18 results about "Small intestinal epithelium" patented technology

The invention relates to the technical field of medicine screening, in particular to an active substance screening platform and application thereof. The active substance screening platform comprises a cell combination; the cell combination comprises immunoregulation screening cells and antioxidant screening cells; the immunoregulation screening cell is a mouse mononuclear macrophage RAW 264.7 carrying an NF-kappa B element conserved sequence and a reporter gene; the antioxidant screening cell is a porcine small intestine epithelial cell IPEC-J2 cell carrying an ARE element conserved sequence and a reporter gene. The invention provides a screening platform based on a specific cell combination, which can quickly obtain an active substance with immune regulation, antioxidation or two functions from a large number of candidate active substances, has the advantages of low cost and high efficiency, and has important application value.

The present application relates to a milk-derived phosphorylated peptide with the activity of improving intestinal iron absorption and its application in the preparation of iron supplements. The sequence of the milk-derived phosphorylated peptide is EESITRINKKIE, and the glutamic acid at the amino terminal of the sequence is connected with 1-3 phosphoserines. The milk-derived phosphorylated peptide can be combined with Fe 2+ Specifically bound, strong iron chelating ability, and can significantly improve the iron transport amount in the Caco-2 small intestinal epithelial cell model, thereby having good activity of improving intestinal iron absorption, and can be widely applied in the fields of drugs, health care products or food.

This invention discloses a method for in-situ preparation of calcium chelate peptides that promote intestinal calcium absorption and osteoblast development from fish scales using high-pressure homogenization-assisted enzymatic hydrolysis. The method uses fish scales as raw material, which are pulverized and then pretreated with high-pressure homogenization to break down the dense cross-linked structure of the scales. Pepsin is used as the hydrolytic enzyme to achieve efficient enzymatic hydrolysis of collagen and in-situ chelation with natural calcium, resulting in a one-step preparation of collagen calcium chelate peptides. The product has a calcium retention rate of ≥84.16%, is rich in affinity amino acids, and shows a ≥62.97% increase in calcium transport compared to calcium chloride in a small intestinal epithelial cell absorption and transport model, while being safe and non-cytotoxic. This invention is a green and efficient process that eliminates the need for decalcification steps, achieving a collagen calcium chelate peptide yield of ≥83.4%. It realizes the high-value utilization of aquatic by-products and can be directly applied to various calcium supplements such as food and health products that enhance intestinal calcium absorption and promote bone development, solving problems such as low bioavailability of traditional calcium preparations and severe calcium loss in existing fish scale utilization processes.

The invention discloses an efficient mouse small intestine epithelial cell isolated culture method. The method comprises the steps of fetal mouse small intestine tissue obtaining, flushing digestion, sediment obtaining through centrifugal separation, density gradient centrifugal purification, differential adhesion method purification, inoculated culture and culture medium replacement for further culture. During culture, a first culture medium and a second culture medium based on William's E basic culture media are sequentially used, and the first culture medium comprises fetal calf serum, EGF, L-glutamine, mannitol, methylprednisone, bovine insulin and double antibodies; the second culture medium is prepared from fetal calf serum, vitamin C, L-glutamine, resveratrol, sodium pyruvate, bovine insulin and double antibodies. The rapid adherent proliferation and long-term stable culture of the mouse small intestine epithelial cells on the 2D surface are realized through mutual cooperation of the two culture media, so that the separation and in-vitro culture method of the mouse small intestine epithelial cells, which is simple, easy to operate, stable and efficient, is established, and convenience is provided for metabolic research of various in-vitro drugs and inflammatory factors.

The application discloses an albumin active polypeptide composition for promoting skin mucosa wound healing, which comprises the following components: albumin active polypeptide, yeast-beta-glucan, concentrated wolfberry juice, preservative, thickening agent, sweetening agent and water. The albumin active polypeptide is encapsulated by a carrier formed by cross-linking of phosphorylated microcrystalline cellulose and glycidyltrimethylammonium chloride modified chitosan, which can well protect the albumin active polypeptide, prevent the degradation of the active polypeptide by complex gastrointestinal digestive enzymes, effectively improve the retention rate of the active polypeptide in the gastrointestinal environment, significantly increase the enrichment amount of the active polypeptide in the small intestinal epithelial cells, promote the absorption of the active polypeptide by the small intestinal epithelial cells, and slowly release in the body, and then be transported to the target through blood circulation, so that the bioavailability of the active polypeptide is improved, and the ability of the composition for promoting skin mucosa wound healing is improved.

This invention discloses a lncRNA associated with sheep resistance to F17 Escherichia coli diarrhea, the nucleotide sequence of which is shown in SEQ ID NO.1. Through high-throughput sequencing and in vitro validation experiments, this invention discovered that DRB1-AS1 is induced to express during F17 Escherichia coli infection of the sheep small intestine, participating in the host immune protection mechanism during the occurrence and development of sheep diarrhea. It demonstrates that downregulation of DRB1-AS1 expression can reduce the adhesion resistance of sheep primary small intestinal epithelial cells to F17 Escherichia coli. The preparation and application methods of DRB1-AS1 in the prevention or treatment of sheep F17 Escherichia coli diarrhea, anti-inflammatory drug development, or sheep diarrhea resistance breeding have extremely high market implementation potential.

The application discloses a pig beta-defensin-3 gene core promoter and a construction method and application thereof, and belongs to the technical field of genetic engineering. The sequence of the core promoter is shown as SEQ ID No. 1, the core promoter has significant promoter activity in pig small intestinal epithelial cells, and can be regulated by nutrients such as sodium butyrate and glutamine. The application also discloses a construction method for specifically amplifying the core promoter, which comprises the following steps: performing PCR amplification by taking pig small intestinal epithelial cell DNA as a template, taking the upstream primer and the downstream primer shown as SEQ No. 2-SEQ No. 3 as the primer, constructing a pMD-18T-pBD-3-P recombinant plasmid, and taking the recombinant plasmid as a template to amplify a core promoter fragment. The core promoter provides an excellent experimental system for studying the transcriptional regulation mechanism of the pig beta-defensin-3 gene, the nutrient response characteristics provide a new platform for studying the interaction between nutrition and immunity, and the core promoter has a wide application prospect in the field of healthy breeding of livestock and poultry.

The invention discloses a polyclonal antibody of porcine CENP-A protein as well as a preparation method and application of the polyclonal antibody, and relates to the technical field of bioengineering. The preparation method comprises the following steps: immunizing an animal by using a pig CENP-A protein, and collecting and separating to obtain serum; performing extraction and purification treatment on the serum to obtain the polyclonal antibody; the amino acid sequence of the pig CENP-A protein is as shown in SEQ ID NO. 1. The polyclonal antibody of the CENP-A protein is relatively good in specificity and relatively high in titer, and the polyclonal antibody of the CENP-A protein, provided by the invention, can be used for specifically recognizing endogenous CENP-A protein in pig tissues or pig small intestine epithelial cell lines in immunoblotting analysis, and can be applied to a centromere region sequence combined with CENP-A captured by ChIP-seq.

The invention belongs to the technical field of application of microorganisms, and particularly relates to application of bifidobacterium in relieving intestinal inflammation caused by ETEC and a preparation of the bifidobacterium, the bifidobacterium is animal bifidobacterium B4, and the preservation number of the bifidobacterium is CCTCC No: M20251926. The microecological preparation of the animal bifidobacterium B4 is used for relieving intestinal inflammation caused by ETEC, in a test, after an enteritis diarrhea model of a mouse is successfully established through intraperitoneal injection of ETEC, and the animal bifidobacterium and the preparation of the animal bifidobacterium are used for intervention, it is found that intestinal tissue damage caused by ETEC induction can be effectively relieved, weight loss can be relieved to a certain extent, and the intestinal inflammation caused by ETEC can be relieved to a certain extent. The bifidobacterium animalis and the bacillus microecological preparation thereof can promote the development of small intestine epithelial cells, inhibit the colonization of pathogenic bacteria, enhance the barrier function of intestinal mucosa, improve the damage of ETEC to the form of intestinal tissues, and improve the intestinal health. And after intervention of the bifidobacterium animalis and the preparation thereof, the content of short-chain fatty acids such as acetic acid and butyric acid in the intestinal tract of the intestinal model mouse can be increased, and the ETEC-induced intestinal inflammation reaction can be relieved.

According to the preservation method for the glycocalyx ultrastructures of podocytes, endothelial cells and epithelial cells, a high-pressure freezing instrument and a freezing replacement instrument are adopted, millisecond-level glassy state fixation of a sample is achieved through physical freezing, low-temperature dehydration is combined, and the dual problems of chemical damage and charge interference are effectively avoided; the shape preservation effect of glycocalyx of glomerular podocytes, glomerular endothelial cells and small intestine epithelial cells is remarkably improved, the thickness and spatial arrangement of the glycocalyx are close to the physiological state, and dangerous chemicals are prevented from being used.

The invention belongs to the technical field of food processing, and particularly relates to a secoisolariciresinol diglucoside pyrolysis product for improving the stability and bioavailability of alpha-linolenic acid in an emulsion system, and a preparation method and application of the secoisolariciresinol diglucoside pyrolysis product. The FLM hot processing product with efficient antioxidant activity is obtained by carrying out heat treatment on natural-form flax lignan (FLM) at 150 DEG C, and the FLM hot processing product can be directly applied to an emulsion system so as to improve the stability and bioavailability of alpha-linolenic acid. When the FLM hot processing product and the ALA are subjected to co-nanocrystallization to form the emulsion, the FLM hot processing product spontaneously adsorbs on an oil drop interface to improve the density of the emulsion drop interface, and the storage physical and chemical stability of the ALA emulsion is further improved based on the interface anti-oxidation effect. The FLM hot processing product and the gastrointestinal tract digestion product of the FLM further participate in synthesis of ALA micelles, ALA absorption is promoted by improving expression of protein related to absorption of ALA in small intestine epithelial cells, so that the bioavailability of ALA is improved, and efficient ingestion of plant-derived ALA is realized based on'total ingestion chain type regulation '.

This invention discloses a method for preparing calcium chelating peptides that promote intestinal calcium absorption and their application in promoting bone development and preventing osteoporosis, belonging to the field of food biotechnology. Using fish scales, a byproduct of aquatic product processing, as raw material, this invention prepares collagen calcium peptide hydrolysates through enzymatic hydrolysis, and further chelates and enriches them to prepare collagen calcium peptide chelates. Highly active calcium chelating peptides that promote intestinal calcium absorption are identified by HPLC-MS / MS technology. Experiments show that the peptides are safe and non-toxic, and in a Caco-2 small intestinal epithelial cell model, they can promote intestinal calcium transport and absorption, significantly increasing the amount compared to the calcium chloride control group. Molecular docking confirms that the peptides can stably bind to the EGFR active pocket, mediating osteogenic activity through hydrogen bonds, hydrophobic interactions, and electrostatic interactions. Within a concentration range of 1-100 μg / mL, they can significantly promote osteoblast proliferation and differentiation activity and enhance the formation of osteogenic mineralized nodules, exhibiting significant intestinal calcium absorption and osteogenic activity.

The invention provides a preparation method of a diglyceride cheese emulsified sausage, and is characterized in that the method comprises the following steps: preparing a modified soybean protein isolate nanoparticle suspension, a diglyceride emulsion, curd, diglyceride cheese, pickled minced pork, emulsified minced meat and the diglyceride cheese emulsified sausage. The prepared diglyceride cheese emulsified sausage is a low-fat healthy product, more diglyceride can be oxidized and decomposed into energy in small intestine epithelial cells, fat accumulation is reduced, the diglyceride partially replaces concentrated milk fat in cheese, the calorie density of the product can be reduced, and the low-fat diet trend is met.

The invention provides an emotional regulation substance intestinal brain targeting delivery system based on nano-liposome and a preparation method of the emotional regulation substance intestinal brain targeting delivery system. According to the research, a nucleic acid aptamer capable of specifically recognizing the small intestine epithelial cells is obtained through screening by utilizing an SELEX technology, a delivery system with a nano-liposome as a core shell, an emotion regulating substance as a core and the nucleic acid aptamer as a target is constructed on the basis of the nucleic acid aptamer, and the delivery system recognizes the small intestine epithelial cells in a targeted mode by means of the intestinal brain axis; the directional enrichment of emotion regulating substances in the brain position is realized, and scientific significance and application value are provided for related emotion control and treatment.

The present application relates to a kind of bovine milk beta-casein source intestinal zinc absorption promoting peptide and its application in the preparation of zinc supplement.The sequence of the intestinal zinc absorption promoting peptide is YPVEPF, which is not only non-toxic, high safety, but also can be combined with Zn 2+ Specific binding, strong zinc chelating ability, can significantly improve the zinc transport amount in Caco-2 small intestinal epithelial cell model, thereby having good activity of improving intestinal zinc absorption.The intestinal zinc absorption promoting peptide can be obtained by solid phase synthesis method, so it can be produced on a large scale, and the synthesized intestinal zinc absorption promoting peptide not only has high purity but also stable peptide quality, which can meet the current market demand.

The present application relates to a kind of small molecule peptides derived from lactadherin and its application.The amino acid sequence of the small molecule peptide is any one shown in SEQ ID NO:1~SEQ ID NO:4.The small molecule peptide of the present application is non-toxic, safe, can be combined with Ca 2+ Specific binding, strong calcium chelating ability, can significantly improve the calcium transport amount of small intestinal epithelial cells, thereby having good activity of improving intestinal calcium absorption, and can be used in food (such as, infant food) and the like.

The invention relates to a pharmaceutical preparation for protecting the intestinal health of chickens, and belongs to the technical field of veterinary drugs. The core component is an oligopeptide composition (molecular weight lt: 1000 Da) prepared by enzymolysis of tilapia fish skin, solen meat and scapharca subcrenata meat according to a ratio of 4: 3: 3. The active oligopeptide with a synergistic effect is obtained through trypsin enzymolysis and an ultrafiltration purification process, so that proliferation of chicken small intestine epithelial cells can be remarkably promoted, and efficient protection on toxin damage can be realized. Animal experiments show that after 1 mg / kg of the oligopeptide composition is added into chicks fed with aflatoxin B1 polluted feed (100 [mu] g / kg), growth inhibition and diarrhea increase of the chicks caused by aflatoxin B1 can be remarkably reduced.

This invention belongs to the field of bioactive peptide preparation, specifically relating to an iron chelating peptide, its preparation method, and its application in the preparation of iron supplements. This study used soybean protein as raw material and screened four iron chelating peptides—EDEPFNLRSR, EDEPFNLR, DDLELKKIR, and EEQPLEVR—through enzymatic hydrolysis, purification, identification, and molecular docking techniques. Using a Caco-2 small intestinal epithelial cell model, it was found that all of the above iron chelating peptides significantly promoted iron transport and absorption, and significantly promoted Fe2+ absorption. 2+ Retention in the Caco-2 cell monolayer. The novel iron chelating peptides screened from soybean protein hydrolysates in this invention hold promise as a safe and effective iron supplement.