Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for improving calcification of artificial biological valve by means of coating

A biological valve and artificial technology, applied in the direction of heart valves, can solve problems affecting the normal biological function and service life of valves, loss of soluble protein, calcification and hardening of valve leaflets, etc., to eliminate potential risks, enhance stability, and reduce damage Effect

Active Publication Date: 2020-09-15
NINGBO JENSCARE BIOTECHNOLOGY CO LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biovalve treated with glutaraldehyde cross-links the collagen to increase tissue stability, the carboxyl, hydroxyl and other ionic groups of the collagen itself are exposed due to the loss of soluble protein, which interacts with intracellular calcium ions (Ca 2+ ) combined, through a series of biochemical reactions to generate hydroxyapatite precipitation, leading to calcification, and the surface of the valve leaflets will also become rough
The calcification and hardening of the valve leaflet further lead to tearing and damage of the valve leaflet, which ultimately affects the normal biological function and service life of the valve

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for improving calcification of artificial biological valve by means of coating
  • A method for improving calcification of artificial biological valve by means of coating
  • A method for improving calcification of artificial biological valve by means of coating

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0048] The preparation of amino silicone oil emulsion can be carried out as follows:

[0049] Take a certain amount of deionized water and add it to a 250ml three-necked flask, add non-ionic emulsifier alkylphenol polyoxyethylene ether TX-10 and fatty alcohol polyoxyethylene ether AEO, according to the compounding ratio 1 / 2 to 2 / 3 drops Add, stir and mix evenly, then add an appropriate amount of co-emulsifier, continue to emulsify until clarification, slowly drop in the commercially available amino silicone oil raw material equivalent to 2 times the dosage of the compound emulsifier, and fully emulsify it under high-speed stirring After stirring for 2 hours, add a certain amount of acetic acid, slowly heat up to 30 degrees and mix evenly, slowly add a certain amount of water until the viscosity of the emulsion decreases, adjust the pH value to 6-7, and continue to stir for 60 minutes to obtain half Transparent microemulsion. The ammonia value range of the obtained amino silic...

Embodiment 1

[0056] The present embodiment provides the method for improving the calcification of prosthetic valve by means of coating, and this method comprises the following steps:

[0057] Step 1: Obtain fresh bovine pericardium material from the cattle farm;

[0058] Step 2: Pretreat the bovine pericardium material to remove the residual fat on the surface;

[0059] Step 3: Wash the bovine pericardium material in Hank's solution for 6 hours at a temperature of 8 degrees;

[0060] Step 4: Soak the cleaned pericardial material in 1% glutaraldehyde solution and react for 24 hours;

[0061] Step 5: washing the reacted pericardial material with saline;

[0062] Step 6: Soak the cleaned pericardial material in an amino silicone oil emulsion with an ammonia value of 0.3mmol / g and a viscosity of 1000mpa.s; soak for 3 hours at room temperature;

[0063] Step 7: Wash the pericardium after the above treatment with normal saline for 4 hours at a temperature of 5 degrees;

[0064] Step 8: use t...

Embodiment 2

[0074] A method for improving calcification of an artificial biological valve by means of a coating according to the present invention comprises the following steps:

[0075] Step 1: Obtain fresh pig heart bag;

[0076] Step 2: Pretreat the pig pericardium to remove excess fat and blood vessels on the surface;

[0077] Step 3: Wash the finished pig pericardium with physiological saline at a temperature of 4 degrees for 4 hours;

[0078] Step 4: Soak the pig pericardium in a 0.5% glyoxal solution for 48 hours and at a temperature of 8 degrees;

[0079] Step 5: Wash the fixed porcine pericardium with Hank's solution for 0.5 hour, at room temperature;

[0080] Step 6: Soak the pig pericardium in polysiloxane emulsion with an ammonia value of 0.2mmol / g, a viscosity of 2000mPa.s, soak for 3 days, and soak at a temperature of 37 degrees;

[0081] Step 7: The pig pericardium that has completed the above treatment is further washed with physiological saline for 1 hour and at a temp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed is a method for improving the calcification of an artificial biovalve by way of a coating. The method comprising first placing a pretreated biovalve material into an aldehyde-containing solution for a chemical cross-linking reaction, wherein the concentration of a solute having an aldehyde group in the aldehyde-containing solution is 0.1%-2% (w / w), and the reaction time is 0.5 hours to 2 weeks; then soaking the biovalve material in an amino silicone oil emulsion, wherein the amino silicone oil emulsion has an amino value range between 0.2-0.6 mmol / g and a viscosity between 500-5000 mPa.s, the soaking time is 0.2 hours to 1 week, and the temperature does not exceed 60ºC. In the method, by introducing a smooth and soft organic material coating into a biovalve leaflet material, the softness and surface smoothness of the valve leaflet are increased, friction is reduced and the risk of calcification is reduced, thereby extending the service life.

Description

technical field [0001] The invention belongs to the field of biomaterials, and in particular relates to a method for improving calcification of artificial biological valves by means of coating. Background technique [0002] Artificial biological valves are mainly divided into two categories, namely allogeneic biological tissue valves (including aortic valve, dura mater valve and fascia lata valve, etc.) and heterogeneous biological tissue valves (including porcine aortic valve and bovine pericardial valve, etc.). Compared with mechanical valves, biological valves have better hemodynamic function, do not require lifelong anticoagulation, have a lower incidence of thromboembolism, and can avoid fatal bleeding caused by anticoagulation. Most of the bioprostheses in clinical application are xenogeneic bioprostheses treated with glutaraldehyde. The principle is that the chemical cross-linking reaction occurs between the aldehyde functional groups in glutaraldehyde and the amino f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/24
CPCA61F2/2415
Inventor 曹鹏王娜郭改周意意李毅斌
Owner NINGBO JENSCARE BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com