Method for producing recombined streptokinase

Abstract

This invention describes a process for producing a streptokinase r-SK, comprising the steps of: a) under appropriate expression conditions, cultivating colibacillus engineering cells that carry an expression carrier selected from the following group: the carrier that contains arabinose-induced promoters, or the carrier that not only contains arabinose-induced promoters but also possess the function of directing the targeting proteins to interstitial cells. The coding sequence of the streptokinase is inserted into the expression carrier in order to express soluble and highly active r-SK; b) separating and purifying the step a) wherein said r-SK. In the process of this invention, the expression amount is high, and the separation and purification steps are simple. This invention also describes corresponding expression carriers and engineering cells.

Description

technical field

[0001] The invention relates to a production method of recombinant streptokinase (Recombinant Streptokinase), as well as expression vectors and host cells used in the method. Background technique

[0002] Under normal physiological conditions, the coagulation system, anticoagulation system and fibrinolytic system in the blood maintain a dynamic balance, which not only ensures the potential coagulability of the blood but also ensures the fluid state of the blood at all times. But in some pathological conditions, the above balance will be broken. Damage to the vascular endothelium may occur in some parts of the body, and these parts may undergo a coagulation process on the basis of platelet aggregation to form small thrombi.

[0003] The anticoagulant factors in the blood play an important role in the anticoagulant function. There are three main systems: thrombin III, protein C system and tissue factor pathway inhibitor (TFPI). What play an important role in ...

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