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Separation, storage culture and amplification method of pouch fat interstitial cell

A preservation method and cell culture technology, which is applied in the field of separation and preservation of adipose interstitial cells in eye bags and their culture and expansion, can solve problems such as not seen, and achieve the effects of promoting repair, skin wound healing, and diabetic ulcer healing

Inactive Publication Date: 2012-07-18
EASTERN UNION STEM CELL & GENE ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The extraction and application of eye bag adipose stromal cells is a new topic, and no relevant reports have been seen so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] Embodiment: 1, the method for the separation and preservation of interstitial cell of eye bag fat, 1), under the purification workbench, the eye bag fat tissue is removed from the aseptic container, and put into a sterile vessel;

[0042] 2), adding a sterile phosphate buffer solution containing a bacteriostatic agent with an osmotic pressure of 270-330mOsm / kg, washing gently, and removing the phosphate buffer solution;

[0043] 3) Cut the tissue quickly with surgical scissors, and try to remove the yellow part until the length, width and height of the tissue block are all 1-2 mm;

[0044] 4) Add the tissue block and add phosphate buffer solution again, centrifuge at 200g-300g for 5 minutes, discard the upper layer of phosphate buffer solution, and select the lower layer of precipitated tissue block for later use;

[0045] 5) Take a 6-well to 24-well culture plate, implant one or more tissue pieces into each well with ophthalmic forceps, cover it with cover glass under ...

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PUM

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Abstract

The invention relates to a separation and storage method of a pouch fat interstitial cell, which comprises the following steps of: cutting to obtain a pouch fat tissue by adopting a surgical knife, extracting an interstitial tissue of the pouch fat tissue and separating the cell by using an adherent method. The culture and amplification method of the pouch fat interstitial cell comprises the following steps of: carrying out large-scale tissue culture by adopting a paper carrier technology, freezing and storing by using a definite and non-specific genetic component and preserving the cell for a long time. Through the separation and storage method, the pouch interstitial cell can be extracted, massively cultured and preserved for a long time; and the cultured interstitial cell can be used for promoting the healing of skin wound, the healing of diabetic ulcer, the repair of myocardial injury and the repair of hepatic injury.

Description

technical field [0001] The invention relates to a method for separating and preserving human eye bag adipose interstitial cells and a method for cultivating and expanding the eye bag adipose interstitial cells. Background technique [0002] The extraction and application of adipose stromal cells in eye bags is a new topic, and no relevant reports have been seen so far. Contents of the invention [0003] The purpose of the present invention is to address the deficiencies of the prior art, and to provide a method for the separation and preservation of interstitial cells of the eye bag and its culture and expansion method. The cells can be used to promote the healing of skin wounds, promote the healing of diabetic ulcers, promote the repair of myocardial damage, and promote the repair of liver function damage. [0004] Technical solutions of the present invention are as follows: [0005] 1. The method for separating and preserving the adipose interstitial cells of the eye b...

Claims

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Application Information

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IPC IPC(8): C12N5/077A01N1/02
Inventor 刘拥军吴明远黄家学刘洋施洁琦张丽丽
Owner EASTERN UNION STEM CELL & GENE ENG
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