin sheath basic protein in the sciatic nerve is detected by using immunofluorescence staining and Western blotting technologies. According to the invention, a closed-loop research system is formed from animal model construction to molecular mechanism verification; based on behavioral detection, histological analysis and multiple dimensions of a molecular biological technology, the data reliability is ensured, and the curative effect of saikoside D can be comprehensively evaluated.

The invention relates to the technical field of biomedicine, in particular to a method for promoting formation of nerve fasciae and repair and regeneration after sciatic nerve defect by using small extracellular vesicles (hfNCSCs-sEVs) derived from hair follicle neural crest stem cells and application. According to the application disclosed by the invention, the effects of the hfNCSCs-sEVs on proliferation, migration, tube formation and barrier formation of nerve fascia cells in vitro are defined; the effect of hfNCSCs-sEVs on tissue regeneration and function recovery after rat sciatic nerve defect is defined, the hfNCSCs-sEVs inhibits expression of Smad7 in nerve tract membrane cells by transferring miR-21-5p, activates a TGF-beta / Smad signal channel, up-regulates expression of HAS2, promotes proliferation and migration of HAS2 and promotes expression of tight junction protein thereafter, and the hfNCSCs-sEVs can be used for treating rat sciatic nerve defect. And a new view angle and theoretical support are provided for repair and regeneration of sciatic nerve injury.

1. The name of the product of this design: Electric elliptical stepper (T1). 2. Purpose of the product with this appearance design: The electric stepper used for rehabilitation exercise can exercise and stimulate the sciatic nerve to regulate and enhance the endurance and strength of the waist muscles while protecting the safety of the joints. It can also stimulate the buttocks, thighs, side waist and lower abdomen to achieve the effect of body shaping. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: Stereoscopic diagram 1.

The invention provides an application of a nitrogen-containing heterocyclic ring compound in preparation of a medicine for treating peripheral neuropathy. The nitrogen-containing heterocyclic ring compound is SB203580. The peripheral neuropathy refers to diseases or injuries involving a peripheral nervous system, and comprises peripheral nerve trauma, adverse drug reaction and axonal dysfunction caused by one or more pathogenic factors of chemical reagents and diabetes mellitus. Researches show that recovery of a sciatic nerve function index (SFI) after peripheral nerve physical injury (sciatic nerve truncation) of a mouse is remarkably improved, the recovery degree of the signal transduction rate of the injured sciatic nerve is improved, myelin sheath regeneration of the injured sciatic nerve is promoted, and the proportion of abductive myelin sheaths in newly formed myelin sheaths is reduced. The invention provides a new potential medicine for treating the peripheral nerve injury. The structure of the SB203580 is shown in the specification.

This invention discloses a positioning pad for maintaining body position after total hip arthroplasty. The positioning pad includes a first support pad and a second support pad, which are fixedly connected at a 90° angle or rotatably connected via a rotating mechanism. The end of the second support pad away from the first support pad has a lifting adjustment mechanism configured to adjust the angle between the first support pad and the horizontal direction by adjusting the height of the second support pad. The first support pad has a pair of anti-rotation pillows extending along a 30° hip abduction direction. The second support pad, away from the first support pad, has a T-shaped shoe extending along a 30° hip abduction direction. This positioning pad, by providing appropriate support and comfort, ensures that the patient maintains this specific position during recovery, which not only helps reduce sciatic nerve tension and displacement, lowering the incidence of postoperative complications, but also helps promote the patient's rehabilitation process.

Disclosed in the present invention is the use of an anti-Gelsolin3 antibody related to demyelinating Guillain-Barré syndrome. The use mainly comprises the following steps: collecting the serum from a GBS patient and a control individual, performing co-immunoprecipitation with a sciatic nerve protein to obtain an immune complex, separating and identifying the immune complex by using a mass spectrometer to obtain a candidate protein sequence, inserting a candidate protein coding gene into a vector, transfecting cells followed by immunofluorescence verification to determine the localization and expression of the candidate protein, constructing a candidate protein expression plasmid, expressing and purifying a recombinant protein in Escherichia coli, performing co-immunoprecipitation verification on the purified recombinant protein by using the serum from the GBS patient to determine the presence of an anti-Gelsolin3 IgG antibody, further verifying the expression of the anti-Gelsolin3 IgG antibody in the serum from the GBS patient by using a Western blot method, and verifying the binding of the serum from an anti-Gelsolin3 IgG antibody-positive patient to the sciatic nerve by using a single-fiber immunofluorescence method.

The invention discloses a method for analyzing the action mechanism of isotoosendanin for relieving neuropathic pain based on network pharmacology and application, and relates to the technical field of biological medicine. The method for analyzing the action mechanism of isotoosendanin for relieving neuropathic pain based on network pharmacology comprises the following steps: S1, obtaining isotoosendanin and neuropathic pain targets; S2, intersection of isotoosendanin and neuropathic pain targets; S3, construction of an interaction network of isotoosendanin and neuropathic pain targets; S4, GO annotation and KEGG enrichment analysis; molecular docking: by adopting the method provided by the invention, related information can be comprehensively collected, and the mutual relation between the isotoosendanin and neuropathic pain related targets can be intuitively displayed; the isotoosendanin can reduce the expression level of DRG neuron TRPV1 and Nav1.7 channel protein, improve sciatic nerve branch selective injury mechanical abnormal pain and cold hypersensitivity, and play a role in easing pain.

The application provides an application of 3-(methylsulfonyl) propionitrile in preparation of a medicine for treating peripheral nerve adhesion diseases, relates to the field of biomedical technology, and is verified through a sciatic nerve adhesion mouse model that 3-(methylsulfonyl) propionitrile can effectively reduce the adhesion degree of nerves and peripheral tissues, and can significantly improve muscle atrophy symptoms; compared with the traumatic and high recurrence rate of surgical treatment, the drug is a non-invasive or minimally invasive treatment, has good patient tolerance, can effectively reduce treatment risks and rehabilitation costs, and has wider clinical applicability. Secondly, the drug plays a treatment effect through a double action mechanism: on the one hand, adhesion formation is inhibited, and nerve compression is released; on the other hand, nerve myelin sheath regeneration can be promoted, myelin sheath structure integrity is improved, nerve conduction velocity is improved, and the synergistic effect of 'adhesion release + nerve repair' is realized. Animal experiments prove that the drug can significantly improve the motion balance ability and gait function of model mice.

The invention belongs to the field of biological medicine, and particularly relates to application of a Caspr4 inhibitor in preparation of axonal injury repair medicine. It is found that after the sciatic nerve axon of a mouse is damaged, the expression quantity of Caspr4 on a corresponding DRG neuron is remarkably reduced, and regeneration of the damaged axon is promoted by inhibiting the activity of Caspr4 in DRG and RGC neuron, which indicates that Caspr4 plays an important role in regulation and control of regeneration of central and peripheral axons, regeneration of the damaged axon can be effectively promoted by adopting an effective Caspr4 inhibitor, and the activity of Caspr4 is inhibited. The compound is expected to become a medicine for treating axonal injury.

The present invention discloses a composition comprising a carrier and either sodium chloride or potassium chloride for use in resolving fascial shortening by applying the composition to the skin in the region of the trigeminal nerve, the fascial nerve, the median nerve, the sciatic nerve, or a combination of at least two of these regions.

The invention discloses a method for improving neuropathic pain, and relates to the technical field of medical treatment. According to the present invention, the number of M2 type macrophages in the SCN is increased by using the low intensity pulse ultrasound (LIPUS) treatment, such that the inflammatory factors of the peripheral nerve and the central nervous system induced by the partial sciatic nerve ligation (PSL) are reduced, and the hypersensitivity reaction down-regulation shows that the neuropathic pain induced by the PSL is effectively improved by using the LIPUS. All data show that LIPUS may be an effective method for treating neuropathic pain.

The invention belongs to the field of preparation of biological nano materials, and discloses an oriented polylactic acid nanofiber membrane with piezoelectric property and a preparation method and application thereof. The preparation method provided by the invention comprises the following steps: S1, adding poly-L-lactic acid and polyethylene oxide into trifluoroethanol, stirring and dissolving, and adding magnesium silicide to obtain a spinning solution; and S2, carrying out electrostatic spinning on the spinning solution, collecting an oriented fiber membrane, and carrying out vacuum drying to obtain the oriented polylactic acid nanofiber membrane. The oriented polylactic acid nanofiber membrane prepared by the preparation method disclosed by the invention has excellent piezoelectric property and high orientation, can release hydrogen when encountering water, and can be applied to piezoelectric sensors and ischium application repair materials.

The invention discloses an in-vivo and in-vitro communication method and system based on a biological neural pathway, and aims to solve the problems of poor biocompatibility and complex operation of in-vivo and in-vitro communication in the prior art. The in-vivo and in-vitro communication method based on the biological neural pathway comprises the steps that information needing to be transmitted is modulated through a transmitting end, and bullfrog sciatic nerves are electrically stimulated through electrodes to generate composite action potential (CAPs); the composite action potential is conducted to nerve endings along sciatic nerve fibers, so that neurotransmitters are promoted to be released, end plate potential (EPP) is triggered, muscle contraction is caused, and surface electromyogram signals (sEMG) are generated; and the receiving end detects the change of the sEMG signal through the surface electrode, and amplifies, filters and judges the sEMG signal to obtain information sent by the transmitting end. According to the method, in-vivo and in-vitro information transmission is achieved through the biological neural channel, and the method has the advantages of being high in biological compatibility, natural in communication mode and small in trauma.

The invention discloses a verification method of a rat neuropathic pain model, and relates to the field of clinical medicine. Comprising the following steps: animal preparation: selecting healthy adult rats, and putting the rats in a laboratory environment for adaptive feeding; model construction: constructing a sciatic nerve branch selective injury model; the pain behavioral test comprises a foot sole hot stabbing pain experiment, a mechanical pain sense test and a cold pain sense test; data analysis: performing data analysis according to a behavioral test result; and model verification: verifying whether the construction of the model is successful or not by observing the behavioral performance of the rat, comparing the symptoms of the model rat with the symptoms of a human neuropathic pain patient, and evaluating the clinical similarity of the model. According to the method, the neuropathic pain model is successfully induced and verified through SNI model surgical operation and hot plate testing, and the method has high reliability and repeatability and provides powerful support for research of neuropathic pain.