Extraction analysis and application method of mair-cona active constituents
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A technology of active ingredients and analysis methods, applied in the field of analysis of active ingredients of Maca extract, can solve problems such as aging of the body and degeneration of brain physiological functions
Inactive Publication Date: 2016-06-22
CHANGCHUN UNIV OF CHINESE MEDICINE
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With the continuous increase of MAO activity, it will not only lead to the degenerati
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specific Embodiment approach 1
[0074] The preparation of maca extract comprises the following steps:
[0075] Step 1. Precisely weigh 500 g of dried and mature roots of Maca, and powder it into coarse powder (40 mesh);
[0076] Step 2, the obtained coarse powder was extracted three times with 10 times the amount of 70% ethanol under reflux, each time for 1 hour, filtered, and the extracts were combined;
[0077] Step 3: Recover the solvent with a rotary evaporator until there is no alcohol smell, and obtain a thick paste of the extract, evaporate the solvent to dryness, and use the maca extract as a dry paste for future use.
specific Embodiment approach 2
[0078] The preparation of maca extract comprises the following steps:
[0079] Step 1. Precisely weigh 500 g of dried and mature roots of Maca, and powder it into coarse powder (40 mesh);
[0080] Step 2, the obtained coarse powder was extracted three times with 10 times the amount of 85% ethanol under reflux, each time for 1 hour, filtered, and the extracts were combined;
[0081] Step 3: Recover the solvent with a rotary evaporator until there is no alcohol smell, and obtain a thick paste of the extract, evaporate the solvent to dryness, and use the maca total extract as a dry paste for later use.
specific Embodiment approach 3
[0082] The preparation of maca extract comprises the following steps:
[0083] Step 1. Precisely weigh 500 g of dried and mature roots of Maca, and powder it into coarse powder (40 mesh);
[0084] Step 2, the obtained coarse powder was extracted three times with 10 times the amount of 95% ethanol under reflux, each time for 1 hour, filtered, and the extracts were combined;
[0085] Step 3: Recover the solvent with a rotary evaporator until there is no alcohol smell, and obtain a thick paste of the extract, evaporate the solvent to dryness, and use the maca total extract as a dry paste for later use.
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Abstract
The invention relates to an extraction analysis and application method of mair-cona active constituents. The extraction analysis and application method of the mair-cona active constituents belongs to the technical field of bioengineering technology and comprises the following steps of (1) preparation of a mair-cona total extract; (2) preparation of five polarity extracts; (3) analysis of the mair-cona extract on DPPH free radical scavenging ability; (4) analysis of the mair-cona extract on testis interstitial cell proliferation and the function in promoting testis to prohibit cells from secreting testosterone; (5) analysis of monoamine oxidase immunocompetence in the mair-cona extract; (6) analysis of the acetylcholin esterase function in the mair-cona extract; (7) analysis of the anti-Glu-SY5Y damage function in the mair-cona extract. According to the extraction analysis and application method of the mair-cona active constituents provided by the invention, active parts of the mair-cona extract are analyzed, the best therapeutic effects of the active constituents on different diseases are obtained, the application of the mair-cona active constituents is promoted, and meanwhile, an effective reference is provided for orientating function development and chemical separation of mair-cona.
Description
technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an analysis method and application of active components of Maca extract. Background technique [0002] Maca has been eaten for thousands of years. In the continuous progress and development of human beings, Maca can not only provide nutrients for the human body as food, but also has the functions of strengthening the body, improving fertility, promoting sexual desire, improving sexual function, Anti-fatigue, anti-aging, anti-depression and other effects. [0003] Maca (Lepidium meyennii Walp.), also known as "Peruvian Ginseng", is an annual herb belonging to the genus Lepidium of the Brassicaceae family. Originally produced in the plateau mountains above 3,500 meters in Peru, maca needs a certain altitude to grow. At present, it is planted in Yunnan and Jilin in my country. Maca also has the effects of strengthening the body, improving fertility, promoting li...
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