47results about How to "Slow proliferation" patented technology

The invention relates to anti-proliferative aqueous extracts derived from plants, the extracts comprising compounds capable of inducing a plant organ into a state of dormancy or maintaining the organ in the state of dormancy. The invention further discloses cosmetic, pharmaceutical and agricultural compositions comprising the anti-proliferative extracts and use thereof.

This invention provides methods of regulating gene expression. An aptamer is positioned in a nucleic acid molecule along with a sequence encoding a transcriptional regulatory polypeptide. The aptamer disrupts translation of the transcriptional regulatory polypeptide when contacted with an aptamer-binding ligand. Gene expression levels can be either increased or decreased by the disclosed methods, depending on whether the transcriptional regulatory polypeptide is a repressor or activator, and the degree of the effect is dependent upon the dose of the ligand. Nucleic acid molecules, expression cassettes, expression vectors and cells useful in the gene regulation methods are also provided.

The invention relates to anti-proliferative agents derived from plants, wherein the agents are capable of inducing a plant organ into a state of dormancy or maintaining the organ in the state of dormancy. The invention further discloses compositions comprising the anti-proliferative agents and the use of said compositions to inhibit undesired or deleterious cell proliferation in plant or mammal tissue.

Compositions and methods that employ various combinations of such factors as retinoic acid signaling inhibitors, antioxidants, BMP4, VEGF, prostaglandin E₂ pathway stimulants, TPO, SCF, FLT-3, EPO, TGFβ1, p38 MAPK inhibitors, beta adrenergic receptor agonists, cell cycle inhibitors, RXR agonists, Cripto, and chromatin remodelers to drive differentiation of pluripotent stem cells towards primitive blood cells. Uses of such primitive blood cells are provided.

The present invention provides methods for stabilizing, reducing or eliminating cancer cells. In particular, the present invention provides prophylactically and/or therapeutically effective regimens for the prevention, treatment and/or management of cancer, the regimens comprising administering one or more cancer therapies to a subject to reduce a cancer cell population. The therapy(ies) in the prophylactically and/or therapeutically effective regimen can be administered at a lower dose than currently used or known to one of skill in the art and/or for a longer period of time and/or more frequently than currently administered or known to one of skill in the art.

The present invention is directed to compounds useful as cancer cell inhibitors, compositions containing such compounds and methods for inhibiting proliferation of electrically non-excitable cells.

The invention discloses a starting method for rapid and efficient short-cut nitrification. The method comprises the following steps: guiding inoculated sludge which is rich in nitrobacteria into an SBR (Sequencing Batch Reactor) reactor; guiding sewage into the SBR reactor in order that the concentration of the sludge in the reactor becomes 2,500-3,500mg/L, and controlling the temperature of the sewage at 30+/-1 DEG C and the pH of the sewage at 7.9-8.2; starting an aeration facility in the reactor in order that the concentration of dissolved oxygen becomes 0.8-1.2mg/L; performing aeration for 1.5-2.5 hours; stopping aeration, and starting to stir for 0.5-1.5 hours to uniformly mix the sludge and the sewage; repeatedly aerating and stirring operation for 1-5 times; standing the mixture for separating a solid from a liquid; and draining supernatant. By performing the short-cut nitrification in an intermittent aeration way, the growth of AOB (Ammonia Oxidizing Bacteria) can be better facilitated, a nitrification reaction is restrained, the energy consumption of a reaction device can be lowered effectively, and the starting of the short-cut nitrification of domestic sewage with low ammonia nitrogen content can be realized in comparison to the conventional continuous aeration.

Problems to be Solved Methods for enhancing survival and/or proliferation of neural stem cells and pharmaceutical compositions containing neural stem cells prepared by such methods, together with methods for assaying factors enhancing survival and/or proliferation of neural stem cells and methods for screening for such factors. Means for Solving the Problem Either Galectin-1 is overexpressed in neural stem cells or neural stem cells are cultured in a liquid medium containing Galectin-1. Pharmaceutical compositions containing Galectin-1-overexpressing neural stem cells and pharmaceutical composition containing Galectin-1, prepared by the aforementioned methods, improve higher cerebral functions damaged by cerebral ischemia. Further, by seeding neural stem cells at clonal concentrations and determining whether the seeded neural stem cells are capable of proliferating in an assay medium to be assayed, whether the factor enhances survival and/or proliferation of neural stem cells is assayed and a factor enhancing survival and/or proliferation of neural stem cells are identified using this assay method.

The invention discloses a rapid start method for an anaerobic ammonia oxidation reactor. The rapid start method comprises the following steps: by using a dynamic membrane bioreactor and by taking aerobic sludge and/or anaerobic sludge as inoculums, controlling the concentration of ammonia nitrogen and nitrite nitrogen of fed water, feeding a nutrient solution, filtering off the water by using a dynamic membrane, and enabling the reactor to continuously operate for at least 30 days, thereby achieving rapid domestication and enrichment of anaerobic ammonium oxidation bacteria inside the bioreactor. The rapid start method is applicable to anaerobic ammonia oxidation denitrification treatment on municipal sewage and industrial wastewater, and has the advantages of rapid start, high treatment efficiency, stable system operation, good effluent effect, low cost and the like.

The invention belongs to the technical field of seedling tissue culture, and relates to an oil palm tissue culture breeding method. The method comprises the steps of: taking young oil palm embryo as explant, disinfecting and inducing callus; conducting adventitious bud culture to the callus, and later conducting multiplication; conducting rooting culture to the multiplicated adventitious buds; and exercising rooted seedling, and transplanting into disinfected culture medium for culturing, thus obtaining oil palm regeneration plant. By taking young oil palm embryo as explant and obtaining regenerated plant through induced differentiation, the method solves the problems that the current oil palm has low multiplication differentiation rate, slow multiplication and incapability of meeting large-scale planting, effectively shortens the seedling tissue culture time, and has the characteristics of being simple in process, short in culture period, high in rooting rate, high in transplantation survival rate, and the like, thus establishing a good basis for industrialized and large-scale oil palm seedling, and providing precedent conditions for the oil palm industrialization development.

The present disclosure describes compositions for intratumoral administration which include a tissue-stabilizing agent and a therapeutic agent.

A human fibroblast modified foreign cell-removed epidermis substitute with high bioactivity and affinity is prepared through treating the human fibroblasts by mitomycin C, inoculating them on the surface of foreign cell-removed epidermis substitute, and external culture to become the epidermis substitute containing single or double layers of human fibroblasts.

The invention discloses a rapid startup device and method for normal-temperature shortcut nitrification of urban sewage with low C/N ratio. The device comprises an SBR (sequencing batch reactor), wherein an aeration device, a DO sensor, a stirring device and a pH sensor are arranged in the SBR; the SBR is connected with a water inlet pump and a water outlet pump through pipes; a sludge discharge pipe is arranged at the bottom of the SBR. Meanwhile, the rapid startup method for normal-temperature shortcut nitrification of the urban sewage with the low C/N ratio is disclosed and comprises the following steps: (1) inoculation; (2) sewage feeding; (3) aeration; (4) standing for discharging; (5) repeated treatment; (6) pH shock. Growth of AOB can be promoted better, nitrification is inhibited,energy consumption of a reaction device can be reduced effectively, startup period for shortcut nitrification is shortened, and rapid startup for shortcut nitrification of domestic sewage with low ammonia nitrogen content can be realized.

The invention discloses a method for separating and culturing vaginal epithelial cells. The method for separating and culturing vaginal epithelial cells comprises the following steps: shredding a tissue, first digesting the tissue with a digestive fluid A, then performing sieving with a 70-micron cell sieve, and collecting cells; adding a mixed digestive fluid into the remaining tissue for furtherdigestion, then performing sieving with a 70-micron cell sieve, and collecting cells; and centrifuging the two collected cells, washing the cells with a culture medium for a plurality of times, and spreading the cells in a Matrigel pre-coated cell culture plate to obtain the vaginal epithelial cells. According to the method, the vaginal epithelial cells are obtained by performing digestion and separation twice on the vaginal tissue by pancreatin containing EDTA and the mixed digestive fluid for the first time. The growth and amplification of the vaginal epithelial cells can be maintained by culturing the vaginal epithelial cells through an optimized KSFM culture medium.