Marker for predicting range and depth of kidney infection of polyoma virus and application thereof

A polyoma virus, range technology, applied in the field of biomedicine, can solve the problems of lack of criteria for quantitative detection of urine PyV-Haufen, high risk of bleeding, and inability to predict the depth and scope of PyV infection

Inactive Publication Date: 2020-12-29
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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AI Technical Summary

Problems solved by technology

However, needle biopsy is an invasive test with limitations including a high risk of bleeding and cannot be repeated frequently
Quantitative Plasma PCR detection of PyV-DNA is an indicator commonly used in clinical detection of PyV infection. Its negative predictive value (NPV) for predicting PyVAN is >99%, but the positive predictive value (PPV) is only 40%, which is relatively low and unstable Nor can the depth and extent of PyV infection in transplanted kidneys be predicted
[0005] Although it has been reported that the quantitative detection of PyV-Haufen in urine can predict the degree of injury and pathological stage of PyVAN, the high cost and limited availability of electron microscopy limit its potential for rou

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  • Marker for predicting range and depth of kidney infection of polyoma virus and application thereof
  • Marker for predicting range and depth of kidney infection of polyoma virus and application thereof
  • Marker for predicting range and depth of kidney infection of polyoma virus and application thereof

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Embodiment Construction

[0051] In order to understand the technical content of the present invention more clearly, the following embodiments are given in detail in conjunction with the accompanying drawings. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0052] 1 Materials and methods

[0053] 1.1 Study population

[0054] This is a retrospective cross-sectional study conducted in a single center. A total of 76 kidney transplant recipients who were diagnosed with polyomavirus-associated nephropathy (PyVAN) in the First Affiliated Hospital of Sun Yat-sen University from August 19, 2015 to November 25, 2019 were enrolled in the study. By. All patients were without any treatment, "bait cells" (Decoycell, urothelial cells with PyV inclusion bodies in the nucleus) Decoy cells ≥ 1 / 10 high power fields (HPFs). Patients with retransplanted kidneys or recurrent PyVAN were not enrolled. This ...

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Abstract

The invention discloses a marker for predicting the range and depth of kidney infection of polyoma virus and application thereof. Experiments verify that the combined detection of 58K Golgi protein and polyoma virus specific antigen has important application value in predicting the range and depth of transplanted kidney infection and prognosis of kidney transplantation patients, and is helpful forrisk stratification of infection and adoption of appropriate treatment strategies. The sample adopted by the detection strategy of the invention is urine sediment cells, which can be realized by non-invasive means, and the operation is simple, and most of the processes can be automated and almost can be carried out in each transplantation center.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to markers for predicting polyomavirus intrarenal infection and judging the scope and depth of infection and applications thereof. Background technique [0002] Polyomavirus infection and polyomavirus-associated nephropathy (PyVAN) is one of the serious complications leading to allograft renal insufficiency and graft loss. Among kidney transplant recipients, the incidence of PyVAN fluctuates between 1% and 10%, resulting in up to 50% loss of allografted kidneys. According to the Banff classification standard, PyVAN is divided into A, B and C stages. The 2-year survival rates of stage A, B and C transplanted kidneys were 90%, 70% and 50% respectively. At present, there is no clear and effective antiviral drug, so early diagnosis of PyVAN is very important. [0003] In addition, according to previous studies, during the development of PyVAN, polyoma virus (PyV) usual...

Claims

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Application Information

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IPC IPC(8): G01N33/573G01N33/569G01N33/68
CPCG01N33/56966G01N33/56983G01N33/573G01N33/6854G01N2333/025G01N2333/90241G01N2800/245G01N2800/347G01N2800/52
Inventor 黄刚邱江陈徐涛黄炀
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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