9 results about "Small intestinal epithelium" patented technology

This invention discloses a method for in-situ preparation of calcium chelate peptides that promote intestinal calcium absorption and osteoblast development from fish scales using high-pressure homogenization-assisted enzymatic hydrolysis. The method uses fish scales as raw material, which are pulverized and then pretreated with high-pressure homogenization to break down the dense cross-linked structure of the scales. Pepsin is used as the hydrolytic enzyme to achieve efficient enzymatic hydrolysis of collagen and in-situ chelation with natural calcium, resulting in a one-step preparation of collagen calcium chelate peptides. The product has a calcium retention rate of ≥84.16%, is rich in affinity amino acids, and shows a ≥62.97% increase in calcium transport compared to calcium chloride in a small intestinal epithelial cell absorption and transport model, while being safe and non-cytotoxic. This invention is a green and efficient process that eliminates the need for decalcification steps, achieving a collagen calcium chelate peptide yield of ≥83.4%. It realizes the high-value utilization of aquatic by-products and can be directly applied to various calcium supplements such as food and health products that enhance intestinal calcium absorption and promote bone development, solving problems such as low bioavailability of traditional calcium preparations and severe calcium loss in existing fish scale utilization processes.

The application discloses an albumin active polypeptide composition for promoting skin mucosa wound healing, which comprises the following components: albumin active polypeptide, yeast-beta-glucan, concentrated wolfberry juice, preservative, thickening agent, sweetening agent and water. The albumin active polypeptide is encapsulated by a carrier formed by cross-linking of phosphorylated microcrystalline cellulose and glycidyltrimethylammonium chloride modified chitosan, which can well protect the albumin active polypeptide, prevent the degradation of the active polypeptide by complex gastrointestinal digestive enzymes, effectively improve the retention rate of the active polypeptide in the gastrointestinal environment, significantly increase the enrichment amount of the active polypeptide in the small intestinal epithelial cells, promote the absorption of the active polypeptide by the small intestinal epithelial cells, and slowly release in the body, and then be transported to the target through blood circulation, so that the bioavailability of the active polypeptide is improved, and the ability of the composition for promoting skin mucosa wound healing is improved.

The application discloses a pig beta-defensin-3 gene core promoter and a construction method and application thereof, and belongs to the technical field of genetic engineering. The sequence of the core promoter is shown as SEQ ID No. 1, the core promoter has significant promoter activity in pig small intestinal epithelial cells, and can be regulated by nutrients such as sodium butyrate and glutamine. The application also discloses a construction method for specifically amplifying the core promoter, which comprises the following steps: performing PCR amplification by taking pig small intestinal epithelial cell DNA as a template, taking the upstream primer and the downstream primer shown as SEQ No. 2-SEQ No. 3 as the primer, constructing a pMD-18T-pBD-3-P recombinant plasmid, and taking the recombinant plasmid as a template to amplify a core promoter fragment. The core promoter provides an excellent experimental system for studying the transcriptional regulation mechanism of the pig beta-defensin-3 gene, the nutrient response characteristics provide a new platform for studying the interaction between nutrition and immunity, and the core promoter has a wide application prospect in the field of healthy breeding of livestock and poultry.

The invention belongs to the technical field of application of microorganisms, and particularly relates to application of bifidobacterium in relieving intestinal inflammation caused by ETEC and a preparation of the bifidobacterium, the bifidobacterium is animal bifidobacterium B4, and the preservation number of the bifidobacterium is CCTCC No: M20251926. The microecological preparation of the animal bifidobacterium B4 is used for relieving intestinal inflammation caused by ETEC, in a test, after an enteritis diarrhea model of a mouse is successfully established through intraperitoneal injection of ETEC, and the animal bifidobacterium and the preparation of the animal bifidobacterium are used for intervention, it is found that intestinal tissue damage caused by ETEC induction can be effectively relieved, weight loss can be relieved to a certain extent, and the intestinal inflammation caused by ETEC can be relieved to a certain extent. The bifidobacterium animalis and the bacillus microecological preparation thereof can promote the development of small intestine epithelial cells, inhibit the colonization of pathogenic bacteria, enhance the barrier function of intestinal mucosa, improve the damage of ETEC to the form of intestinal tissues, and improve the intestinal health. And after intervention of the bifidobacterium animalis and the preparation thereof, the content of short-chain fatty acids such as acetic acid and butyric acid in the intestinal tract of the intestinal model mouse can be increased, and the ETEC-induced intestinal inflammation reaction can be relieved.

According to the preservation method for the glycocalyx ultrastructures of podocytes, endothelial cells and epithelial cells, a high-pressure freezing instrument and a freezing replacement instrument are adopted, millisecond-level glassy state fixation of a sample is achieved through physical freezing, low-temperature dehydration is combined, and the dual problems of chemical damage and charge interference are effectively avoided; the shape preservation effect of glycocalyx of glomerular podocytes, glomerular endothelial cells and small intestine epithelial cells is remarkably improved, the thickness and spatial arrangement of the glycocalyx are close to the physiological state, and dangerous chemicals are prevented from being used.

This invention discloses a method for preparing calcium chelating peptides that promote intestinal calcium absorption and their application in promoting bone development and preventing osteoporosis, belonging to the field of food biotechnology. Using fish scales, a byproduct of aquatic product processing, as raw material, this invention prepares collagen calcium peptide hydrolysates through enzymatic hydrolysis, and further chelates and enriches them to prepare collagen calcium peptide chelates. Highly active calcium chelating peptides that promote intestinal calcium absorption are identified by HPLC-MS / MS technology. Experiments show that the peptides are safe and non-toxic, and in a Caco-2 small intestinal epithelial cell model, they can promote intestinal calcium transport and absorption, significantly increasing the amount compared to the calcium chloride control group. Molecular docking confirms that the peptides can stably bind to the EGFR active pocket, mediating osteogenic activity through hydrogen bonds, hydrophobic interactions, and electrostatic interactions. Within a concentration range of 1-100 μg / mL, they can significantly promote osteoblast proliferation and differentiation activity and enhance the formation of osteogenic mineralized nodules, exhibiting significant intestinal calcium absorption and osteogenic activity.

The invention provides a preparation method of a diglyceride cheese emulsified sausage, and is characterized in that the method comprises the following steps: preparing a modified soybean protein isolate nanoparticle suspension, a diglyceride emulsion, curd, diglyceride cheese, pickled minced pork, emulsified minced meat and the diglyceride cheese emulsified sausage. The prepared diglyceride cheese emulsified sausage is a low-fat healthy product, more diglyceride can be oxidized and decomposed into energy in small intestine epithelial cells, fat accumulation is reduced, the diglyceride partially replaces concentrated milk fat in cheese, the calorie density of the product can be reduced, and the low-fat diet trend is met.

The present application relates to a kind of small molecule peptides derived from lactadherin and its application.The amino acid sequence of the small molecule peptide is any one shown in SEQ ID NO:1~SEQ ID NO:4.The small molecule peptide of the present application is non-toxic, safe, can be combined with Ca 2+ Specific binding, strong calcium chelating ability, can significantly improve the calcium transport amount of small intestinal epithelial cells, thereby having good activity of improving intestinal calcium absorption, and can be used in food (such as, infant food) and the like.

This invention belongs to the field of bioactive peptide preparation, specifically relating to an iron chelating peptide, its preparation method, and its application in the preparation of iron supplements. This study used soybean protein as raw material and screened four iron chelating peptides—EDEPFNLRSR, EDEPFNLR, DDLELKKIR, and EEQPLEVR—through enzymatic hydrolysis, purification, identification, and molecular docking techniques. Using a Caco-2 small intestinal epithelial cell model, it was found that all of the above iron chelating peptides significantly promoted iron transport and absorption, and significantly promoted Fe2+ absorption. 2+ Retention in the Caco-2 cell monolayer. The novel iron chelating peptides screened from soybean protein hydrolysates in this invention hold promise as a safe and effective iron supplement.