36 results about "Transplanted kidney" patented technology

The invention discloses an intraoperative low temperature kidney protective bag and a using method thereof. The intraoperative low temperature kidney protective bag comprises a protective bag body, acool storage interlayer, inner layer folds, a cold storage infusion port, a kidney placing inlet and a kidney gate opening. The cool storage interlayer is arranged in the protective bag body; the inner wall of the protective bag body is provided with the inner layer folds, and the inner layer folds enclose to form an inner cavity; the inner cavity is filled with kidney protection liquid; the upperpart of the protective bag body is provided with the kidney placing inlet, a first elastic rubber ring is arranged at the kidney placing inlet, the lower part of the protective bag body is provided with the kidney gate opening, and a second elastic rubber ring is arranged at the kidney gate opening; the first elastic rubber ring and the second elastic rubber are both provided with elastic retractable ropes. The intraoperative low temperature kidney protective bag can ensure cryopreservation effect of a kidney during a kidney transplant operation process, and effectively improve preservation quality of the kidney. The intraoperative low temperature kidney protective bag is an easy-to-operate special refrigerated storage bag for transplanted kidney, and can be specially used in a kidney transplant operation.

The invention relates to an early diagnosis kit and an early warning kit, in particular to an early diagnosis and early warning kit for transplanted kidney rejection reaction and a use method of the kit, and further to some proteins applied in the early warning of transplanted kidney acute rejection reaction or transplanted kidney rejection reaction. By applying a liquid protein chip (luminex) detection technique, the expression level of 96 cytokines / chemokines and receptors thereof in 266 serum samples from 142 patients having acute transplanted kidney rejection reaction and transplant recipients with stable function of transplanted kidneys are monitored and comprehensively analyzed. An early warning system for the transplanted kidney rejection reaction comprising at least one combined marker and an early diagnosis system for the transplanted kidney rejection reaction comprising at least one factor combined marker are established. The prediction and accuracy rate of the acute rejection achieve 98.6%, and the internal cross validation rates are 97.1% and 97.2%, respectively.

The invention discloses a process storage cooling apparatus, particularly a process storage cooling apparatus for a robot kidney transplantation operation, belongs to the technical field of design and manufacture of medical surgical consumables, and provided is a kidney transplantation operation process storage cooling apparatus capable of maintaining a transplanted kidney always in a desired low temperature environment in a patient body during the whole organ implantation operation. The process storage cooling apparatus comprises a low temperature circulation cooling system and a kidney receiving cavity, wherein a through hole is arranged on the bottom portion of the kidney receiving cavity, the kidney receiving cavity is maintained in a low temperature environment having a temperature of not more than 5 DEG C by the low temperature circulation cooling system, and during kidney transplantation, a transplanted kidney is placed at the surgical position in a patient body through the kidney receiving cavity, and the to-be-connected kidney pedicle extends out of the kidney receiving cavity through the through hole.

The present invention provides a method for gene transfer through the vascular system or a ureter. In a preferable embodiment of this invention, an aqueous solution containing an expression vector comprising a desired gene is injected through the vascular system or a ureter of a desired organ to introduce the gene into the injected area. Specifically, the method of the present invention is effective for ex vivo gene therapy wherein the hepatocyte growth factor (HGF) gene is introduced by electroporation into a kidney to be transplanted. According to the present invention, the HGF functions continuously on the kidney to be transplanted, and the HGF within the treated area does not affect other organs of the body. Thus, the method of the present invention is a safe and effective treatment for maintaining the survival of transplanted kidneys.

The invention provides a non-invasive detection system for a transplanted kidney immune state, and belongs to the technical field of post-renal transplantation ultrasonic diagnosis automatics. The non-invasive detection system for the transplanted kidney immune state includes: a data management module for acquiring personal data of a patient and one or more pieces of ultrasonic detection data according to identity recognition information of the patient by means of networking; an ultrasonic connection module for accessing an ultrasonic detector and importing current ultrasonic detection data ofthe transplanted kidney; an analysis processing module for selecting a computing model according to the personal data of the patient, and acquiring an analysis result of the ultrasonic detection data; and a result output module for formatting the analysis result and outputting the result according to the transplanting time of the patient and the analysis processing result. Accordingly, the non-invasive detection system can collect and acquire patient clinical data and ultrasonic detection data, perform automatic / semi-automatic module combination, perform automatic classification operation, and output the image-text integrated result, can lower the working load of doctors, and can facilitate result understanding.

The invention discloses a photoacoustic ultrasonic bimodal imaging system and method for transplanted kidney. The system comprises a pulse laser which is used for the transplanted kidney to generate an photoacoustic signal; the ultrasonic transducer is used for receiving the photoacoustic signal and converting the photoacoustic signal into an electric signal; the ultrasonic transceiving module isused for transmitting and receiving electric signals; a photoacoustic signal processing unit; a data collection unit; and the computer is used for controlling the pulse laser and the ultrasonic transceiving module, performing image reconstruction on the digitized photoacoustic signal to obtain a photoacoustic image, and performing color coding on the photoacoustic image to realize development of functional information such as transplanted kidney microvascular distribution, intravascular blood oxygen content, oxygenated / deoxygenated hemoglobin content and the like. Structural imaging of the transplanted kidney can be achieved, functional information such as castruty distribution, intravascular blood oxygen content and oxygenated / deoxygenated hemoglobin content of the transplanted kidney canbe provided, and namely simultaneous real-time imaging of the structure and the function of the transplanted kidney is achieved.

The invention relates to a preparation method and an application of a regulatory T cell and belongs to the field of molecular biology and biological medicine. The preparation method of the regulatory T cell comprises the following step of preparing the regulatory T cell via TGF (transforming growth factor)-beta in-vitro induction. The invention further provides the application of the regulatory T cell generated by the TGF-beta in-vitro induction in preparing (a) a medicine of preventing and treating antibody mediated transplanted kidney rejection and injuries, or (b) a medicine of improving survival of the transplanted kidney. The use of the regulatory T cell generated by the TGF-beta in-vitro induction for a mouse antibody mediated acute transplanted kidney rejection model proves that the regulatory T cell can prevent and treat the antibody mediated transplanted kidney rejection and injuries, improve the survival of the transplanted kidney and reduce inflammatory cell infiltration.

The invention discloses a metabolic composition for pre-operative early warning of kidney delayed graft function of a donor donated after cardiac death. The metabolic composition is prepared from oneor more selected from seven metabolites including ascorbic acid, diaminopimelic acid, cinnamic acid, isobutyl-isobutyrate, androstenedione, 3-oxocaprylic acid and uroporphyrin. The invention further discloses a screening method of the metabolic composition. In the screened metabolic composition, the binding indication rate of ascorbic acid and diaminopimelic acid is 0.823, and the binding indication rate of cinnamic acid, isobutyl-isobutyrate, androstenedione, 3-oxocaprylic acid and uroporphyrin is 0.98.

The invention discloses a ureteral stent special for kidney transplantation. The ureteral stent comprises a stent body, a first curly tube, a tube opening, an intercepting tube, a second curly tube, aguide tube, a bent guide wire, a through hole, a protrusion, an intercepting tube wall, a limiting block, a rubber ring, a round hole, an intercepting cavity, a water blocking ball, a mounting cap and an elastic supporting body. The bent guide wire is made of a plastic material, so that the second coiled tube can be stably fixed in a renal pelvis of a transplanted kidney, the protrusions are allrubber protrusions and can block calculus particles, the stent body is made of a medical silica gel material, the water blocking ball is matched with the round hole, and the stent body is made of a medical silica gel material. The diameter of the water blocking ball is larger than the maximum diameter of the round hole, the phenomenon of urine backflow is prevented conveniently, the smooth layer is arranged in the stent body, a smooth inner surface is provided for the ureteral stent conveniently, smooth passing of fluid is facilitated, and the smoothness of an inner cavity of a tube body of the ureteral stent is improved.

The use of (R).-ibuprofen methanesulfonamide is described for the preparation of medicaments for the treatment and prevention of functional injury resulting from rejection reactions of transplanted organs. In particular, the use of non-toxic salts of (R)-ibuprofen methanesulfonamide, such as the (L)-lysine salt, is described for the prevention and the treatment of rejection reactions of transplanted kidneys.

The invention discloses an ice-temperature storage method of transplanted kidney and organs. The method specifically comprises the following steps of: carrying out ether anesthesia in trachea; taking the upper abdomen median incision, cutting left or right kidney as the donor kidney; intubating into the renal artery immediately after cutting the donor kidney; filling 0-1 DEG C of organ protective liquor by virtue of pushing force of an injection pump, wherein the filling flow rate is 2.7 ml / s-2.8ml / s, and the average cooling velocity is 6.00 DEG C / minute-6.33 DEG C / minute; and stopping the filling when the temperature of the filled protective liquid flowing out of the renal veins is 1.5 DEG C-2.5 DEG C; placing the kidney in a sterilized film bag with 300ml of organ protective liquid for sealing after filling, and storing the kidney in a constant-temperature box of 0 + / -0.15 DEG C. The ice-temperature technology is adopted, so that the organ tissue is lowered in phase-transition temperature and not frozen, so that the delay storage of the transplanted organ is realized; and the ice-point limit temperature is adopted for realizing the delay storage of the transplanted organ and to recover the good function of the transplanted organ.

The invention relates to the technical field of medical instruments, in particular to a continuous refrigeration system for kidney transplantation operation. The continuous refrigeration system comprises a kidney bag, a kidney bag opening, a kidney portal opening, an in-vitro ring, an in-vivo ring, a pull wire, an inner pull ring and an outer pull ring, the kidney bag opening is formed in the upper end of the kidney bag, a transplanted kidney and smoothie mixture enters the kidney bag through the kidney bag opening, the kidney portal opening is formed in the lower end of the kidney bag, the in-vitro ring is annularly arranged at the kidney bag opening and enables the kidney bag opening to be supported and expanded, the in-vivo ring is arranged on the kidney bag in an annular mode and arranged in the body of a patient of the transplanted kidney, the outer surface of the kidney bag is connected with the pull wire, the two ends of the pull wire protrude out of the kidney bag, the lower end of the pull wire is connected with the inner pull ring, and the upper end of the pull wire is connected with the outer pull ring. The kidney bag is pulled into the in-vivo kidney anastomosis position through the inner pull ring, the kidney bag is pulled out through the outer pull ring after an operation, and the problem that a kidney supply low-temperature protection bag is inconvenient to placein a human body and take out after the operation is solved.

The invention provides novel healthy kidney biomarkers useful in the monitoring of renal function and in the prognosis and diagnosis of renal dysfunctions, especially those related to graft rejection. The invention further relates to methods for aiding in the evaluation, and design of personalized therapies in transplantation nephrology.

The invention relates to the technical field of medical devices, in particular to a continuous refrigeration system for kidney transplantation operations, including: a kidney bag, a kidney bag opening, a kidney door opening, an extracorporeal ring, an inner ring, pull wires, an inner pull ring, and an outer pull ring; The upper end of the bag is opened with a kidney bag opening for the transplanted kidney and smoothie mixture to enter the interior of the kidney bag. The lower end of the kidney bag is opened with a kidney door opening. The opening of the kidney bag is supported and expanded, the upper ring of the kidney bag is provided with an internal ring, and the internal ring is placed in the body of the patient whose kidney has been transplanted. Pull ring, the outer pull ring is connected to the end of the pull wire. In the present invention, the kidney bag is pulled into the kidney anastomosis position in the body through the inner pull ring, and the kidney bag is pulled out through the outer pull ring after surgery, which solves the problem of the low temperature protection bag for the kidney being placed. It is inconvenient to enter the human body and take it out after surgery.

The invention relates to an early diagnosis kit and an early warning kit, in particular to an early diagnosis and early warning kit for transplanted kidney rejection reaction and a use method of the kit, and further to some proteins applied in the early warning of transplanted kidney acute rejection reaction or transplanted kidney rejection reaction. By applying a liquid protein chip (luminex) detection technique, the expression level of 96 cytokines / chemokines and receptors thereof in 266 serum samples from 142 patients having acute transplanted kidney rejection reaction and transplant recipients with stable function of transplanted kidneys are monitored and comprehensively analyzed. An early warning system for the transplanted kidney rejection reaction comprising at least one combined marker and an early diagnosis system for the transplanted kidney rejection reaction comprising at least one factor combined marker are established. The prediction and accuracy rate of the acute rejection achieve 98.6%, and the internal cross validation rates are 97.1% and 97.2%, respectively.

The present invention provides an organ preservation solution and its preparation method and application. The preservation solution contains soluble TNF receptor and soluble IL-1 receptor or a carrier expressing the receptor. The application of the preservation solution can effectively reduce the host-versus-graft and graft-versus-host reactions in the process of organ transplantation, prolong the survival time of the graft, and improve the survival rate of the graft in the host body, so it can be effectively applied to organ transplantation, including Transplantation of vital organs such as kidneys, livers, and hearts.

The invention discloses a marker for predicting the range and depth of kidney infection of polyoma virus and application thereof. Experiments verify that the combined detection of 58K Golgi protein and polyoma virus specific antigen has important application value in predicting the range and depth of transplanted kidney infection and prognosis of kidney transplantation patients, and is helpful forrisk stratification of infection and adoption of appropriate treatment strategies. The sample adopted by the detection strategy of the invention is urine sediment cells, which can be realized by non-invasive means, and the operation is simple, and most of the processes can be automated and almost can be carried out in each transplantation center.

The invention provides an application of a recombinant human myeloid-derived growth factor in preparation of a medicine for treating renal ischemia reperfusion injury. The recombinant human medullary-derived growth factor can reduce serum creatinine and urea nitrogen levels of mice suffering from renal ischemia reperfusion injury, improves renal functions after renal ischemia reperfusion injury, has a kidney protection effect and can relieve oxidative stress injury caused by renal ischemia reperfusion injury. The traditional Chinese medicine composition brings hopes for improving the life quality of kidney transplantation patients and prolonging the survival time of transplanted kidneys, and also has a certain far-reaching influence on prevention and treatment of ischemia reperfusion injuries of other tissues and organs.

The invention provides a transplanted renal function test nucleotide chip, consisting of a matrix nylon membrane (1), a spotting area (2) and an oligonucleotide probe (3), which is fixed to the surface of the nylon membrane (1) and is distributed in a matrix way after modification and hybridization. The oligonucleotide probe (3) is equipped with the sequence of SEQ ID NO 1. And a contact-type printing is adopted. Synthesized by BIO BASIC INC (Canda) company, a 70bp oligonucleotide fragment, the specific of which represents one gene, is spotted on the nylon membrane. With a reasonable design, the invention can test the transplanted renal function early, rapidly, cheaply and precisely. And the invention can be applied to the transplanted renal function test.

The invention discloses a postoperative monitoring device for a kidney transplantation recipient, and the device comprises: a data collection unit which is used for collecting peripheral blood transcriptome data of the kidney transplantation recipient; a database unit which is used for storing the peripheral blood transcriptome data; and a support vector machine unit which is used for generating a monitoring report based on the peripheral blood transcriptome data. Study data pre-stored in a database unit are called through the support vector machine unit, a classifier model is constructed and tested, and the classifier model predicts the probability of occurrence of postoperative acute rejection of a kidney transplantation recipient based on peripheral blood transcriptome data of the kidney transplantation recipient and generates a detection report. The kidney function condition of a kidney transplantation recipient is accurately monitored while non-invasive detection is achieved, and meanwhile a detection report can provide a theoretical necessary basis for further confirmation and diagnosis of transplantation kidney needle biopsy.

A system (100) for noninvasively detecting an immune state of a transplanted kidney is applicable to the technical field of ultrasonic diagnosis automation after a kidney transplantation operation. The system (100) for noninvasively detecting an immune state of a transplanted kidney comprises: a data management module (10), used for obtaining, by means of networking, personal data and ultrasonic detection data of a patient for one or more times of detection according to identity recognition information of the patient; an ultrasonic connection module (20), used for accessing an ultrasonic detector and importing current ultrasonic detection data of the transplanted kidney; an analysis and processing module (30), used for selecting a computing model according to the personal data of the patient, and obtaining an analysis result of the ultrasonic detection data; and a result output module (40), used for formatting the analysis result and outputting the result according to the transplanting time of the patient and the analysis processing result. Accordingly, the system (100) collects and obtains clinical data and ultrasonic detection data of a patient, performs automatic / semi-automatic module combination, performs automatic classification operation, and finally, outputs an image-text integrated result, reduces the working load of a doctor, and facilitates result understanding.

The present application describes and claims a novel method for identifying and stratifying the risk of developing a BK virus nephropathy (“Nx BK-v” below) in patients having undergone a kidney transplant. This method uses an index combining at least three bio-markers: i) the intensity of the anti-BK-v memory T lymphocyte response (memory T lymphocytes which are specific to v, hereinafter “LTm anti-BK-v”), ii) the number of occurrences of incompatibility in the HLA alleles of class I and class II between the donor and the recipient of the graft, taking into account iii) the viral charge of the BK-v virus in the whole blood of the patient. The present method allows a very precise evaluation of the risk of developing an Nx BK-v during the months following the test with the aim of optimising the immuno-suppressive treatment in order to better preserve the transplanted kidney.

The invention discloses a metabolic composition for pre-operative early warning of transplanted kidney delayed rework of a donation receptor after cardiac death. The metabolic composition is composedof one or more than one of four metabolites including glucuronic acid, ganglioside, physalis angulata sterol and thiophenol. The invention further discloses a screening method of the pre-operative early warning of transplanted kidney delayed rework of donation receptor after cardiac death. In the screened metabolic composition, the combination indication rate of glucuronic acid and ganglioside is0.885, and the combination indication rate of physalis angulata sterol and thiophenol is 0.953.

The invention provides a transplanted renal function test nucleotide chip, consisting of a matrix nylon membrane (1), a spotting area (2) and an oligonucleotide probe (3), which is fixed to the surface of the nylon membrane (1) and is distributed in a matrix way after modification and hybridization. The oligonucleotide probe (3) is equipped with the sequence of SEQ ID NO 1. And a contact-type printing is adopted. Synthesized by BIO BASIC INC (Canda) company, a 70bp oligonucleotide fragment, the specific of which represents one gene, is spotted on the nylon membrane. With a reasonable design, the invention can test the transplanted renal function early, rapidly, cheaply and precisely. And the invention can be applied to the transplanted renal function test.

The present invention provides a kidney production method including a step of tissue-specifically removing a metanephric mesenchyme of a metanephros of a non-human animal; a step of transplanting, into the metanephros, a kidney precursor cell derived from a non-human animal which is allogeneic or xenogeneic to the non-human animal; and a step of advancing development of the metanephros, which is a step in which the transplanted kidney precursor cell is differentiated and matured to form a part of the kidney.

The invention discloses a metabolic composition for early detection of transplanted kidney acute rejection. The metabolic composition is composed of one or more of 10 metabolite combinations of aminopterin, cane sugar, estrone, galactoinositol, cortisone, quinolone sugar, lactose, lyxose, palmitic acid and catechol. The invention further discloses a screening method of the metabolic composition. In the screened metabolite composition, the levels of aminopterin, cane sugar, estrone, galactoinositol, cortisone, quinolone sugar, lactose and lysose are increased, and the levels of palmitic acid and catechol are reduced, so that the metabolite composition has guiding significance for diagnosis of acute rejection; in the metabolite composition, the independent indication rate of the metabolite is 0.609-0.762, and the binding indication rate reaches 0.792; and in the metabolite composition, the core components are sucrose, galactoinositol, lactose, lyxose and quinolone sugar which jointly participate in a galactose metabolic pathway.

Provided is a method for evaluating the likelihood of the onset or progression of transplanted kidney chronic rejection or chronic kidney disease in a subject, wherein the amount of SYT17 protein in a urine-derived sample collected from the subject is used as an index. Furthermore, the present invention provides a pharmaceutical composition for preventing and / or treating chronic rejection of transplanted kidney or chronic kidney disease, comprising at least one substance selected from the group consisting of a specific antibody against SYT17, a derivative thereof, and an inhibitory nucleic acid against SYT17, and also comprising at least one substance selected from the group consisting of a specific antibody against SYT17, a derivative thereof, and an inhibitory nucleic acid against SYT17. Provided is a test kit for evaluating the likelihood of the onset or progression of transplanted kidney chronic rejection or chronic kidney disease, the test kit comprising an antibody specific to SYT17 or a derivative thereof.

The invention discloses a kidney transplantation device, which comprises a supporting platform, a lifting mechanism, a clamping mechanism and a cutting mechanism. The function of the supporting platform is to carry the whole device. The lifting mechanism moves the scalpel to the required Height, the clamping mechanism realizes the cutting movement of the scalpel through the gear and the lead screw, and then turns the scalpel upwards through the gear movement to clamp the kidney. The function of the cutting mechanism is to clamp the blood vessel and cut the blood vessel. The structure of the device Simple, safe, reliable, and highly automated.

The invention discloses a metabolic composition for preoperative early warning and delayed recovery of transplanted kidneys of donated donors after cardiac death, which consists of ascorbic acid, diaminopimelic acid, cinnamic acid, ureidoisobutyric acid, androstenedione, One or more of the seven metabolites of 3‑oxoctanoic acid and uroporphyrin. The invention also discloses a screening method for the metabolic composition used for early warning of the delayed recovery of the transplanted kidney of the donated donor after cardiac death. The binding indicator rate was 0.823, and the binding indicator rate of cinnamic acid, ureidoisobutyric acid, androstenedione, 3‑oxoctanoic acid and uroporphyrin was 0.98.