Method for simulating mechanical stimulation of abnormal blood flow to calcification of valvular cells

Abstract

The invention discloses a method for simulating mechanical stimulation of abnormal blood flow to calcification of valvular cells and relates to the technical field of biological model construction. The method comprises the following steps: S1) adopting a collagenase digestion method for extracting valvular interstitial cells in an aortic valve tissue and preparing into a cell supernatant culture medium; S2) mixing a magnetic bead with an RGD peptide stoste and preparing into a RGD peptide attached magnetic bead; S3) adding the RGD peptide attached magnetic bead into the cell supernatant culture medium acquired in the step S1), abandoning supernatant, cleaning with PBS and then adding into a first culture medium for culturing, putting the first culture medium into a magnetic distortion instrument, simulating the mechanical stimulation of the abnormal blood flow to the valvular interstitial cells by regulating the magnitude, frequency and acting time of the magnetic force of the magnetic distortion instrument and observing the calcification state of the valvular interstitial cells. According to the invention, the micro-simulation for the action of the mechanical stimulation of the abnormal blood flow to the calcification of valvular cells is realized, and meanwhile, the time for constructing a calcification model by a pure calcification culture medium can be shortened.

Description

technical field [0001] The invention relates to the technical field of biological model construction, in particular to a method for simulating the calcification of valve cells by abnormal hemodynamic stimulation. Background technique [0002] At present, in the study of mechanical stimulation of aortic valve calcification, bioreactors are mainly used, such as tensile force bioreactors, bending force bioreactors, shear force bioreactors, and composite bioreactors, all of which study macroscopic abnormalities The calcification of valve tissue or valve cells by mechanical stimulation can only simulate part of the macroscopic mechanics, such as pure laminar flow shear force and turbulent flow shear force, and there is no way to completely simulate external abnormal mechanical stimulation. [0003] Specifically, in the normal calcification process, the mechanical stimulus can only directly act on the valve endothelial cells, and the mechanical stimulation is transmitted to the va...

AI Technical Summary

Problems solved by technology

The macroscopic mechanical model cannot rule out the influence of cytokines secreted by the valve endothelial cells on the calcification of the valve interstitial cells, so as to simply study the effect of mechanical stimulation on the valve interstitial cells; at the same time, the macroscopic mechanical model cannot completely simulate the extracellular matrix or It is the process by which valve endothelial cells transmit mechanical stimuli to valve interstitial cells

[0004] At the same time, to study the influence of macroscopic mechanical stimulation receptors on calcification, the operation is complicated, and complex devices need to be placed in the incubator for continuous cultivation. The calcification cycle of macroscopic mechanical stimulation culture is longer.

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