Method for producing tabling monoclonal antibody of anti CD25

Abstract

This invention provides a method for producing anti-CD25 chimeric monoclonal antibody, and corresponding expression vector and host cells. The method has such advantages as high expression level, and simple separation and purification. This invention also provides corresponding expression vector and engineering cells.

Description

technical field

[0001] The present invention relates to the field of genetic engineering, and more specifically relates to a method for producing an anti-CD25 chimeric monoclonal antibody, as well as an expression vector and a host cell used in the method. Background technique

[0002] Acute rejection after organ transplantation is common, which may cause necrosis of the transplanted kidney or decline in the function of the transplanted kidney. Therefore, drugs that reduce and attenuate these reactions are critical to the success of organ transplantation. Clinically, immunosuppressants are generally used to treat organ transplant patients to inhibit the occurrence of transplant rejection or reduce the degree of reaction. Therefore, kidney transplant recipients have to take immunosuppressive drugs for life to achieve: 1. Reasonable application to prevent and treat rejection (protect the alien kidney); 2. Maintain the patient's moderate immune resistance (protect the patient ...