58 results about "Small intestinal epithelium" patented technology

The invention relates to the technical field of microorganisms, and particularly provides Lactobacillus reuteri and application thereof. The Lactobacillus reuteri provided by the invention is a canine-derived Lactobacillus reuteri strain which is separated from hindgut content of a healthy adult blue fox for the first time by an inventor, and the preservation number of the Lactobacillus reuteri isCCTCC NO: M 2018943. The strain has good growth performance, is high in tolerance to gastric juice and intestinal juice and good in adhesion to small intestine epithelial cells, can promote proliferation of dog intestinal tract beneficial bacteria, can inhibit growth of harmful bacteria, can enhance the immune function of pet dog organisms, has better effect than that of similar products in the market, can serve as an alternative strain of probiotic preparations, and is quite wide in application prospect.

The invention provides an establishment of humanized three-dimensional intestinal mucosa model and an application thereof. The model improves a traditional Caco-2 monolayer cell model mainly based on normal physiological structure characteristics of small intestinal epithelium, wherein on basis of adding extracellular matrix, the Caco-2 cells are co-cultured with mucus-secreting cells, interstitial cells and immune cells for a period of time to form a three-dimensional intestinal mucosa model being similar with structure and function of the small intestinal epithelium in vivo. By using the model, a membrane permeating capability at small intestine epithelium as well as a metabolism degree of oral drug molecules can be predicted. The model is mainly used for researching transmembrane transportation and absorption, and metabolism process of medicament molecules at the small intestine in vitro.

The invention belongs to the technical field of cell isolation and cultivation. In order to solve the problems that the chicken small intestinal epithelial cells are difficult to isolate and culture in vitro at present, the existing isolation process is troublesome and time-consuming, the obtained cells are low in purity and the like, the invention provides isolation and primary culture methods of the chicken small intestinal epithelial cells. The methods comprise the steps of taking chick embryo small intestines, cleaning the small intestines to remove mesenteries, digesting small intestine tissues by protease to obtain the chicken small intestinal epithelial cells, performing adherent difference culture on the cells, removing adherent parenchyma cells, collecting intestinal epithelial cells, inoculating by taking the inoculum density being about 6.5X105/mL for culturing, the chicken small intestinal epithelial cells obtained by culturing is maximum in quantity, low in death rate, and best in growing status. The primary culture is performed by adopting a culture solution in which chicken serum is added, and the growing status of the obtained cells is better. The chicken small intestinal epithelial cells which are good in growing status, normal in shape, high in activity are successfully cultured, the primary and isolation culture methods of the chicken small intestinal epithelial cells are successfully established, and the technical support is provided for follow-up tests.

The invention discloses a piglet small intestine epithelial cell classification and separation method, which includes the following steps: (1) a small intestine section which is 60cm to 90cm long is adopted, 100mL to 150mL of 37 DEG C preheated and oxygenated phosphate buffer is injected into the enteric cavity, both ends are clipped by hemostatic forceps, the small intestine section is then incubated in a 37 DEG C water bath oscillator for 30 minutes, and the speed of oscillation is 70 rotations per minute; (2) the phosphate buffer is removed, 100mL to 150mL of cell separation solution is injected, the small intestine section continues to be incubated in the 37 DEG C water bath oscillator for 2.5 hours, the speed of oscillation is 70 rotations per minute, and the cell separation solution is collected respectively at different time points and new cell separation solution is injected; (3) the collected cell separation solution is centrifuged at 400g under 4 DEG C for ten minutes, the obtained precipitate is intestinal epithelial cells, and the cells which are collected according to the sequence of incubation times are cells of different parts from the villus tip to the crypt bottom in sequence along a recess-villus axis. The cells separated by the invention provide a novel technical method for an in-vivo study on the small intestine epithelial cell differentiation mechanism, the absorption and metabolism of nutrition and the development of drugs for related diseases.

The invention relates to a sulfide quinone oxidoreductase intestinal tract directed expression vector and a cell line thereof, belonging to the field of biotechnology. The SQR (sulfur quinine oxidoreductase) gene is inserted into a eukaryotic expression vector pcDNA (deoxyribonucleic acid) 3.1 (1), so that a slow virus recombinant vector Lanti4-blockit-IFABP (intestinal fatty acid binding protein)-SQR-GFP (green fluorescent protein) can be built, and the primarily cultured pig intestinal epithelial cell is infected by the slow virus recombinant vector, so that the eukaryotic cell line of specific expression SQR-GFP protein can be built. In order to further prepare the somatic cell nuclear transfer clone trans-SQR gene, necessary transgenic biological materials and key technical system support can be provided for cultivating new transgenic pig species reducing the pollutant discharge.

The invention discloses a porcine epithelial cell oxidative stress model, an establishment method therefor and application of the porcine epithelial cell oxidative stress model. The establishment method for the porcine epithelial cell oxidative stress model comprises the following steps: inducing porcine epithelial cells by AAPH to continuously generate stable endogenous free radicals, detecting the oxidative damage degree of the cells, and determining a stress model of oxidative damage. According to the porcine epithelial cell oxidative stress model, the optimal concentration for constructingthe porcine small intestinal epithelial cell oxidative stress model induced by the AAPH is determined by a series of tests, and the oxidative damage is evaluated by the established oxidative stress model, so that the convenient and stable intestinal epithelial cell model is provided for further research of the influence of the oxidative stress on animal intestinal health, and the model has important significance for maintaining human health and reducing direct losses and indirect losses caused by oxidative stress in animal husbandry production.

The invention belongs to the field of pharmaceutical preparations, and relates to a calcium carbonate nanoparticle composition for improving oral absorption of insoluble drugs, wherein the compositioncomprises an insoluble drug, an amphiphilic polymer, an amphiphilic polymer linked to a substrate of a transporter expressed by small intestinal epithelial cells, calcium carbonate nanoparticles andan optional pharmaceutic adjuvant. According to the invention, the experiment results prove that the composition can obviously improve the oral absorption of insoluble drugs, and provides a novel preparation strategy for oral administration of insoluble drugs.

The invention provides an establishment method of a heavy metal oral bioaccessibility detection system. The establishment method comprises the following steps: 1) respectively establishing a human body external gastrointestinal digestion model and a human small intestine epithelial cell 3D model; 2) respectively applying substances containing different concentrations of heavy metals to the human body external gastrointestinal digestion model to simulate the gastrointestinal digestion process; 3) respectively applying the digestive juice containing the heavy metals to the human small intestineepithelial cell 3D model, and performing culturing for a preset time to simulate a small intestine absorption process; and 4) tracking changes of heavy metal concentrations and valence states in the human body external gastrointestinal digestion process and the small intestine epithelial cell absorption process, calculating oral biological accessibility of heavy metals, and establishing a method for predicting exposure risks based on the biological accessibility. The invention belongs to the technical field of food safety detection and evaluation, is suitable for detecting oral biological accessibility of heavy metals, and is also suitable for potential toxicity effect detection and exposure risk evaluation.

The invention discloses a method for constructing a conditional inducible expression AsCpf1 lentiviral vector. The invention also discloses a Tet-on-3G-pLVX-AsCpf1 lentivirus obtained by packaging thelentiviral vector and an application of the lentivirus to pig small intestine epithelial cell line construction. Pig small intestine epithelial cells are a cell material that is widely used in the study of intestinal nutrients and immunity. The present invention utilizes an AsCpf1-expressed lentivirus system regulated by a Tet-on-3G system to screen out an inducible-expressed stable-transfectionpig small intestine epithelial cell line. When Dox is added for induction, cells start a large amount of AsCpf1 expression within 24 hours, and when no Dox is added for induction, AsCpf1 expression disappears; and AsCpf1 decreases significantly after the Dox adding is stopped for 12h, AsCpf1 expression level is extremely low after the Dox adding is stopped for 24h and 48h, and no expression can beobserved after 72h.

The invention relates to novel calcium chelating peptide as well as a preparation method and an application thereof. According to the invention, an amino acid sequence of the calcium chelating peptideis YQEPVIAPKL. The calcium chelating peptide provided by the invention is safe and non-toxic, and has better physicochemical activity compared with a traditional calcium supplement, and the calcium chelating capability of the calcium chelating peptide reaches 28.4 mg/g; the collagen peptide can be supplemented while absorption and bioavailability of calcium in a human body are improved, absorption and transport of calcium in Caco-2 small intestinal epithelial cells can be promoted, and the collagen peptide can be used as a raw material for drug development and biological calcium supplement. According to the preparation method provided by the invention, the calcium chelating peptide is successfully obtained, fish product resources can be fully utilized, the operation is simple, and a new way is provided for high-valued utilization of tilapia bones.

The invention discloses a liquid calcium soft capsule and a preparation method thereof. The liquid calcium soft capsule comprises a capsule shell and contents in a capsule body, the capsule skin comprises 30%-50% of gelatin, 10%-30% of glycerol, 30%-50% of purified water and 1%-4% of a coloring agent; the content in the capsule body comprises 30%-50% of calcium citrate, 1%-5% of milk mineral salt, 0.1%-3% of casein phosphopeptides, 0.01%-2% of vitamin D3 and 40%-60% of soybean oil. Calcium citrate is used as a main calcium source, and milk mineral salt is used as a secondary calcium source. Calcium citrate is organic acid calcium, the absorption rate of the calcium citrate is higher than that of inorganic calcium, the content of citric acid in urine can be increased, the urine is acidified, and formation of calculi is inhibited. Milk mineral salt is separated from milk, irritation to intestines and stomach is small, and the calcium supplement effect cannot be reversed. Casein phosphopeptides are added and combined with Ca < 2 + > to form a soluble complex, so that Ca < 2 + > is prevented from being precipitated in neutral or weakly alkaline small intestines, and absorption and utilization of small intestine epithelial cells to Ca < 2 + > are promoted. Vitamin D3 is a hormone precursor acting on calcium and phosphorus metabolism, and can improve the absorption of the body to calcium and phosphorus.

The invention discloses an additive for improving the jejunum anti-oxidation capacity of piglets with intrauterine growth retardation, and a preparation method and application of the additive, and belongs to the technical field of feeds. The additive is prepared from the raw materials in parts by weight: 10-15 parts of edible oil, 10-20 parts of an antioxidant, 5-10 parts of a bactericide, 11-25 parts of an energy substrate, and 40-50 parts of a carrier. According to the feed additive, fatty acid is provided through edible oil, the energy substrate provides energy for intestinal epithelial cells, combined with the anti-oxidation effect of the antioxidant and the bactericidal effect of the bactericide, the raw materials are together used for improving the anti-oxidation ability of small intestinal epithelial cells of piglets with intrauterine growth retardation, and then the intestinal immunity and barrier function of piglets with intrauterine growth retardation are further improved.

The invention discloses a method for preventing intestinal radiation damage, wherein the intestinal stem cell genes Dclk1, Msi1, Lgr5, Bmi1 and Notch1 are up-regulated through TPA, the key signal proteins PKC-betaII and Erk1/2 as well as the protein p90RSK are further up-regulated, and the intestinal epithelial cell proliferation is promoted to prevent intestinal radiation damage.