Application of caffeic acid phenethyl ester in treatment of CAVD

A technology of phenethyl caffeate and calcification disease, applied in the field of prevention and/or treatment of aortic valve calcification and phenethyl caffeate, can solve the problem of poor long-term effect, high perioperative mortality, and aggravated patients Family financial burden and other issues

Active Publication Date: 2020-04-17
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, surgery will inevitably bring higher risks to patients, such as high perioperative mortality rate, poor long-term effect, and at the same time increase the financial burden on patients' families. There are still many clinical trials to verify some promising pharmacological interventions that stop or slow its progression

Method used

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  • Application of caffeic acid phenethyl ester in treatment of CAVD
  • Application of caffeic acid phenethyl ester in treatment of CAVD
  • Application of caffeic acid phenethyl ester in treatment of CAVD

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Construction of a human valve mesenchymal cell calcification model induced by a commercial osteogenic induction medium

[0023] Human valve mesenchymal cells with a culture density close to 80% were starved overnight with 2% FBS+DMEM high-sugar culture solution, and then starved with a newly configured osteogenic induction medium (Cyagen, product number: HUXMA-90021). The calcification of the valvular interstitial cells was induced, and alizarin red staining was performed after 21 days of induction to investigate the effects of different treatments on the calcification of the valvular interstitial cells.

Embodiment 2

[0024] Embodiment 2, caffeic acid phenethyl ester inhibits calcification of human valve interstitial cells

[0025] The human valve interstitial cell calcification model constructed as in Example 1 above was adopted, and the human valve interstitial cells were divided into 3 groups, respectively control group (untreated, normal medium), OM group (osteogenic medium induction) and OM+CAPE group (caffeic acid phenethyl ester was added after osteogenic medium induction). Different concentrations of phenethyl caffeate (phenethyl caffeate was purchased from Selleck, catalog number: S7414) were used to treat human valve interstitial cells, and IC50 test, CCK8 determination and Alizarin red staining analysis were performed.

[0026] IC50 results as figure 1 As shown in c, CAPE showed clear signs of toxicity when the concentration exceeded 10 μM, therefore, 10 μM CAPE was used for further experiments. cck8 cell viability detection, the concentration of 10 μM caffeic acid phenethyl es...

Embodiment 3

[0027] Example 3, the gene protein expression of phenethyl caffeate inhibiting the calcification of human valve interstitial cells

[0028] The human valve interstitial cell calcification model constructed as in Example 1 above was adopted, and the human valve interstitial cells were divided into 3 groups, respectively control group (untreated, normal medium), OM group (osteogenic medium induction) and OM+CAPE group (caffeic acid phenethyl ester was added after osteogenic medium induction). Human valve mesenchymal cells were treated with 10 μM caffeic acid phenethyl ester at a final concentration (final concentration in culture medium), and osteogenesis-specific genes RUNX2 and ALP were detected at 24h and 48h.

[0029] Quantitative qRT-PCR results showed that the expression of ALP and RUNX2 (osteogenic differentiation marker gene) was significantly upregulated by osteogenic medium compared with the control group (*p figure 2 a), showing a statistically significant difference ...

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Abstract

The invention relates to the field of prevention and/or treatment of CAVD. The inventor finds that caffeic acid phenethyl ester (CAPE) can effectively down-regulate the expression of osteogenic differentiation marker genes RUNX2 and ALP in valvular interstitial cells to inhibit the calcification of the valvular interstitial cells, thereby suppressing or delaying the valve calcification; and thus application of caffeic acid phenethyl ester in preparation of the medicine for preventing and/or treating the individual CAVD is provided. Therefore, novel selection is provided for treating the CAVD by using a non-surgical method.

Description

technical field [0001] The invention relates to the field of prevention and / or treatment of aortic valve calcification, in particular to the use of caffeic acid phenethyl ester in the prevention and / or treatment of aortic valve calcification. Background technique [0002] Caffeic acid phenylethyl ester (CAPE), a natural polyphenolic compound mainly found in the bark of coniferous trees as well as propolis in bee hives, has been described by existing studies as being resistant to various pathologies such as infection, oxidative stress , inflammation, cancer, diabetes, neurodegenerative diseases and anxiety. Furthermore, CAPE has been shown to inhibit NF-κB and contribute to anti-inflammatory activity. However, there is no report on the relationship between phenethyl caffeate and aortic valve calcification. [0003] Calcification refers to a process in which a certain tissue in the human body undergoes necrosis under the action of some factors, and then calcium salts in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61P9/00
CPCA61K31/216A61P9/00
Inventor 许康董念国黄玉明刘名周廷文
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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